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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (9): 1456-1463.doi: 10.3724/SP.J.1006.2020.94191

• RESEARCH NOTES • Previous Articles    

Control effects of different potato varieties (lines) and rice-potato rotation system on root-knot nematode

WU Chao(), LIU Xian-Wen, ZHANG Wei, WANG Qiong*(), GUO Hua-Chun*()   

  1. Tuber and Root Crops Research Institute, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China
  • Received:2019-12-09 Accepted:2020-04-15 Online:2020-09-12 Published:2020-05-08
  • Contact: Qiong WANG,Hua-Chun GUO E-mail:1371757245@qq.com;468050753@qq.com;ynghc@126.com
  • Supported by:
    China Agriculture Research System (Potato, CARS-09-15P).

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Abstract:

Root-knot nematode disease, induced by Meloidogyne incognita and Meloidogyne javanica, is one of the main reasons of crop failure in potato continuous cropping in Yunnan province. Variety resistance evaluation, rice-potato rotation model and dynamic changes were studied in order to assess the protect-and-control treatment of potato root-knot nematode disease. Variety resistance evaluation, rice-potato rotation model and dynamic changes were all studied in order to evaluate the protect-and-control treatment of potato root-knot nematode disease. The results from the variety resistance evaluation indicated that among the 18 potato cultivars (lines) no resistant materials were screened resistant to both Meloidogyne incognita and Meloidogyne javanica, whereas the 1002-1 line and Lishu 6 displayed better disease resistance, Qingshu 9 and Cooperation 88 were more sensitive to potato root-knot nematode disease. All kinds of the egg, larva and imago of root-knot nematodes were simultaneously detected in soil with the root-knot nematodes disease. However, after rice-potato rotation, the egg, larva and imago of root-knot nematodes were to disappear entirely in soil, and the root knot nematode disease was no longer occurred in the following potato planting process. Furthermore, the tuber yield per plant of Qingshu 9, Cooperation 88 and Lishu 6 compared with control were significantly different increased by 50.5%, 43.7%, and 26.0%, respectively. These results suggested rice-potato rotation could be an effective way to control potato root knot nematode disease. In addition, to further analyzed the mechanism of rice-potato rotation inhibiting root-knot nematode survival, we observed the dynamic changes of the amount on root knot nematodes in the case of soil flooding. The results showed that no different states of the insect state were detected until submerged more than 60 days. Therefore, we speculated prolonged soil flooding may be the main reason for inhibiting the growth of root-knot nematodes. It is a sustainable and effective method to control potato root-knot nematode by selecting rice-potato rotation with disease-resistant varieties.

Key words: potatoes, varieties (lines), rice-potato rotation cropping, root-knot nematode, control effect

Table 1

Root-knot nematode base per gram of soil (strip g-1)"

样品编号
Number of samples		 雌虫
Female worms		 幼虫和雄虫
Larvae and male worms		 卵
Eggs		 总数
Total
土样1 Soil samples 1 0.5±0.1 2.2±0.9 3.0±0.4 5.7±0.6
土样2 Soil samples 2 0.8±0.3 1.8±0.4 2.7±0.4 5.3±0.4
土样3 Soil samples 3 0.5±0 1.3±0.2 2.2±0.2 4.0±0.2

Table 2

Identification results of resistance to root-knot nematodes from two different potato varieties (lines)"

品种(系)
Variety
(lines)		 线虫类型
Type of
Meloidogyne
spp.		 根系Root 块茎Tuber
感病率
Susceptibility
rate (%)		 病情指数
Susceptible
index		 抗性
Resistance
level		 感病率
Susceptibility
rate (%)		 病情指数
Susceptible
index		 抗性
Resistance
level
1002-1 M.i 0 0 IM 0 0 IM
M.j 11.11 16.67 HR 2.40 1.60 HR
CIP 81 M.i 44.44 37.04 MR 3.40 2.49 HR
M.j 0 0 IM 0 0 IM
CIP 88 M.i 40 27.50 MR 3.68 3.07 HR
M.j 0 0 IM 0 0 IM
CIP 164 M.i 30 26.67 MR 3.42 3.08 HR
M.j 0 0 IM 0 0 IM
丽薯6号 M.i 20 20 HR 4.35 4.35 HR
Lishu 6 M.j 0 0 IM 0 0 IM
CIP 50 M.i 40 33.33 MR 10.19 5.09 HR
M.j 0 0 IM 0 0 IM
CIP 58 M.i 50 43.33 MR 15.22 9.06 HR
M.j 0 0 IM 0 0 IM
CIP 57 M.i 50 36.67 MR 10.10 9.60 HR
M.j 0 0 IM 0 0 IM
合作88 M.i 71.43 61.90 MS 22.09 12.79 HR
Cooperation 88 M.j 0 0 IM 0 0 IM
CIP 64 M.i 90 67.50 MS 33.88 21.21 HR
M.j 0 0 IM 0 0 IM
青薯9号 M.i 90 66.67 MS 36.54 22.60 HR
Qingshu 9 M.j 30 26.67 MR 8.91 7.92 HR
西海 35
Xihai 35		 M.i 80 62.50 MS 27.55 25.00 HR
M.j 0 0 IM 0 0 IM
2013-16D1 M.i 60 60 MS 29.63 27.78 MR
M.j 0 0 IM 0 0 IM
中薯20号 M.i 100 73.33 MS 42.31 28.85 MR
Zhongshu 20 M.j 40 30 MR 7.69 7.69 HR
3S-24 M.i 100 85.00 HS 41.77 29.11 MR
M.j 0 0 IM 0 0 IM
中薯18号 M.i 100 88.89 HS 51.39 33.10 MR
Zhongshu 18 M.j 80 70 HS 6.56 8.20 HR
滇薯701 M.i 70 55.00 MS 39.53 38.37 MR
Dianshu 701 M.j 0 0 IM 0 0 IM
滇同1号 M.i 100 83.33 HS 42.86 39.29 MR
Diantong 1 M.j 0 0 IM 0 0 IM

Table 3

Statistics of root-knot nematodes in tubers, roots, and soil after crop rotation"

作物
Crops		 块茎线虫
Nematodes
of tuber		 根系线虫
Nematodes
of root		 土壤线虫
Nematodes of soil		 相对防治效果
Relative control
effect (%)
一茬 青薯9号-青薯9号 Qingshu 9-Qingshu 9 + + +
First 滇杂36-青薯9号 Yunnan miscellaneous 36-Qingshu 9 - - 100
crop 合作88-合作88 Cooperation 88-Cooperation 88 + + +
Apr., 2017 滇杂36-合作88 Yunnan miscellaneous 36-Cooperation 88 - - 100
丽薯6号-丽薯6号 Lishu 6-Lishu 6 + + +
滇杂36-丽薯6号 Yunnan miscellaneous 36-Lishu 6 - - 100
1002-1-1002-1 + + +
滇杂36-1002-1 Yunnan miscellaneous 36-1002-1 - - 100
二茬 青薯9号-青薯9号 Qingshu 9-Qingshu 9 + + +
Second 滇杂36-青薯9号 Yunnan miscellaneous 36 -Qingshu 9 - - - 100
crop 合作88-合作88 Cooperation 88-Cooperation 88 + + +
Nov., 2011 滇杂36-作88 Yunnan miscellaneous 36-Cooperation 88 - - - 100
丽薯6号-丽薯6号 Lishu 6-Lishu 6 + + +
滇杂36-丽薯6号 Yunnan miscellaneous 36-Lishu 6 - - - 100
1002-1-1002-1 + + +
滇杂36-1002-1 Yunnan miscellaneous 36-1002-1 - - - 100
三茬 青薯9号-青薯9号 Qingshu 9-Qingshu 9 + + +
Third 滇杂36-青薯9号 Yunnan miscellaneous 36-Qingshu 9 - - 100
crop 合作88-合作88 Cooperation 88-Cooperation 88 + + +
Apr., 2018 滇杂36-合作88 Yunnan miscellaneous 36-Cooperation 88 - - 100
丽薯6号-丽薯6号 Lishu 6-Lishu 6 + + +
滇杂36-丽薯6号 Yunnan miscellaneous 36-Lishu 6 - - 100
1002-1-1002-1 + + +
滇杂36-1002-1 Yunnan miscellaneous 36-1002-1 - - 100
四茬 青薯9号-青薯9号 Qingshu 9-Qingshu 9 + + +
The 滇杂36-青薯9号 Yunnan miscellaneous 36-Qingshu 9 - - - 100
fourth 合作88-合作88 Cooperation 88-Cooperation 88 + + +
crop 滇杂36-合作88 Yunnan miscellaneous 36-Cooperation 88 - - - 100
Nov., 2018 丽薯6号-丽薯6号 Lishu 6-Lishu 6 + + +
滇杂36-丽薯6号 Yunnan miscellaneous 36-Lishu 6 - - - 100
1002-1-1002-1 + + +
滇杂36-1002-1 Yunnan miscellaneous 36-1002-1 - - - 100

Fig. 1

Effects on yield per plant, effective yield per plant, number of tubers per plant and infection rate of tuber in irrigation and drought rotation A: effects of yield level per plant from irrigation and drought rotation; B: effects of effective yield level per plant from irrigation and drought rotation; C: effects of number of tubers per plant from irrigation and drought rotation; D: effects of infection rate of tuber from irrigation and drought rotation. Q9: Qingshu 9; H88: Cooperation 88; L6: Lishu 6. Different lowercase letters indicate significantly different among treatments at P < 0.05. The results are the averages of the second and fourth crops."

Fig. 2

Dynamic variation of density of root-knot nematodes with immersion time in soil Density number ± number: the base density of root knot nematod."

[1] 潘柱, 梁石林, 梁冰. 信宜冬种马铃薯的气候条件分析. 气象研究与应用, 2012,33(S1):188-189.
Pan Z, Liang S L, Liang B. Analysis of climate conditions of winter potato in Xinyi. J Meteorol Res Appl, 2012,33(Sl):188-189 (in Chinese with English abstract).
[2] 蒙美莲. 中国马铃薯栽培生理研究进展. 见: 中国作物学会马铃薯专业委员会主编.中国马铃薯学术研讨会与第五届世界马铃薯大会论文集. 中国作物学会马铃薯专业委员会: 中国作物学会, 2004. p 1.
Meng M L. Research progress of potato cultivation physiology in China. In: Potato Professional Committee of Chinese Crop Society, eds. Symposium on Chinese Potato and Proceedings of the Fifth World Potato Congress. Potato Professional Committee of Chinese Crop Society: The Crop Science Society of China, 2004. p 1 (in Chinese).
[3] 张绍升. 植物线虫病害诊断与防治. 福州: 福建科学技术出版社, 1999. p 102.
Zhang S S. Diagnosis and Control of Plant Nematodes. Fuzhou: Fujian Science and Technology Press, 1999. p 102 (in Chinese).
[4] 陈品三, 彭德良, 郑经武. 马铃薯繁峙根结线虫新种(Meloidogyne fanzhiensisn sp.)之发现描述. 山西农业大学学报(自然科学版), 1990,10(1):55-60.
Chen P S, Peng D L, Zheng J W. Description of the Discovery of a new species of potato nematodes (Meloidogyne fanzhiensisn sp.). J Shanxi Agric Univ(Sci Edn), 1990,10(1):55-60 (in Chinese with English abstract).
[5] Garcia L E, Segura D M, Masuelli R W, Sanchezpuerta M V. Evaluating root-knot nematode infection in wild potatoes. Nematropica, 2014,44:31-36.
[6] 张云美. 我国根结线虫属(Meloidogyne Goeld, 1887)一新种. 山东大学学报(理学版), 1983, (2):88-95.
Zhang M Y. A new species of root knot nematodes (Meloidogyne Goeld, 1887) from China. J Shandong Univ(Nat Sci), 1983, (2):88-95 (in Chinese with English abstract).
[7] 杜宇, 丁元明, 寸东义, 曹云华, 周力兵, 刘忠善, 和捷. 输华马铃薯上哥伦比亚根结线虫风险分析. 植物保护, 2007,33(4):45-49.
Du Y, Ding Y M, Cun D Y, Cao Y H, Zhou L B, Liu Z S, He J. Risk analysis of Columbia root-knot nematode Meloidogyne chitwoodion potatoes in China. Plant Prot, 2007,33(4):45-49 (in Chinese with English abstract).
[8] 毛彦芝, 李春杰, 胡岩峰, 华萃, 尤佳, 王鑫鹏, 刘喜才, 杨耿斌, 王从丽. 评价黑龙江省主栽马铃薯品种及重要育种材料对北方根结线虫的抗性. 作物学报, 2017,43:1864-1869.
doi: 10.3724/SP.J.1006.2017.01864
Mao Y Z, Li C J, Hu Y F, Hua C, You J, Wang X P, Liu X C, Yang G B, Wang C L. Resistance evaluation of potato cultivars and germplasms to Meloidogyne hapla in Heilongjiang Province. Acta Agron Sin, 2017,43:1864-1869 (in Chinese with English abstract).
[9] 王晓强, 崔华栋, 谢勇, 董莹, 卫环宇, 王扬. 危害马铃薯块茎的根结线虫种类鉴定. 见: 中国植物病理学会病原线虫专业委员会主编. 第十二届全国植物线虫学术研讨会论文集. 中国植物病理学会病原线虫专业委员会: 中国植物病理学会, 2014. pp 105-110.
Wang X Q, Cui H D, Xie Y, Dong Y, Wei H Y, Wang Y. Species identification of root knot nematodes on potato tubers. In: Special Committee of Pathogenic Nematodes of Chinese society of Plant Pathology, eds. Proceedings of the 12th National Symposium on Plant Nematodes. Special Committee of pathogenic nematodes of Chinese society for Plant Pathology: Chinese Society for Plant Pathology, 2014. pp 105-110(in Chinese).
[10] 钟伟荣, 郑国组, 朱建军. 水旱轮作防治茄子青枯病. 上海蔬菜, 2000, (1):44.
Zhong W R, Zheng G Z, Zhu J J. Control of eggplant bacterial wilt by crop rotation. Shanghai Vegetables, 2000, (1):44 (in Chinese).
[11] 李泽森, 何卫蓉, 彭建波, 钟艳红, 蔡道辉, 黄增富, 向富勇, 杨顺华. 水旱轮作对龙山百合病虫草害的控制效应. 现代园艺, 2013, (18):9-10.
Li Z S, He W R, Peng J B, Zhong Y H, Cai D H, Huang Z F, Xiang F Y, Yang S H. Effects of flood and drought rotation on control of Lily Disease, insect infestation and grass infestation in Longshan. Modern Hortic, 2013, (18):9-10 (in Chinese).
[12] 曾光辉, 王法格. 单季稻——大棚草莓水旱轮作栽培技术. 现代园艺, 2010, (5):30-31.
Zeng G H, Wang F G. Single cropping rice—cultivation techniques of greenhouses strawberry crop rotation. Modern Hortic, 2010, (5):30-31 (in Chinese).
[13] 王海霞, 殷俊, 凌须美, 丁玉祥, 何静. 水旱轮作对设施番茄生长影响的初探. 农业装备技术, 2011,37(4):43-44.
Wang H X, Yan J, Ling X M, Ding Y X, He J. Study on the effect of crop rotation between flood and drought on tomato growth. Agric Equip Technol, 2011,37(4):43-44 (in Chinese).
[14] 杨云峰, 王伟男, 王克安, 谭德云, 吕晓惠, 张宝贤, 孙建磊, 刘红光. 蓖麻粕防治番茄根结线虫病探讨. 农业科技通讯, 2017, (1):41-43.
Yang Y F, Wang W N, Wang K A, Tan D Y, Lyu X H, Zhang B X, Sun J L, Liu H G. Study on prevention and treatment of tomato root knot nematode by castor meal. Agric Sci Technol Commun, 2017, (1):41-43 (in Chinese).
[15] 烟草品种抗病性鉴定. 中华人民共和国烟草行业标准, YC/T41-1996. p 2.
Identification of Disease Resistance of Tobacco Varieties. Tobacco Industry Standard of the People's Republic of China, YC/T41-1996. p 2 (in Chinese).
[16] 鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2000. pp 22-24.
Bao S D. Agrochemical Analysis of Soil. Beijing: China Agriculture Press, 2000. pp 22-24(in Chinese).
[17] 刘维志. 植物病原线虫学. 北京: 中国农业出版社, 2000. p 379.
Liu W Z. Plant Pathogen Nematology. Beijing: China Agriculture Press, 2000. p 379 (in Chinese).
[18] 吴杨, 周会, 潘大仁. 甘蔗线虫病抗性基因的PCR检测研究. 作物学报, 2006,32:939-942.
Wu Y, Zhou H, Pan D R. PCR detection of nematode resistant gene in Sugarcane. Acta Agron Sin, 2006,32:939-942 (in Chinese with English abstract).
[19] 李梅云, 焦芳婵, 曾建敏, 王丙武, 宋中邦, 吴兴富, 李永平, 高玉龙, 李文正. 不同类型烟草新品种(系)对南方根结线虫的抗性鉴定. 烟草科技, 2017,50(11):22-26.
Li M Y, Jiao F C, Zeng J M, Wang B W, Song Z B, Wu X F, Li Y P, Gao Y L, Li W Z. Characterization of Meloidogyne incognita resistance in new tobacco varieties (lines). Tob Sci Technol, 2017,50(11):22-26 (in Chinese with English abstract).
[20] 席先梅, 白全江, 李玉民. 不同番茄品种抗根结线虫评价. 北方农业学报, 2016,44(4):50-52.
Xi X M, Bai Q J, Li Y M. Evaluation of tomato resistance to root knot nematodes. J North Agric, 2016,44(4):50-52 (in Chinese with English abstract).
[21] 孔祥义, 陈绵才. 根结线虫病防治研究进展. 热带农业科学, 2006,26(2):83-88.
Kong X Y, Chen M C. A review of control of root knot nematodes disease. Chin J Trop Agric, 2006,26(2):83-88 (in Chinese with English abstract).
[22] 计路芬. 白萝卜根结线虫的防治办法. 农业开发与装备, 2019, (5):219.
Ji L F. Control method of root knot nematode of white radish. Agric Develop Equip, 2019, (5):219 (in Chinese).
[23] 高忠奎, 蒋菁, 唐秀梅, 刘菁, 钟瑞春, 韩柱强, 熊发前, 黄志鹏, 吴海宁, 李战, 贺梁琼, 唐荣华. 水旱轮作条件下花生品种筛选及土壤特性变化分析. 南方农业学报, 2018,49:2403-2409.
Gao Z K, Jiang J, Tang X M, Liu J, Zhong R C, Han Z Q, Xiong F Q, Huang Z P, Wu H N, Li Z, He L Q, Tang R H. Peanut varieties selection and soil properties changes under paddy-upland rotation. J Southern Agric, 2018,49:2403-2409 (in Chinese with English abstract).
[24] 刘海霞. 大棚番茄根结线虫病的发病原因及防治措施. 植物医生, 2011,24(3):10-11.
Liu H X. The causes and control measures of tomato root knot nematode in greenhouse. Plant Doctor, 2011,24(3):10-11 (in Chinese).
[25] 张传功, 张吉民, 王璐, 刘清瑞. 番茄根结线虫病的发生特点与综合防治. 种业导刊, 2014, (11):20-21.
Zhang C G, Zhang J M, Wang L, Liu Q R. Occurrence characteristics and comprehensive control of tomato root knot nematode. J Seed Ind Guide, 2014, (11):20-21 (in Chinese).
[26] 肖克, 唐静, 李继福, 邹家龙, 朱建强. 长期水稻-冬油菜轮作模式下钾肥的适宜用量. 作物学报, 2017,43:1226-1233.
doi: 10.3724/SP.J.1006.2017.01226
Xiao K, Tang J, Li J F, Zou J L, Zhu J Q. Optimum amount of potassium fertilizer applied under continuous rice-rapeseed rotation. Acta Agron Sin, 2017,43:1226-1233 (in Chinese with English abstract).
[27] 刘维志. 植物病原线虫学. 北京: 中国农业出版社, 2000. pp 93-96.
Liu W Z. Plant Pathogen Nematology. Beijing: China Agriculture Press, 2000. pp 93-96(in Chinese).
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