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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (9): 1456-1463.doi: 10.3724/SP.J.1006.2020.94191

• RESEARCH NOTES • Previous Articles    

Control effects of different potato varieties (lines) and rice-potato rotation system on root-knot nematode

WU Chao(), LIU Xian-Wen, ZHANG Wei, WANG Qiong*(), GUO Hua-Chun*()   

  1. Tuber and Root Crops Research Institute, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China
  • Received:2019-12-09 Accepted:2020-04-15 Online:2020-09-12 Published:2020-05-08
  • Contact: Qiong WANG,Hua-Chun GUO E-mail:1371757245@qq.com;468050753@qq.com;ynghc@126.com
  • Supported by:
    China Agriculture Research System (Potato, CARS-09-15P).

Abstract:

Root-knot nematode disease, induced by Meloidogyne incognita and Meloidogyne javanica, is one of the main reasons of crop failure in potato continuous cropping in Yunnan province. Variety resistance evaluation, rice-potato rotation model and dynamic changes were studied in order to assess the protect-and-control treatment of potato root-knot nematode disease. Variety resistance evaluation, rice-potato rotation model and dynamic changes were all studied in order to evaluate the protect-and-control treatment of potato root-knot nematode disease. The results from the variety resistance evaluation indicated that among the 18 potato cultivars (lines) no resistant materials were screened resistant to both Meloidogyne incognita and Meloidogyne javanica, whereas the 1002-1 line and Lishu 6 displayed better disease resistance, Qingshu 9 and Cooperation 88 were more sensitive to potato root-knot nematode disease. All kinds of the egg, larva and imago of root-knot nematodes were simultaneously detected in soil with the root-knot nematodes disease. However, after rice-potato rotation, the egg, larva and imago of root-knot nematodes were to disappear entirely in soil, and the root knot nematode disease was no longer occurred in the following potato planting process. Furthermore, the tuber yield per plant of Qingshu 9, Cooperation 88 and Lishu 6 compared with control were significantly different increased by 50.5%, 43.7%, and 26.0%, respectively. These results suggested rice-potato rotation could be an effective way to control potato root knot nematode disease. In addition, to further analyzed the mechanism of rice-potato rotation inhibiting root-knot nematode survival, we observed the dynamic changes of the amount on root knot nematodes in the case of soil flooding. The results showed that no different states of the insect state were detected until submerged more than 60 days. Therefore, we speculated prolonged soil flooding may be the main reason for inhibiting the growth of root-knot nematodes. It is a sustainable and effective method to control potato root-knot nematode by selecting rice-potato rotation with disease-resistant varieties.

Key words: potatoes, varieties (lines), rice-potato rotation cropping, root-knot nematode, control effect

Table 1

Root-knot nematode base per gram of soil (strip g-1)"

样品编号
Number of samples
雌虫
Female worms
幼虫和雄虫
Larvae and male worms

Eggs
总数
Total
土样1 Soil samples 1 0.5±0.1 2.2±0.9 3.0±0.4 5.7±0.6
土样2 Soil samples 2 0.8±0.3 1.8±0.4 2.7±0.4 5.3±0.4
土样3 Soil samples 3 0.5±0 1.3±0.2 2.2±0.2 4.0±0.2

Table 2

Identification results of resistance to root-knot nematodes from two different potato varieties (lines)"

品种(系)
Variety
(lines)
线虫类型
Type of
Meloidogyne
spp.
根系Root 块茎Tuber
感病率
Susceptibility
rate (%)
病情指数
Susceptible
index
抗性
Resistance
level
感病率
Susceptibility
rate (%)
病情指数
Susceptible
index
抗性
Resistance
level
1002-1 M.i 0 0 IM 0 0 IM
M.j 11.11 16.67 HR 2.40 1.60 HR
CIP 81 M.i 44.44 37.04 MR 3.40 2.49 HR
M.j 0 0 IM 0 0 IM
CIP 88 M.i 40 27.50 MR 3.68 3.07 HR
M.j 0 0 IM 0 0 IM
CIP 164 M.i 30 26.67 MR 3.42 3.08 HR
M.j 0 0 IM 0 0 IM
丽薯6号 M.i 20 20 HR 4.35 4.35 HR
Lishu 6 M.j 0 0 IM 0 0 IM
CIP 50 M.i 40 33.33 MR 10.19 5.09 HR
M.j 0 0 IM 0 0 IM
CIP 58 M.i 50 43.33 MR 15.22 9.06 HR
M.j 0 0 IM 0 0 IM
CIP 57 M.i 50 36.67 MR 10.10 9.60 HR
M.j 0 0 IM 0 0 IM
合作88 M.i 71.43 61.90 MS 22.09 12.79 HR
Cooperation 88 M.j 0 0 IM 0 0 IM
CIP 64 M.i 90 67.50 MS 33.88 21.21 HR
M.j 0 0 IM 0 0 IM
青薯9号 M.i 90 66.67 MS 36.54 22.60 HR
Qingshu 9 M.j 30 26.67 MR 8.91 7.92 HR
西海 35
Xihai 35
M.i 80 62.50 MS 27.55 25.00 HR
M.j 0 0 IM 0 0 IM
2013-16D1 M.i 60 60 MS 29.63 27.78 MR
M.j 0 0 IM 0 0 IM
中薯20号 M.i 100 73.33 MS 42.31 28.85 MR
Zhongshu 20 M.j 40 30 MR 7.69 7.69 HR
3S-24 M.i 100 85.00 HS 41.77 29.11 MR
M.j 0 0 IM 0 0 IM
中薯18号 M.i 100 88.89 HS 51.39 33.10 MR
Zhongshu 18 M.j 80 70 HS 6.56 8.20 HR
滇薯701 M.i 70 55.00 MS 39.53 38.37 MR
Dianshu 701 M.j 0 0 IM 0 0 IM
滇同1号 M.i 100 83.33 HS 42.86 39.29 MR
Diantong 1 M.j 0 0 IM 0 0 IM

Table 3

Statistics of root-knot nematodes in tubers, roots, and soil after crop rotation"

作物
Crops
块茎线虫
Nematodes
of tuber
根系线虫
Nematodes
of root
土壤线虫
Nematodes of soil
相对防治效果
Relative control
effect (%)
一茬 青薯9号-青薯9号 Qingshu 9-Qingshu 9 + + +
First 滇杂36-青薯9号 Yunnan miscellaneous 36-Qingshu 9 - - 100
crop 合作88-合作88 Cooperation 88-Cooperation 88 + + +
Apr., 2017 滇杂36-合作88 Yunnan miscellaneous 36-Cooperation 88 - - 100
丽薯6号-丽薯6号 Lishu 6-Lishu 6 + + +
滇杂36-丽薯6号 Yunnan miscellaneous 36-Lishu 6 - - 100
1002-1-1002-1 + + +
滇杂36-1002-1 Yunnan miscellaneous 36-1002-1 - - 100
二茬 青薯9号-青薯9号 Qingshu 9-Qingshu 9 + + +
Second 滇杂36-青薯9号 Yunnan miscellaneous 36 -Qingshu 9 - - - 100
crop 合作88-合作88 Cooperation 88-Cooperation 88 + + +
Nov., 2011 滇杂36-作88 Yunnan miscellaneous 36-Cooperation 88 - - - 100
丽薯6号-丽薯6号 Lishu 6-Lishu 6 + + +
滇杂36-丽薯6号 Yunnan miscellaneous 36-Lishu 6 - - - 100
1002-1-1002-1 + + +
滇杂36-1002-1 Yunnan miscellaneous 36-1002-1 - - - 100
三茬 青薯9号-青薯9号 Qingshu 9-Qingshu 9 + + +
Third 滇杂36-青薯9号 Yunnan miscellaneous 36-Qingshu 9 - - 100
crop 合作88-合作88 Cooperation 88-Cooperation 88 + + +
Apr., 2018 滇杂36-合作88 Yunnan miscellaneous 36-Cooperation 88 - - 100
丽薯6号-丽薯6号 Lishu 6-Lishu 6 + + +
滇杂36-丽薯6号 Yunnan miscellaneous 36-Lishu 6 - - 100
1002-1-1002-1 + + +
滇杂36-1002-1 Yunnan miscellaneous 36-1002-1 - - 100
四茬 青薯9号-青薯9号 Qingshu 9-Qingshu 9 + + +
The 滇杂36-青薯9号 Yunnan miscellaneous 36-Qingshu 9 - - - 100
fourth 合作88-合作88 Cooperation 88-Cooperation 88 + + +
crop 滇杂36-合作88 Yunnan miscellaneous 36-Cooperation 88 - - - 100
Nov., 2018 丽薯6号-丽薯6号 Lishu 6-Lishu 6 + + +
滇杂36-丽薯6号 Yunnan miscellaneous 36-Lishu 6 - - - 100
1002-1-1002-1 + + +
滇杂36-1002-1 Yunnan miscellaneous 36-1002-1 - - - 100

Fig. 1

Effects on yield per plant, effective yield per plant, number of tubers per plant and infection rate of tuber in irrigation and drought rotation A: effects of yield level per plant from irrigation and drought rotation; B: effects of effective yield level per plant from irrigation and drought rotation; C: effects of number of tubers per plant from irrigation and drought rotation; D: effects of infection rate of tuber from irrigation and drought rotation. Q9: Qingshu 9; H88: Cooperation 88; L6: Lishu 6. Different lowercase letters indicate significantly different among treatments at P < 0.05. The results are the averages of the second and fourth crops."

Fig. 2

Dynamic variation of density of root-knot nematodes with immersion time in soil Density number ± number: the base density of root knot nematod."

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