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Preparation of Bacillus velezensis G-1·lipopeptide suspension seed coating agent and its anti-infection effect on Sphacelotheca reiliana

SUN Mei-Hong1,WANG Cai-Qin1,WANG Rong1,BAI Wen-Bin2, GAO Zhen-Feng1,*   

  1. 1Shanxi Agricultural University, Taiyuan 030031, Shanxi, China; 2 Sorghum Research Institute, Shanxi Agricultural University, Yuci 030600, Shanxi, China
  • Received:2023-10-26 Revised:2024-01-30 Accepted:2024-01-30 Published:2024-02-20
  • Supported by:
    This study was supported by the Central Leading Local Science and Technology Development Fund Project (YDZJSX20231C012) and the Shanxi Province Modern Agricultural Miscellaneous Grain Industry Technology System (2023CYJSTX03-16).

Abstract:

In order to screen for biocontrol microorganisms and natural products effective against Sphacelotheca reiliana infection in sorghum root tissue, and to develop a microbial suspension seed coating agent suitable for practical application in sorghum cultivation. In this study, Jinza 2001 sorghum and three excellent disease-resistant bacteria and natural product of lipopeptide were used as the experimental material. Firstly, the anti-infection effects and optimal concentrations of different disease-resistant bacteria and lipopeptide against S. reiliana were determined involving seed germination and in vivo infection rate determination. Subsequently, the optimal formulation, dosage, and storage stability of the suspension seed coating agent were studied based on the active ingredients of the optimum strain and lipopeptide. The anti-infection effect of suspended seed coating agent against S. reiliana was measured under potted conditions. Results showed that Bacillus velezensis G-1 and its secondary metabolites (lipopeptide) exhibited significant efficacy against S. reiliana on infection in sorghum root tissue, with optimal concentrations of 1.0×109 cfu mL-1 for B. velezensis G-1 and and 600 μg mL-1 lipopeptide, respectively. Moreover, there was no significant inhibitory effect on the germination of Jinza 2001 at the optimal concentrations of B. velezensis G-1 and lipopeptide (P > 0.05). The optimal formula for the  B. velezensis G-1·lipopeptide suspension seed coating agent comprised of 5 mL of strain G-1 (1.0 × 1011 cfu mL-1), 5 mL of  lipopeptide substance (8 mg mL-1), 4 g of sodium carboxymethyl cellulose, 0.6 g of sodium alginate, 4 g of dispersant MF, 0.2 g of ferrous sulfate, 1 g of magnesium sulfate, 0.5 g of potassium dihydrogen phosphate, 4 g of color paste, and water added to reach 100 g. The optimal dosage of B. velezensis G-1·lipopeptide suspension seed coating agent was 25 g packaged with 1 kg sorghum seeds, resulting in the anti-infection effect for S. reiliana infecting sorghum root was 87.74% under pot test conditions. Additionally, the B. velezensis G-1·lipopeptide suspension seed coating agent demonstrated a positive promoting effect on the growth of sorghum seedlings and exhibited good storage stability. After 12 months of storage in dark and room temperature conditions, the anti-infection effect of B. velezensis G-1·lipopeptide suspension seed coating agent only decreased by 1.63%. In conclusion, B. velezensis G-1·lipopeptide suspension seed coating agent holds promising application prospects in the development of green prevention and control technology for sorghum head smut.

Key words: 1Shanxi Agricultural University, Taiyuan 030031, Shanxi, China, 2 Sorghum Research Institute, Shanxi Agricultural University, Yuci 030600, Shanxi, China

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