Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica

   

Comparison of wound healing capacity of tubers of different potato varieties

YIN Li-Na, ZHANG Rui,CHEN Guo-Huan,BAI Lei, LI Jun,GUO Hua-Chun,YANG Fang*   

  1. College of Agriculture and Biotechnology, Yunnan Agricultural University / Potato Crops Research Institute, Kunming 650201, Yunnan, China
  • Received:2025-02-26 Revised:2025-06-01 Accepted:2025-06-01 Published:2025-06-10
  • Supported by:
    This study was supported by the China Agriculture Research System of MOF and MARA (CARS-09-P15), the National Key Research and Development Program of China (2022YFD1601802), and the National Natural Science Foundation of China (31660355).

PDF

1

Abstract:

The wound healing ability of potato tubers varies among cultivars. In this study, five tetraploid potato cultivars “Dianshu 47” (D47), “Dianshu 14023” (14023), “Dianshu 1208” (1208), “Cooperation-88” (C88), and “Qingshu 9” (Q9) were used, as experimental materials. The accumulation of suberin and lignin at wound sites was observed, along with the activity of key enzymes and metabolite contents involved in the phenylpropanoid pathway, as well as the relative expression levels of genes related to suberin biosynthesis. The wound healing capacity of the five cultivars was comprehensively evaluated using the membership function method. The results showed that the activities of PAL, 4CL, C4H, and POD, as well as the relative expression levels of StPAL, StPOD, St4CL, and StC4H, increased rapidly during the early stage of wound healing (0–2 d) and peaked during the middle to late stages (4–8 d). As healing progressed, the contents of lignin, flavonoids, and total phenolics increased, while SPP and SPA were rapidly deposited to form the wound periderm. Among the whole cultivars, D47 exhibited, the lowest weight loss rate, 1208 and C88 showed the highest. The deposition rates of SPA, SPP, and lignin layers in D47 were relatively rapid, reaching maximum levels at 6 days post-injury, while deposition in 14023 and 1208 was comparatively slower. Additionally, D47 and C88 showed higher activities of PAL, POD, and C4H, and therelative expression levels of StPAL, StPOD, and StC4H were significantly higher in D47 and Q9 than in the other cultivars (P < 0.05), In contrast, 14023 and 1208 exhibited lower PAL and POD activities. During the wound-healing process, lignin, total phenolics, and flavonoid contents were consistently higher in D47 and lower in 1208 and 14023. Principal component analysis of 18 wound-healing indicators across the 0–8 day period identified weight loss rate, SPA cell layer thickness, SPP cell layer thickness, lignin layer thickness, and the relative expression levels of StPAL, StC4H, and StPOD as representative indicators for evaluating wound healing ability. Based on membership function analysis, the overall ranking of wound healing ability was: D47 (3.9808) > Q9 (3.5767) > C88 (3.4663) > 1208 (3.3546) > 14023 (2.0241). These findings suggest that the phenylpropanoid metabolic pathway plays a key role in the wound suberization process of potato tubers.

Key words: potato varieties, wound healing, phenylpropane metabolism, principal component analysis, membership function

[1] 张志鹏, 谭芸秀, 李宝军, 李永才, 毕阳, 李守强, 王小晶, 张宇, 胡丹. 采后外源脱落酸处理对雾培微型马铃薯周皮木栓化的影响及其机理. 中国农业科学, 2023, 56: 1154–1167.

Zhang Z P, Tan Y X, Li B J, Li Y C, Bi Y, Li S Q, Wang X J, Zhang Y, Hu D. Effects of exogenous abscisic acid treatment on periderm suberification of postharvest mini-tuber potato from aeroponic system and its possible mechanisms. Sci Agrica Sin, 2023, 56: 1154–1167 (in Chinese with English abstract).

[2] 姜红, 王毅, 王斌, 张静荣, 郑晓渊, 毕阳. 马铃薯‘青薯168’块茎浅层和深层损伤愈伤能力的比较. 园艺学报, 2018, 45: 908–918. Jiang H, Wang Y, Wang B, Zhang J R, Zheng X Y, Bi Y. A comparison study of healing ability of shallow and deep wounds in potato tubers ‘Qingshu 168’. Acta Hortic Sin, 2018, 45: 908–918 (in Chinese with English abstract).

[3] Lulai E C, Corsini D L. Differential deposition of suberin phenolic and aliphatic domains and their roles in resistance to infection during potato tuber (Solanum tuberosum L.) wound-healing. Physiol Mol Plant Pathol, 1998, 53: 209–222.

[4] Sabba R P, Lulai E C. Immunocytological analysis of potato tuber periderm and changes in pectin and extensin epitopes associated with periderm maturation. Jashs, 2005, 130: 936–942.

[5] Sabba R P, Lulai E C. Histological analysis of the maturation of native and wound periderm in potato (Solanum tuberosum L.) tuber. Ann Bot, 2002, 90: 1–10.

[6] Wahrenburg Z, Benesch E, Lowe C, Jimenez J, Vulavala V K R, Lyu S Y, Hammerschmidt R, Douches D, Yim W C, Santos P, et al. Transcriptional regulation of wound suberin deposition in potato cultivars with differential wound healing capacity. Plant J, 2021, 107: 77–99.

[7] Zhang X J, Yang R R, Zheng X Y, Wang Q H, Silvy E M, Li Y C, Han Y, Bi Y, Prusky D. UV-C irradiation accelerated the deposition of suberin and lignin at wounds by activating phenylpropanoid metabolism in potato tubers. Sci Hortic, 2023, 309: 111634.

[8] Zhou F H, Jiang A L, Feng K, Gu S T, Xu D Y, Hu W Z. Effect of methyl jasmonate on wound healing and resistance in fresh-cut potato cubes. Postharvest Biol Technol, 2019, 157: 110958.

[9] 张梅花, 杨芳, 马露, 宋嘉驰, 申亚桐. 外源NO和ABA处理对马铃薯块茎创伤周皮中软木脂积累的影响. 西南农业学报, 2021, 34: 1201–1207.

Zhang M H, Yang F, Ma L, Song J C, Shen Y T. Effects of exogenous NO and ABA treatments on accumulation of suberin in wound-healing periderm of potato tubers. Southwest China J Agric Sci, 2021, 34: 1201–1207 (in Chinese with English abstract).

[10] Zheng X Y, Jiang H, Bi Y, Wang B, Wang T L, Li Y C, Gong D, Wei Y N, Li Z C, Prusky D. Comparison of wound healing abilities of four major cultivars of potato tubers in China. Postharvest Biol Technol, 2020, 164: 111167.

[11] 姜红, 毕阳, 李昌健, 王毅, 李生娥, 刘耀娜, 王斌. 马铃薯品种‘青薯168’和‘陇薯3号’块茎愈伤能力的比较. 中国农业科学, 2017, 50: 774–782.

Jiang H, Bi Y, Li C J, Wang Y, Li S E, Liu Y N, Wang B. Comparison of healing ability on potato tuber cultivars ‘Qingshu No.168’ and ‘Longshu No.3’. Sci Agric Sin, 2017, 50: 774–782 (in Chinese with English abstract).

[12] Jin L Q, Cai Q, Huang W L, Dastmalchi K, Rigau J, Molinas M, Figueras M, Serra O, Stark R E. Potato native and wound periderms are differently affected by down-regulation of FHT, a suberin feruloyl transferase. Phytochemistry, 2018, 147: 30–48.

[13] Pollard M, Beisson F, Li Y H, Ohlrogge J B. Building lipid barriers: biosynthesis of cutin and suberin. Trends Plant Sci, 2008, 13: 236–246.

[14] 谢凤, 郝乐, 王振杰, 贾晋, 邵玉涛. 苯丙烷代谢途径分支对生物胁迫响应的研究进展. 中国植保导刊, 2023, 43(2): 23–30.

Xie F, Hao L, Wang Z J, Jia J, Shao Y T. Advances in understanding how branches of the phenylpropanoid pathway respond to biotic stress. China Plant Prot, 2023, 43(2): 23–30 (in Chinese with English abstract).

[15] 柴秀伟, 孔蕊, 李宝军, 朱亚同, 毕阳, Dov Prusky. 一氧化氮对苹果果实愈伤苯丙烷代谢的影响及生理机制分析. 西北植物学报, 2022, 42: 619–627.

Chai X W, Kong R, Li B J, Zhu Y T, Bi Y, Prusky D. Effect of nitric oxide on phenylpropanoid metabolism in healing of apple fruit and analysis of its physiological mechanism. Acta Bot Boreali-Occident Sin, 2022, 42: 619–627 (in Chinese with English abstract).

[16] 韩占红, 王斌, 杨瑞瑞, 杨乾, 李志程, Prusky D. 毕阳. 一氧化氮处理对马铃薯采后块茎愈伤的促进及机制. 食品科学, 2020, 41(21): 222–229.

Han Z H, Wang B, Yang R R, Yang Q, Li Z C, Prusky D, Bi Y. Effect and mechanism of postharvest nitric oxide treatment on promoting wound healing in potato tubers. Food Sci, 2020, 41(21): 222–229 (in Chinese with English abstract).

[17] 邢媛, 李凯峰, 周进华, 郭华春, 李俊. 不同马铃薯品种(系)耐旱性综合评价及其指标筛选. 生态学杂志, 2024, 43: 178–187.

Xing Y, Li K F, Zhou J H, Guo H C, Li J. Comprehensive evaluation of drought tolerance of different cultivars (lines) of potatoes and screening of the indicators. Chin J Ecol, 2024, 43: 178–187 (in Chinese with English abstract).

[18] Kolattukudy P E. Biopolyester membranes of plants: cutin and suberin. Science, 1980, 208: 990–1000.

[19] Jiang H, Wang B, Ma L, Zheng X Y, Gong D, Xue H L, Bi Y, Wang Y, Zhang Z, Prusky D. Benzo-(1, 2, 3)-thiadiazole-7-carbothioic acid s-methyl ester (BTH) promotes tuber wound healing of potato by elevation of phenylpropanoid metabolism. Postharvest Biol Technol, 2019, 153: 125–132.

[20] 张梅花, 马露, 杨芳, 郭华春. 外源NO处理对马铃薯块茎创伤木栓化的影响. 分子植物育种, 2021, 19: 3059–3064.

Zhang M H, Ma L, Yang F, Guo H C. Effects of nitric oxide on wound suberization of potato tuber. Mol Plant Breed, 2021, 19: 3059–3064 (in Chinese with English abstract).

[21] Zhu Y T, Zong Y Y, Liang W, Kong R, Gong D, Han Y, Li Y C, Bi Y, Prusky D. Sorbitol immersion accelerates the deposition of suberin polyphenolic and lignin at wounds of potato tubers by activating phenylpropanoid metabolism. Sci Hortic, 2022, 297: 110971.

[22] 杨芳, 李俊, 郭华春. 马铃薯块茎创伤木栓化过程中形态生理指标及内源激素的变化. 植物生理学报, 2017, 53: 824–830.

Yang F, Li J, Guo H C. Changes of morphological and physiological indices and endogenous hormones during potato tuber wound-healing suberization. Plant Physiol J, 2017, 53: 824–830 (in Chinese with English abstract).

[23] Lulai E C, Suttle J C, Pederson S M. Regulatory involvement of abscisic acid in potato tuber wound-healing. J Exp Bot, 2008, 59: 1175–1186.

[24] 王树斌, 全雪丽, 具红光, 朴世领, 吴松权. 膜荚黄芪肉桂酸-4-羟化酶(C4H)基因克隆与序列分析. 延边大学农学学报, 2012, 34(4): 277–281.

Wang S B, Quan X L, Ju H G, Piao S L, Wu S Q. Cloning and sequence analysis of cinnamate acid-4-hydroxylase (C4H) gene from Astragalus membranaceus. J Agric Sci Yanbian Univ, 2012, 34(4): 277–281 (in Chinese with English abstract).

[25] 孟颖, 邢蕾蕾, 曹晓红, 郭光艳, 柴建芳, 秘彩莉. 小麦Ta4CL1基因的克隆及其在促进转基因拟南芥生长和木质素沉积中的功能. 作物学报, 2022, 48: 63–75.

Meng Y, Xing L L, Cao X H, Guo G Y, Chai J F, Bei C L. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants. Acta Agron Sin, 2022, 48: 63–75 (in Chinese with English abstract).

[26] Xie P D, Yang Y Y, Oyom W, Su T T, Tang Y B, Wang Y, Li Y C, Prusky D, Bi Y. Chitooligosaccharide accelerated wound healing in potato tubers by promoting the deposition of suberin polyphenols and lignin at wounds. Plant Physiol Biochem, 2023, 199: 107714.

[27] Han Y, Yang R R, Wang Q H, Wang B, Prusky D. Sodium silicate promotes wound healing by inducing the deposition of suberin polyphenolic and lignin in potato tubers. Front Plant Sci, 2022, 13: 942022.

[28] Liu L Y, Geng P, Jin X Y, Wei X P, Xue J, Wei X B, Zhang L H, Liu M P, Zhang L, Zong W, et al. Wounding induces suberin deposition, relevant gene expressions and changes of endogenous phytohormones in Chinese yam (Dioscorea opposita) tubers. Funct Plant Biol, 2023, 50: 691–700.

[29] 张昌兵, 唐梅玲, 周洋, 张慧丽, 余静菠, 陈仕勇. 6个饲用燕麦品种苗期对低磷胁迫的响应及耐低磷评价. 西南民族大学学报(自然科学版), 2025, 51(1): 1–7.

Zhang C B, Tang M L, Zhou Y, Zhang H L, Yu J B, Chen S Y. Response of seedling growth and root morphology of six forage oat varieties to low phosphorus stress and evaluation of low phosphorus tolerance. J Southwest Minzu Univ (Nat Sci Edn), 2025, 51(1): 1–7 (in Chinese with English abstract).

[30] 王诗音, 李增辉, 吴冰瑞, 李天宇, 庞玉辉, 马指挥, 李家创, 王黎明, 董普辉. PEG胁迫下小麦新品系萌发期抗旱性鉴定. 山东农业科学, 2024, 56(10): 43–48.

Wang S Y, Li Z H, Wu B R, Li T Y, Pang Y H, Ma Z H, Li J C, Wang L M, Dong P H. Drought resistance identification of new wheat lines at germination stage under PEG stress. Shandong Agric Sci, 2024, 56(10): 43–48 (in Chinese with English abstract).

No related articles found!
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd