Abstract:

From 1957 to 1960,colchicine aqueous solution has been used for doubling the number ofchromosomes in wheat-rye F_1 hybrids.The concentrations of colchicine used for treatmentwere 0.04% and 0.05%.Hybrid seedlings were exposed in colchicine solution for 4 days inroom temperature not over 15℃.After the treatment,the seedlings were transplanted ingreenhouse where a condition of high humidity and comparatively low temperature (usuallybelow 10℃) was maintained until the seedlings had recovered from colchicine poi...