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Acta Agron Sin ›› 1986, Vol. 12 ›› Issue (03): 201-204.

• ORIGINAL PAPERS • Previous Articles     Next Articles

THE SINGLE KERNEL EVALUATION TECHNIQUES FOR THE HIGH-LYSINE CORN(Ⅱ)……COOMASSIE BRILLIANT BIUE STAINING METHOD

Wang Yudong   

  1. Institute of Crop Breeding and Cultivation; Chinese Academy of Agricultural Sciences
  • Received:1983-12-16 Revised:1900-01-01 Online:1986-05-12 Published:1986-05-12

Abstract:

The single kernel evaluation techniques for the high-lysine cornshould be both accurate and simple in order to determine the kernels inlarge quantity.Based on the fact that the Coomassie Brilliant Blue canbe bound to the protein quantitatively,the zein extraction solutionsfrom every kernel are applied to a filter paper,then stained with Coom-assie Brilliant Blue G 250 solution.After destaining the spots of diffe-rent color concentration appears on the filter paper.The dark spots arethe lower lysine kernels,...

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