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Acta Agron Sin ›› 1993, Vol. 19 ›› Issue (06): 520-522.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Barley Protoplast Culture:Embryogenic Callus Formation and Albino Plantlet Regeneration

Liu Bao;Wu Qin-sheng;Liu Da-jun   

  1. Cytogenetics Lab., Nanjing Agricultural University,Nanjing 210014
  • Received:1992-03-24 Revised:1992-08-19 Online:1993-11-12 Published:1993-11-12

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Abstract: Friable embryogenic callus was induced from seeds of spring barley cv. Ruche' cul-tured on MS medium with 3 mg/ L 2, 4--D, 0.2mg/L Kinetin, 200mg/L caseinhydrolysate, 300mg/L glutamine and 30g/L sucrose. These calli were used as sourcematerial for liquid culture and stable embryogenic cell suspensio

Key words: Hordeum vulgare L., Embryogenic cell suspension, Protoplast culture, Albino plantlet

[1] CHEN Jin-Xin;ZHANG Guo-Ping;WANG Jun-Mei;CHEN Chong-Hua;ZHOU Ti-Yao. The Effects of Timing of N Application on Barley β-glucanase Activity and Malt Quality [J]. Acta Agron Sin, 2004, 30(01): 47-51.
[2] Xiao Wen-yan; Wang Lian-zheng. Protoplast Culture and Plant Regeneration of Immature Cotyledons of Soybean (Glycine max L.) [J]. Acta Agron Sin, 1994, 20(06): 665-669.
[3] Lu Hui-neng Gai Jun-yi;Ma Yu-hua;Wei Zhi-ming;Xu Zhi-hong. Soybean Protoplast Culture under Different Hormone Conditions and Plantlet Regeneration [J]. Acta Agron Sin, 1993, 19(04): 328-333.
[4] Zhou Hanyang; She Jianming; Lu Weizhong; Wu Heming. Study on Plantlet Regeneration from Protoplasts of Brassicanapus [J]. Acta Agron Sin, 1991, 17(05): 340-345.
[5] Xu Shaoying; Zhang Guoping; Qian Weijun; Huang Jirong. Studies on the Effect of Vernalization in Barley [J]. Acta Agron Sin, 1989, 15(04): 319-327.
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