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Acta Agron Sin ›› 2000, Vol. 26 ›› Issue (02): 143-147.

• ORIGINAL PAPERS • Previous Articles     Next Articles

A Chimeric Antigen Gene of Hepatitis C Virus was Introduced into Chloroplast of Tobacco & the Study of Transgenic Plant Homogenization

SHAN Song;ZHANG Zhong-Lin;WU Xiang-Fu;SHEN Gui-Fang;   

  1. Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081
  • Received:1998-11-30 Revised:1999-06-18 Online:2000-03-12 Published:2000-03-12
  • Contact: SHEN Gui-Fang

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Abstract: Spectinomycin-resistant calli and shoots were obtained from tobacco leaves bombarded with pSS3 DNA which contains the NS3-C chimeric antigen gene of hepatitis C virus. Further molecular screening on the transformed tobacco plants such as Southern blot and PCR amplifying were performed, which indicat

Key words: Hepatitis C virus, Chimeric antigen gene, Chloroplast expression system, Biolistic transformation, Homogenization

[1] XING Li-Ping,QIAN Chen,LI Ming-Hao,CAO Ai-Zhong,WANG Xiu-E,CHEN Pei-Du. Transformation of Antisense Wheat Mlo (Ta-Mlo) Gene and Wheat Powdery Mildew Resistance Analysis of Transgenic Plants [J]. Acta Agron Sin, 2013, 39(03): 431-439.
[2] Shan Song;Zhang Zhonglin;Chen Xi;Liu Chunying;Shen Guifang. Cloning and Modification of 16S Promoter of Tobacco Chloroplast Genome and Production of Transgenic Plants [J]. Acta Agron Sin, 1999, 25(05): 536-540.
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