Abstract:

For the sake of proving the localization character of Bt crystal protein in transgenic Bt cotton cell and illustrating the insect resistance stability of Bt cotton, the study on the sub-cellular orientation of the Bt crystal protein in transgenic Bt cotton cell was conducted by means of immunocyte localization. The results showed that the Bt crystal protein were mostly located in cytoplasm and the intercellular space of Bt transgenic cotton GK-12 cotyledon mesophyll cells. The distribution density was larger in cytoplasm than in the intercellular space. In cytoplasm, the crystal protein was mainly distributed on the cell membrane, endoplasmic reticulum, chloroplast and plastid, especially on the surface of chloroplast and plastid with a high density. From above, we know that the Bt. crystal protein is straightly located onto inertial organelle—chloroplast and its plastid.

Key words: Transgenic Bt cotton, Crystal protein, Sub-cellular orientation