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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 802-808.doi: 10.3724/SP.J.1006.2009.00802

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Localization of MLPK and SSP Genes for Self-Incompatibility of Brassica oleracea by Fluorescence in situ Hybridization

RONG Xiao-Ying1,ZHU Li-Quan1*,WANG Yong1,GAO Qi-Guo2,CHEN Xiao-Dan1,YANG Yang2,WANG Xiao-Jia2   

  1. 1Plant Physiology and Biochemistry Laboratory of Southwest University,Chongqing 400716,China;2Key Laboratory in Olericulture of Chongqing,Southwest University,Chongqing 400716,China
  • Received:2008-10-15 Revised:2009-01-20 Online:2009-05-12 Published:2009-03-23
  • Contact: ZHU Li-Quan,023-68250794 E-mail:zhuliquan@swu.edu.cn

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Abstract:

In Brassica, self-incompatibility recognition is controlled by the multiallelic gene complex (S-haplotypes) at the S-locus, which encodes both the male determinant S-locus pollen coat protein (SCR/SP11) and the female determinant S-locus receptor kinase (SRK). Studies of MLPK and SSP genes for self-incompatibility of Brassica have been gradually widespread since they were identified. However, the position and copy number of MLPK and SSP genes in Brassica oleracea genome are still unclear. In this paper, the localization of MLPK and SSP genes for self-incompatibility of Brassica oleracea on prometaphase chromosomes, early pachytene chromosomes and extended DNA fibers was conducted successfully by fluorescence in situ hybridization. The results indicated that MLPK probe was hybridized onto the short arm of a pair of homologous prometaphase chromosomes, and the percent distance from centromere to the signal point was about 53.41±3.16; SSP probe was hybridized onto the long arm of a pair of homologous prometaphase chromosomes with the satellite, and the percent distance from centromere to the signal point was about 78.36±4.26. Hybridization signals from three kinds of cytological targets with different FISH resolutions showed that both MLPK gene and SSP gene might be located at a single-copy locus in Brassica oleracea genome. Repeated FISH indicated that both MLPK and 5S rDNA probes were hybridized onto the same chromosomes. According to karyotype standard of Armstrong, it was primarily inferred that MLPK gene was located on the chromosome 2, and SSP gene on the chromosome 7. The results presumably revealed thatneither MLPK nor SSP is linked to S-locus. And they locate respectively on the different chromosomes in Brassica oleracea. In addition, the collinearity relationship of MLPK as well as SSP between Brassica and Arabidopsis thaliana was also discussed on the basis of comparative genomics.

Key words: Brassica oleracea, MLPK gene, SSP gene, Fluorescence in situ hybridization, Self-incompatibility


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