Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 802-808.doi: 10.3724/SP.J.1006.2009.00802

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Localization of MLPK and SSP Genes for Self-Incompatibility of Brassica oleracea by Fluorescence in situ Hybridization

RONG Xiao-Ying1,ZHU Li-Quan1*,WANG Yong1,GAO Qi-Guo2,CHEN Xiao-Dan1,YANG Yang2,WANG Xiao-Jia2   

  1. 1Plant Physiology and Biochemistry Laboratory of Southwest University,Chongqing 400716,China;2Key Laboratory in Olericulture of Chongqing,Southwest University,Chongqing 400716,China
  • Received:2008-10-15 Revised:2009-01-20 Online:2009-05-12 Published:2009-03-23
  • Contact: ZHU Li-Quan,023-68250794 E-mail:zhuliquan@swu.edu.cn

PDF

1629

Cited

4

Abstract:

In Brassica, self-incompatibility recognition is controlled by the multiallelic gene complex (S-haplotypes) at the S-locus, which encodes both the male determinant S-locus pollen coat protein (SCR/SP11) and the female determinant S-locus receptor kinase (SRK). Studies of MLPK and SSP genes for self-incompatibility of Brassica have been gradually widespread since they were identified. However, the position and copy number of MLPK and SSP genes in Brassica oleracea genome are still unclear. In this paper, the localization of MLPK and SSP genes for self-incompatibility of Brassica oleracea on prometaphase chromosomes, early pachytene chromosomes and extended DNA fibers was conducted successfully by fluorescence in situ hybridization. The results indicated that MLPK probe was hybridized onto the short arm of a pair of homologous prometaphase chromosomes, and the percent distance from centromere to the signal point was about 53.41±3.16; SSP probe was hybridized onto the long arm of a pair of homologous prometaphase chromosomes with the satellite, and the percent distance from centromere to the signal point was about 78.36±4.26. Hybridization signals from three kinds of cytological targets with different FISH resolutions showed that both MLPK gene and SSP gene might be located at a single-copy locus in Brassica oleracea genome. Repeated FISH indicated that both MLPK and 5S rDNA probes were hybridized onto the same chromosomes. According to karyotype standard of Armstrong, it was primarily inferred that MLPK gene was located on the chromosome 2, and SSP gene on the chromosome 7. The results presumably revealed thatneither MLPK nor SSP is linked to S-locus. And they locate respectively on the different chromosomes in Brassica oleracea. In addition, the collinearity relationship of MLPK as well as SSP between Brassica and Arabidopsis thaliana was also discussed on the basis of comparative genomics.

Key words: Brassica oleracea, MLPK gene, SSP gene, Fluorescence in situ hybridization, Self-incompatibility


[1] Stein J C, Howlett B, Boyes D C, Nasrallah M E, Nasrallah J B. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc Natl Acad Sci USA, 1991, 88: 8816–8820

[2] Goring D R, Rothstein S J. The S-locus receptor kinase gene in a self-incompatible Brassica napus line encodes a functional serine/threonine kinase. Plant Cell, 1992, 4: 1273–1281

[3] Takayama S, Shiba H, Iwano M, Shimosato H, Che F S, Watanabe M, Suzuki G, Hinata K, Isogai A. The pollen determinant of self-incompatibility in Brassica campestris. Proc Natl Acad Sci USA, 2000, 97: 1920–1925

[4] Boyes D C, Nasrallah M E, Vrebalov J, Nasrallah J B. The self-incompatibility (S) haplotypes of Brassica contain highly divergent and rearranged sequences of ancient origin. Plant Cell, 1997, 9: 237–247

[5] Gu T S, Mazzurco M, Sulaman W, Matias D D, Goring D R. Binding of an arm repeat protein to the kinase domain of the S-locus receptor kinase. Proc Natl Acad Sci USA, 1998, 95: 382–387

[6] Cabrillac D, Cock J M, Dumas C, Gaude T. The S-locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins. Nature, 2001, 410: 220–223

[7] Goring D R, Walker J C. Self-rejection — A new kinase connection. Science, 2004, 303: 1474–1475

[8] Murase K, Shiba H, Iwano M, Che F S, Watanabe M, Isogai A, Takayama S. A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling. Science, 2004, 303: 1516–1519

[9] Kakita M, Murase K, Iwano M, Matsumoto T, Watanabe M, Shiba H, Isogai A, Takayama S. Two distinct forms of M-locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce self-incompatibility signaling in Brassica rapa. Plant Cell, 2007, 19: 3961–3973

[10] Wu N-B(吴能表). Study on evocable protein phosphorylation and correlative characters in the self-incompatible of Brassica oleracea L. PhD Dissertation of Southwest Agricultural University, 2003. pp 51–52(in Chinese with English abstract)

[11] Zhao Y-B(赵永斌), ZHU L-Q(朱利泉). Cloning and sequences analysis of SRK substrate protein gene from Brassica oleracea. Acta Agric Boreali-occident Sin (西北农业学报), 2007, 16(6): 102–106(in Chinese with English abstract)

[12] Hasterok R, Wolny E, Hosiawa M, Kowalczyk M, Kulak-Ksiazczyk S, Ksiazczyk T, Heneen W K, Maluszynska J. Comparative analysis of rDNA distribution in chromosomes of various species of Brassicaceae. Ann Bot, 2006, 97: 205–216

[13] Chen R-Y(陈瑞阳), Song W-Q(宋文芹), Li X-L(李秀兰). Wall degradation hypotonic methed of preparing chromosome sample in plant and its significance in the cytogenetics, Acta Genet Sin (遗传学报), 1982, 9(2): 151–159(in Chinese with English abstract)

[14] Zhong X-B(钟筱波), Fransz P F, Wennekes J, Kammen A, De Jong J H, Zabel P. Fluorescence in situ hybridization to pachytene chromosomes and extended DNA fibres in plants. Acta Genet Sin (遗传学报), 1998, 25(2): 142–149(in Chinese with English abstract)

[15] Yang K, Qi H Y, Zhu L Q, Wang X J. Localization of S genes on extended DNA fibers (EDFs) in Brassica oleracea by high-resolution FISH. Acta Genet Sin, 2006, 33(3): 277–284

[16] Zhao Y-B赵永斌), Zhu L-Q(朱利泉), Wang X-J(王小佳). Cloning and sequences analysis of M-locus protein kinase gene from Brassica oleracea. Acta Agron Sin (作物学报), 2006, 32(1): 46–50(in Chinese with English abstract)

[17] Cheng Z K, Buell C R, Wing R A, Jiang J M. Resolution of fluorescence in situ hybridization mapping on rice mitotic prometaphase chromosomes, meiotic pachytene chromosomes and extended DNA fibers. Chrom Res, 2002, 10: 379–387

[18] Armstrong S J, Fransz P, Marshall D F, Jones G H. Physical mapping of DNA repetitive sequences to mitotic and meiotic chromosomes of Brassica oleracea var. alboglabra by fluorescence in situ hybridization. Heredity, 1998, 81: 666–673

[19] Li G, Gao M, Yang B, Quiros C F. Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping. Theor Appl Genet, 2003, 107: 168–180

[20] Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Kondo M, Fujimura M, Nunome T, Fukuoka H, Hirai M, Matsumoto S. Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana: The genetic origin of clubroot resistance. Genetics, 2006, 173: 309–319

[21] Lukens L, Zou F, Lydiate D, Parkin I, Osborn T. Comparison of a Brassica oleracea genetic map with the genome of Arabidopsis thaliana. Genetics, 2003, 164: 359–372

[22] Howell E C, Barker G C, Jones G H, Kearsey M J, King G J, Kop E P, Ryder C D, Teakle G R, Vicente J G, Armstrong S J. Integration of the cytogenetic and genetic linkage maps of Brassica oleracea. Genetics, 2002, 161: 1225–1234

[23] Wang C J R, Harper L, Cande W Z. High-resolution single-copy gene fluorescence in situ hybridization and its use in the construction of a cytogenetic map of maize chromosome 9. Plant Cell, 2006, 18: 529–544

[24] Kamisugi Y, Nakayama S, O’Neil C M, Mathias R J, Trick M, Fukui K. Visualization of the Brassica self-incompatibility S-locus on identified oilseed rape chromosomes. Plant Mol Biol, 1998, 38: 1081–1087

[25] Van De Rijke F M, Florijn R J, Tanke H J, Raap A K. DNA fiber-FISH staining mechanism. J Histochem Cytochem, 2000, 48: 743–745

[26] Stone S L, Anderson E M, Mullen R T, Goring DR. ARC1 is an E3 ubiquitin ligase and promotes the ubiquitination of proteins during the rejection of self-incompatible Brassica pollen. Plant Cell, 2003, 15: 885–898
[1] ZHANG Yi-Zhong, ZENG Wen-Yi, DENG Lin-Qiong, ZHANG He-Cui, LIU Qian-Ying, ZUO Tong-Hong, XIE Qin-Qin, HU Deng-Ke, YUAN Chong-Mo, LIAN Xiao-Ping, ZHU Li-Quan. Codon usage bias analysis of S-locus genes SRK, SLG, and SP11/SCR in Brassica oleracea [J]. Acta Agronomica Sinica, 2022, 48(5): 1152-1168.
[2] XIE Qin-Qin, ZUO Tong-Hong, HU Deng-Ke, LIU Qian-Ying, ZHANG Yi-Zhong, ZHANG He-Cui, ZENG Wen-Yi, YUAN Chong-Mo, ZHU Li-Quan. Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea [J]. Acta Agronomica Sinica, 2022, 48(1): 108-120.
[3] Tong-Hong ZUO, He-Cui ZHANG, Qian-Ying LIU, Xiao-Ping LIAN, Qin-Qin XIE, Deng-Ke HU, Yi-Zhong ZHANG, Yu-Kui WANG, Xiao-Jing BAI, Li-Quan ZHU. Molecular cloning and expression analysis of BoGSTL21 in self-incompatibility Brasscia oleracea [J]. Acta Agronomica Sinica, 2020, 46(12): 1850-1861.
[4] WANG Yu-Kui,ZHANG He-Cui,BAI Xiao-Jing,LIAN Xiao-Ping,SHI Song-Mei,LIU Qian-Ying,ZUO Tong-Hong,ZHU Li-Quan. Characteristics and expression analysis of BoPINs family genes in Brassica oleracea [J]. Acta Agronomica Sinica, 2019, 45(8): 1270-1278.
[5] Xiao-Jing BAI,Xiao-Ping LIAN,Yu-Kui WANG,He-Cui ZHANG,Qian-Ying LIU,Tong- Hong ZUO,Yi-Zhong ZHANG,Qin-Qin XIE,Deng-Ke HU,Xue-Song REN,Jing ZENG,Shao-Lan LUO,Min PU,Li-Quan ZHU. Cloning and analysis of BoCDPK14 in self-incompatibility Brasscia olerace [J]. Acta Agronomica Sinica, 2019, 45(12): 1773-1783.
[6] Shao-Lan LUO,Xiao-Ping LIAN,Min PU,Xiao-Jing BAI,Yu-Kui WANG,Jing ZENG,Song-Mei SHI,He-Cui ZHANG,Li-Quan ZHU. Molecular Cloning, Location and Expression Analysis of Brasscia oleracea Zinc Finger Protein Transcription Factor BoC2H2 [J]. Acta Agronomica Sinica, 2018, 44(11): 1650-1660.
[7] ZHANG Yang,HU Zhong-Ying,ZHAO Yue-Ming,LI Na,XIE Li-Nan. DNA Methylation Dynamic Analysis of Self Compatible Line and Self-Incompatible Line of Brassica oleracea var. acephala at Seed Germination Stage [J]. Acta Agron Sin, 2016, 42(04): 532-539.
[8] GAO Qi-Guo,LIU Yu-Dong,PU Quan-Ming,ZHANG Lin-Cheng,ZHU Li-Quan,WANG Xiao-Jia. Interaction Analysis of Brassica oleracea L. BoExo70A1 with BoSEC3 and BoExo84 Proteins by Using Yeast Two-hybird System [J]. Acta Agron Sin, 2015, 41(06): 972-978.
[9] ZHU Li-Quan,ZHOU Yan. Protein Elements and Signal Transduction Process of Self-Incompatibility in Brassica oleracea [J]. Acta Agron Sin, 2015, 41(01): 1-14.
[10] ZHANG Lin-Cheng,GAO Qi-Guo,PU Quan-Ming,REN Xue-Song,LIU Yu-Dong,ZHU Li-Quan,WANG Xiao-Jia. Cloning and Expression Analysis of BoLH01and BoLH02 Genes in Cabbage [J]. Acta Agron Sin, 2014, 40(08): 1371-1379.
[11] XUE Li-Yan,LUO Bing,ZHU Li-Quan,YANG Yong-Jun,ZHANG He-Cui,CHANG Deng-Long,CHEN Song,PENG Yi-Bo,YANG Hong,ZENG Jing,YANG Kun,GAO Qi-Guo,LI Cheng-Qiong,REN Xue-Song,WANG Xiao-Jia. Identifications of DNA Sequences Encoding Key Region of SCR Interacting with SRK Extracellular Domain by Using Yeast Two-Hybrid System [J]. Acta Agron Sin, 2012, 38(09): 1583-1591.
[12] WEI Jing-Yi,GAO Qi-Guo,REN Xue-Song,WANG Xiao-Jia,LI Cheng-Qiong,SONG Ming. Construction of eSRK Chimeras and Interaction between eSRK Chimeras and SCRs from Brassica oleracea L. [J]. Acta Agron Sin, 2012, 38(09): 1631-1639.
[13] YANG Hun, ZHANG He-Cui, Richard CONVERSE, SHU Li-Quan, YANG Yong-Jun, XUE Li-Yan, LUO Bing, CHANG De-Long, GAO Qi-Guo, WANG Xiao-Jia. Interaction between Two Self-incompatible Signal Elements, EXO70A1 and ARC1 [J]. Acta Agron Sin, 2011, 37(12): 2136-2144.
[14] LUO Bing, XUE Li-Yan, SHU Li-Quan, ZHANG He-Cui, PENG Yi-Bei, CHEN Song, YANG Hong, YANG Hun, LI Cheng-Qiong, WANG Xiao-Jia. Detection of Interactions between SCR and SRK in Brassica oleracea L. by Yeast Two-Hybrid System [J]. Acta Agron Sin, 2011, 37(04): 579-586.
[15] LU Kun,ZHANG Kai,CHAI You-Rong,LU Jun-Xing,TANG Zhang-Lin. Cloning and Comparative Analysis of PURPLE ACID PHOSPHATASE 17 Gene Families in Brassica oleracea and Brassica rapa [J]. Acta Agron Sin, 2010, 36(3): 517-525.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd