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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 848-854.doi: 10.3724/SP.J.1006.2009.00848

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Allelic Composition and Expression of  Vernalization Gene Vrn-1 in Wheat Cultivar Zhengmai 9023

YUAN Xiu-Yun12，LI Yong-Chun1**，MENG Fan-Rong3，YAN Yan-Tao1,YIN Jun1*   

  1. 1National Engineering Research Center for Wheat,Henan Agricultural University,Zhengzhou 450002,China;2Zhengzhou Normal College,Zhengzhou 450044,China;3College of Life Science,Henan Agricultural University,Zhengzhou 450002,China
  • Received:2008-09-11 Revised:2009-02-14 Online:2009-05-12 Published:2009-03-23
  • Contact: YIN Jun E-mail:xmzxyj@126.com

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Abstract:

Zhengmai 9023 is an elite winter wheat (Triticum aestivum L.) cultivar grown in a large scale in China, and often injured by coldness when it easily starts reproductive growth before winter because of its weak vernalization characteristic. Vernalization gene VRN-1 is one of the key genes controlling the conversion from vegetative growth to reproductive growth in wheat. To explore the regulation mechanism of vernalization in Zhengmai 9023, the VRN-1 gene was cloned from leaf tissues using gene-specific PCR amplification technique, and its expressions were analyzed under simulated vernalization at 0–2°C for 0, 10, 20, and 30 d. The gene-specific primers were designed for semiquantitative PCR analysis based on the sequences of the VRN-A1, VRN-B1, and VRN-D1, which were cloned from Zhengmai 9023. The results showed that the genotype of VRN-1 was vrnA1VrnB1vrnD1 with the unique dominant allele in B genome of Zhengmai 9023. Under the treatment of 0 d vernalization, the expressions of VRN-A1 and VRN-D1 were not detected at one-leaf stage, whereas VRN-B1 expressed at a low level and the expressions of the three VRN-1 alleles were all at relative high levels from three-leaf stage to flowering stage. However, under the treatments with 10 to 30 d vernalizaion, the three alleles of VRN-1 gene showed high-level expressions throughout the period from one-leaf to flowering stages.

Key words: Zhengmai 9023, Vernalization gene VRN-1;Gene cloning, Semiquantitative RT-PCR


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