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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (05): 754-763.doi: 10.3724/SP.J.1006.2010.00754

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mining Elite Alleles for Seed Vigor Traits in Two Populations of Japonica Rice (Oryza sativa L.) in Taihu Lake Region

WANG Yang1,2,GUO Yuan1,3,HONG De-Lin1,*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Department of Agricultural Resource and Environment, Heilongjiang University, Harbin 150008, China; 3 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
  • Received:2009-08-22 Revised:2009-12-19 Online:2010-05-12 Published:2010-03-03
  • Contact: HONG De-Lin,E-mail:delinhong@njau.edu.cn

Abstract:

Direct seeding in rice (Oryza sativa L.) is a labor-saving, high efficient and water-saving cultivation pattern. Lower temperature stress is the main factor affecting seedling establishment in direct seeding rice field. Breeding rice cultivars with high seed vigor is one of the ways to improve seedling establishment. In order to provide genetic information and breeding materials for breeding cultivars suitable to direct sowing in japonica rice, we conducted this research. Root length, shoot height, and dry weight of seedlings germinated for 7 days on the sloped board were investigated in a natural population composed of 94 varieties from Taihu Lake Region and a recombinant inbred line (RIL) population derived from a cross between Xiushui 79 and C Bao in japonica rice (Oryza sativa L.). QTL analysis of seed vigor was performed by using General Linear Model program in Tassel software for the natural population and Composite Internal Mapping program in Win QTL Cartographer 2.5 software for the RIL population. Elite alleles and their carrier materials were determined on the basis of the result of QTL analysis. The results obtained were as follows: (1) In the natural population, eleven SSR marker loci associated with seed vigor were detected. Forty-two elite alleles for seed vigor traits were detected in the natural population. Among them, 17 associated with root length, 13 with shoot height and 12 with dry weight. Diantun 502 xuanzao, Yangdao 6, Kaiqing, Xianhui 429 and C Bao were carrier materials for the elite alleles of seed vigor. (2) In the RIL population, nine QTLs, including two for root length, four for shoot height and three for dry weight, were detected. All the elite alleles came from C Bao except RM525-143 bp, which associated with dry weight and came from Xiushui 79. (3) In both populations, SSR marker locus associated with root length was detected on chromosome 1, SSR marker loci associated with shoot height were detected on chromosomes 2, 8 and 11, and SSR marker locus associated with dry weight was detected on chromosome 2. More or better elite alleles were detected in the natural population than in the RIL population.

Key words: Japonica rice(Oryza sativa L.), Seed vigor, Elite alleles, Natural population, Family population

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