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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (12): 1787-1797.doi: 10.3724/SP.J.1006.2016.01787

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Transcription Factor SiNF-YA5 from Foxtail Millet (Setaria italica) Conferred Tolerance to High-salt Stress through ABA-independent Pathway in Transgenic Arabidopsis

HUANGSuo1,**,HULi-Qin1,**,XUDong-Bei1,2,LIWei-Wei1,3,XUZhao-Shi1,LILian-Cheng1,ZHOUYong-Bin1,2,DIAOXian-Min1,JIAGuan-Qing1,MAYou-Zhi1,CHENMing1,*   

  1. 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility For Crop Gene Resource and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crop, Ministry of Agriculture, Beijing 100081, China; 2 College of Agronomy, Northwest A&F University / State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, China; 3 College of Life Science and Technology, Harbin Normal University / Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, Harbin 150025, China
  • Received:2016-03-06 Revised:2016-06-20 Online:2016-12-12 Published:2016-07-04
  • Contact: Chan Ming,E-mail:chenming02@caas.cn,Tel:13683360891
  • Supported by:

    ThisworkwasfundedbytheNationalKeyProjectforResearchonTransgenicBiology(2014ZX08002-002)

Abstract:

Nuclear transcription factor Y (NF-Y) consisting of three subunits, NF-YA, NF-YB, and NF-YC, plays an essential role in many biologic processes, including growth, development, and abiotic stress response. In this study, a NF-Y like transcription factor gene SiNF-YA5 was isolated from foxtail millet variety Longgu 25. The full-length sequence of SiNF-YA5 gene is 924 bp, encoding 307 amino acids. Molecular weight and isoelectric point of SiNF-YA5 protein are 33.76 kD and 9.19, respectively. There is a conserved CBF domain from the 149th to the 210th amino acids of SiNF-YA5. According to the subcellular localization analysis, SiNF-YA5 was mainly localized and expressed on the plasma membrane and nucleus in plant cell. Gene functional analysis showed that under different NaCl concentration treatments, the germination rate of SiNF-YA5 transgenic Arabidopsis was significantly higher than that of wild-type (WT) Arabidopsis during seed germination stage; root surface area and fresh weight of SiNF-YA5 transgenic Arabidopsis remarkably increased compared with WT during seedling stage. Those results indicated that the overexpression of SiNF-YA5 in transgenic plants could enhance tolerance to high salt. Gene expression analysis showed that the expressions of two salt stress related genes, namely NHX1 and LEA7, increased significantly in SiNF-YA5 transgenic plants. On the other hand, there was no obvious difference in sensitivity to ABA between SiNF-YA5 transgenic Arabidopsis and WT showed during seed germination and seedling stages indicating that SiNF-YA5 could enhance salt tolerance through ABA-independent pathway in transgenic plants.

Key words: Foxtail millet (Setaria italic), NF-Y like transcription factor, High salt stress, ABA independent signaling pathway

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