Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2007, Vol. 33 ›› Issue (02): 317-321.

• ORIGINAL PAPERS • Previous Articles     Next Articles

DNA Sampling Strategy and Calculation of Genetic Distance between Maize (Zea mays L.) Populations

HOU Ben-Jun1,WANG Tie-Gu12,CHEN Yan-Hui1*,WU Lian-Cheng1,KU Li-Xia1   

  1. 1 Agronomy College, Henan Agricultural University, Zhengzhou 450002, Henan; 2Ion Beam Biotechnology Key Laboratory, Zhengzhou University, Zhengzhou 450052, Henan, China
  • Received:2006-01-04 Revised:1900-01-01 Online:2007-02-12 Published:2007-02-12
  • Contact: CHEN Yan-Hui

PDF

1183

Cited

3

Abstract:

SSR is a good molecule marker with broad application. Bulk sampling is feasible to analyze genetic diversity of maize population by SSR. It is necessary to study influence of genetic distance as using bulk sampling. In present study, the genetic distances between two populations were compared and analyzed by 50 pairs of SSR primers, checked by inbred lines Huangzao 4 and Mo17, and using 60 individuals DNA samples of Golden Queen and Yuzong 5 maize populations with six treatments (the individual DNA samples, the mixed DNA samples with 5 individuals, 10 individuals, 15 individuals, 20 individuals, and 30 individuals, respectively). The results indicated that using the individual DNA samples can detect the alleles with very low frequencies, and make the detected allele number decreased gradually and the frequency of missing alleles increased with the increase of mixed individuals in the treatmen. The results of the genetic distance between two populations calculated by three models suggested that the genetic distances GD, GD(J) and GD(N) showed a gradually increasing trend with the increasing of the mixed individual number, but the increasing extent of GD(J) and GD(N) was wider. There was an obvious deviation between GD(J), GD(N) from mixed samples and the GD from 60 individual DNA samples based on allele frequency. The results and the comparison of the genetic distances based on different calculation methods suggested that the 10 individuals’ DNA mixture is the best sampling strategy when genetic diversity and polymorphism in maize populations are studied by SSR.
If the Nei’s genetic distance GD(N) and Jaccord’s genetic distance GD(J) are calculated according to 0, 1 data, (GD(N)+GD(J))/2 will be calculated close to the genetic distance by allele frequency of individuals. Gene frequency can be studied by using capillary electrophoresis and SSR fluorescence detection. There will be a broad application prospect about bulk sampling strategy with SSR marker.

Key words: SSR, Maize, Genetic distance, Genetic diversity, Sampling method

[1] XIAO Ying-Ni, YU Yong-Tao, XIE Li-Hua, QI Xi-Tao, LI Chun-Yan, WEN Tian-Xiang, LI Gao-Ke, HU Jian-Guang. Genetic diversity analysis of Chinese fresh corn hybrids using SNP Chips [J]. Acta Agronomica Sinica, 2022, 48(6): 1301-1311.
[2] WANG Dan, ZHOU Bao-Yuan, MA Wei, GE Jun-Zhu, DING Zai-Song, LI Cong-Feng, ZHAO Ming. Characteristics of the annual distribution and utilization of climate resource for double maize cropping system in the middle reaches of Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(6): 1437-1450.
[3] YANG Huan, ZHOU Ying, CHEN Ping, DU Qing, ZHENG Ben-Chuan, PU Tian, WEN Jing, YANG Wen-Yu, YONG Tai-Wen. Effects of nutrient uptake and utilization on yield of maize-legume strip intercropping system [J]. Acta Agronomica Sinica, 2022, 48(6): 1476-1487.
[4] CHEN Jing, REN Bai-Zhao, ZHAO Bin, LIU Peng, ZHANG Ji-Wang. Regulation of leaf-spraying glycine betaine on yield formation and antioxidation of summer maize sowed in different dates [J]. Acta Agronomica Sinica, 2022, 48(6): 1502-1515.
[5] SHAN Lu-Ying, LI Jun, LI Liang, ZHANG Li, WANG Hao-Qian, GAO Jia-Qi, WU Gang, WU Yu-Hua, ZHANG Xiu-Jie. Development of genetically modified maize (Zea mays L.) NK603 matrix reference materials [J]. Acta Agronomica Sinica, 2022, 48(5): 1059-1070.
[6] XU Jing, GAO Jing-Yang, LI Cheng-Cheng, SONG Yun-Xia, DONG Chao-Pei, WANG Zhao, LI Yun-Meng, LUAN Yi-Fan, CHEN Jia-Fa, ZHOU Zi-Jian, WU Jian-Yu. Overexpression of ZmCIPKHT enhances heat tolerance in plant [J]. Acta Agronomica Sinica, 2022, 48(4): 851-859.
[7] LIU Lei, ZHAN Wei-Min, DING Wu-Si, LIU Tong, CUI Lian-Hua, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping. Genetic analysis and molecular characterization of dwarf mutant gad39 in maize [J]. Acta Agronomica Sinica, 2022, 48(4): 886-895.
[8] CHEN Xiao-Hong, LIN Yuan-Xiang, WANG Qian, DING Min, WANG Hai-Gang, CHEN Ling, GAO Zhi-Jun, WANG Rui-Yun, QIAO Zhi-Jun. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR [J]. Acta Agronomica Sinica, 2022, 48(4): 908-919.
[9] ZHANG Xia, YU Zhuo, JIN Xing-Hong, YU Xiao-Xia, LI Jing-Wei, LI Jia-Qi. Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials [J]. Acta Agronomica Sinica, 2022, 48(4): 920-929.
[10] YAN Yu-Ting, SONG Qiu-Lai, YAN Chao, LIU Shuang, ZHANG Yu-Hui, TIAN Jing-Fen, DENG Yu-Xuan, MA Chun-Mei. Nitrogen accumulation and nitrogen substitution effect of maize under straw returning with continuous cropping [J]. Acta Agronomica Sinica, 2022, 48(4): 962-974.
[11] XU Ning-Kun, LI Bing, CHEN Xiao-Yan, WEI Ya-Kang, LIU Zi-Long, XUE Yong-Kang, CHEN Hong-Yu, WANG Gui-Feng. Genetic analysis and molecular characterization of a novel maize Bt2 gene mutant [J]. Acta Agronomica Sinica, 2022, 48(3): 572-579.
[12] SONG Shi-Qin, YANG Qing-Long, WANG Dan, LYU Yan-Jie, XU Wen-Hua, WEI Wen-Wen, LIU Xiao-Dan, YAO Fan-Yun, CAO Yu-Jun, WANG Yong-Jun, WANG Li-Chun. Relationship between seed morphology, storage substance and chilling tolerance during germination of dominant maize hybrids in Northeast China [J]. Acta Agronomica Sinica, 2022, 48(3): 726-738.
[13] QU Jian-Zhou, FENG Wen-Hao, ZHANG Xing-Hua, XU Shu-Tu, XUE Ji-Quan. Dissecting the genetic architecture of maize kernel size based on genome-wide association study [J]. Acta Agronomica Sinica, 2022, 48(2): 304-319.
[14] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[15] ZHANG Qian, HAN Ben-Gao, ZHANG Bo, SHENG Kai, LI Lan-Tao, WANG Yi-Lun. Reduced application and different combined applications of loss-control urea on summer maize yield and fertilizer efficiency improvement [J]. Acta Agronomica Sinica, 2022, 48(1): 180-192.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd