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Acta Agron Sin ›› 2007, Vol. 33 ›› Issue (02): 317-321.

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DNA Sampling Strategy and Calculation of Genetic Distance between Maize (Zea mays L.) Populations

HOU Ben-Jun1,WANG Tie-Gu12,CHEN Yan-Hui1*,WU Lian-Cheng1,KU Li-Xia1   

  1. 1 Agronomy College, Henan Agricultural University, Zhengzhou 450002, Henan; 2Ion Beam Biotechnology Key Laboratory, Zhengzhou University, Zhengzhou 450052, Henan, China
  • Received:2006-01-04 Revised:1900-01-01 Online:2007-02-12 Published:2007-02-12
  • Contact: CHEN Yan-Hui

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Abstract:

SSR is a good molecule marker with broad application. Bulk sampling is feasible to analyze genetic diversity of maize population by SSR. It is necessary to study influence of genetic distance as using bulk sampling. In present study, the genetic distances between two populations were compared and analyzed by 50 pairs of SSR primers, checked by inbred lines Huangzao 4 and Mo17, and using 60 individuals DNA samples of Golden Queen and Yuzong 5 maize populations with six treatments (the individual DNA samples, the mixed DNA samples with 5 individuals, 10 individuals, 15 individuals, 20 individuals, and 30 individuals, respectively). The results indicated that using the individual DNA samples can detect the alleles with very low frequencies, and make the detected allele number decreased gradually and the frequency of missing alleles increased with the increase of mixed individuals in the treatmen. The results of the genetic distance between two populations calculated by three models suggested that the genetic distances GD, GD(J) and GD(N) showed a gradually increasing trend with the increasing of the mixed individual number, but the increasing extent of GD(J) and GD(N) was wider. There was an obvious deviation between GD(J), GD(N) from mixed samples and the GD from 60 individual DNA samples based on allele frequency. The results and the comparison of the genetic distances based on different calculation methods suggested that the 10 individuals’ DNA mixture is the best sampling strategy when genetic diversity and polymorphism in maize populations are studied by SSR.
If the Nei’s genetic distance GD(N) and Jaccord’s genetic distance GD(J) are calculated according to 0, 1 data, (GD(N)+GD(J))/2 will be calculated close to the genetic distance by allele frequency of individuals. Gene frequency can be studied by using capillary electrophoresis and SSR fluorescence detection. There will be a broad application prospect about bulk sampling strategy with SSR marker.

Key words: SSR, Maize, Genetic distance, Genetic diversity, Sampling method

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