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Acta Agron Sin ›› 2004, Vol. 30 ›› Issue (03): 189-195.

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Isolation of Resistance Gene Candidates by a Resistance Gene Analog of Thinopyrum intermedium and Pooled-PCR

ZHANG Zeng-Yan;XU Jing-Sheng;LIU Yao-Guang;WANG Xiao-Ping;LIN Zhi-Shan;XIN Zhi-Yong   

  1. Key Lab of Crop Genetics and Breeding，Ministry of Agriculture，Institute of Crop Breeding and Cultivation，CAAS，Beijing 100081
  • Received:2003-02-26 Revised:2003-06-26 Online:2004-03-12 Published:2004-03-12
  • Contact: ZHANG Zeng-Yan

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Abstract: Based on the known R gene conserved domains, 10 pairs of degenerate primers were synthesized and used to amplify genomic DNAs of Th. intermedium and translocation line HW642. The PCR products of Th. intermedium were excised and cloned. Clones were sequenced and compared to sequence homolog with gene

Key words: Thinopyrum intermedium, resistance to barley yellow dwarf virus, resistance gene analog（RGA）, transformation-competent artificial chromosome（TAC）, pooled-PCR

CLC Number: 

  • Q78
[1] LI Hong-Jie,WANG Xiao-Ming,CHEN Huai-Gu,LI Wei,LIU Dong-Tao,ZHANG Hui-Yun. Reaction of Wheat-Thinopyrum Progenies and Wheat Germplasm to Sharp Eyespot [J]. Acta Agron Sin, 2013, 39(06): 999-1012.
[2] ZHAO Hai-Bin,ZHANG Yan-Ming,SHI Chun-Long,YAN Xiao-Dan,TIAN Chao,LI Yong-Peng,LI Ji-Lin. Development and Cytogenetic Analysis of Perennial Wheat in Cold Region [J]. Acta Agron Sin, 2012, 38(08): 1378-1386.
[3] ZHAO Dan, ZHAO Ji-Rong, HUANG Qian, LI Ning, HUANG Tie-Jing, ZHANG Ceng-Yan. Functional Analysis of TNBL1 Gene in Wheat Defense Response to Barley yellow dwarf virus Using BSMV-VIGS Technique [J]. Acta Agron Sin, 2011, 37(11): 2106-2110.
[4] LI Zhao, PENG Hong-Chao, DU Li-Pu, ZHOU Miao-Beng, CA Shi-Bin, XU Hui-Jun, LI Shi-Shen, ZHANG Ceng-Yan. Utilization of Tissue Specific Expressing Promoter RSS1P in TiERF1 Transgenic Wheat [J]. Acta Agron Sin, 2011, 37(10): 1897-1903.
[5] LI Ning, HUANG Qian, LIU Yan, ZHAO Dan, LIU Yan, HUANG Tie-Jing, ZHANG Ceng-Yan. Identification and Functional Analysis of a Wheat Resistance Analogous Gene BRG1 [J]. Acta Agron Sin, 2011, 37(06): 998-1004.
[6] WANG Kai;DU Li-Pu;ZHANG Zeng-Yan;LIAO Yong;XU Hui-Jun;YAO Wu-Lan;HUANG Huang;YANG Kun;XIN Zhi-Yong. Isolation and Preliminarily Functional Analysis of SGT1 Gene of Thinopyrum intermedium [J]. Acta Agron Sin, 2008, 34(03): 520-525.
[7] YAO Wu-Lan;ZHANG Zeng-Yan;CHEN Liang. Cloning and Expression of a Pathogen-induced ERF Gene in Thinopyrum intermedium [J]. Acta Agron Sin, 2007, 33(09): 1405-1410.
[8] CUI Zhi-Fu;LIN Zhi-Shan;XIN Zhi-Yong;TANG Yi-Miao;ZHANG Zeng-Yan;LU Qin. Identification of Wheat-Thinopyrum intermedium Telosomic Lines Resistant to Barley Yellow Dwarf Virus by GISH and STS Markers Converted from RFLP [J]. Acta Agron Sin, 2006, 32(12): 1855-1859.
[9] MA You-Zhi;TOMITA Motonori;CAO Li-Xia;LI Lian-Cheng;YASUMURO Yoshimasa. Isolation and Characterisation of a Retrotransposon-Like Sequence Derived from Thinopyrum intermedium [J]. Acta Agron Sin, 2004, 30(04): 209-303.
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