小麦抗病基因类似序列BRG1的分离与功能分析

doi:10.3724/SP.J.1006.2011.00998

Abstract

Abstract: Barley yellow dwarf virus (BYDV) can cause wheat yellow dwarf. In this study, we isolated a fragment of a wheat resistance analogous gene, tentatively named BYDV response gene1 (BRG1),which was expressed in the BYDV resistant wheat-Thinopyrum intermedium translocation line YW642 but was not expressed in the BYDV susceptible wheat Zhong 8601 by using cDNA-AFLP technique.The full-length cDNA sequence of the gene BRG1 was obtained by rapid amplification of cDNA end (RACE) and RT-PCR methods. The gene BRG1 encodes a NBS-LRR protein consisting of 645 amino acid residues，which possesses one typical NB-ARC domain and three leucine-rich domains. The result of expression analysis by Q-RT-PCR method indicated that the expression of BRG1 gene was predominant in the BYDV resistant translocation line YW642 and induced by BYDV infection, reached the peak at 48 hour post inoculation with BYDV, whereas the express level of the gene in the susceptible wheat parent Zhong8601 was lower than that in the resistant wheat YW642, and showed a decline tendency with BYDV infection time. The mRNA expressionof BRG1 gene in YW642 wasup-regulated by salicylic acid (SA) and jasmonate (JA). Virus induced gene silencing technique was used to conduct functional analysis on the gene BRG1. The results showed that after BYDV infection, BYDV relative content in BRG1 knocked-down YW642 was higher than that in YW642 expressing BRG1 gene, whereas the silenced BRG1 gene did not obviously alter the plant phenotype to BYDV infection, suggesting that the gene BRG1 may be involved in the host response to BYDV infection but not be an important gene.

Key words: Wheat-Thinopyrum intermedium translocation line, Resistance to barley yellow dwarf virus (BYDV), cDNA-AFLP, Virus-induced gene silencing

LI Ning, HUANG Qian, LIU Yan, ZHAO Dan, LIU Yan, HUANG Tie-Jing, ZHANG Ceng-Yan. Identification and Functional Analysis of a Wheat Resistance Analogous Gene BRG1[J].Acta Agron Sin, 2011, 37(06): 998-1004.

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Identification and Functional Analysis of a Wheat Resistance Analogous Gene BRG1

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