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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (06): 905-910.doi: 10.3724/SP.J.1006.2010.00905

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Analysis of MADS-box Gene from Soybean (Glycine max L. Merr.) Cytoplasmic Male Sterile Line

 HAN Li-Chao, JIANG Wei, YANG Shou-Ping, YU De-Ti, GAI Jun-Yi   

  1. National Center for Soybean Improvement,National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2009-12-21 Revised:2010-03-19 Online:2010-06-12 Published:2010-04-14
  • Contact: YANG Shuo-Ping,E-mail:spyang@njau.edu.cn,Tel:025-84396463;GAI Jun-Yi,E-mail:sri@njau.edu.cn, Tel: 025-84395405
  • About author:hanlitao0210@yahoo.com.cn

Abstract:

CMS (cytoplasmic male sterility) plays an important role in the utilization of crop heterosis. It is of important significance on theory and practice to study the genetic base and mechanism of CMS. To reveal the molecular mechanism of soybean cytoplasmic male sterility, we analyzed the gene differential expression between the soybean cytoplasmic male sterile line NJCMS2A and its maintainer line NJCMS2B by the cDNA-AFLP differential display method. A differentially expressed fragment from the flower buds of NJCMS2A was cloned and sequenced. The Blast results showed that the differentially expressed fragment shared 98.7% homology with g29510.1 cDNA fragment on Gm13 of soybean genome, and 98% homology with a MADS-boxgene of soybean. The Blast results of the amino acid sequences indicated that the differentially expressed fragment shared 96% homology with a MADS-box protein of Glycine max, 83% homology with the MADS-box M7 protein of Pisum sativum, 88% homology with the MADS-box 2 protein of Momordica charantia, and 83% homology with AGAMOUS protein of Gossypium barbadense. The structure and function prediction of the amino acid sequence showed that the protein encoded by the differentially expressed fragment was the transcription factor of the MADS-box gene which contained a typical K-box domain. The results of semi-quantitative RT-PCR showed that the expression quantity of the differentially expressed fragment in the flower buds of NJCMS2A was much higher than that of NJCMS2B. According to the above results, it was inferred that the differentially expressed fragment was probably related to the soybean cytoplasmic male sterility.

Key words: Soybean, Cytoplasmic male sterility, cDNA-AFLP, MADS-box transcription factor

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