硅及其吸收基因Lsi1调节水稻耐UV-B辐射的作用

doi:10.3724/SP.J.1006.2011.01005

Abstract

Abstract: Silicon (Si) has beneficial function in enhancing plant resistance to biotic and abiotic stresses. Rice is a typical Si-accumulating plant. In this research, UV-B tolerant rice accession Lemont, UV-B sensitivity rice Dualr and their low silicon rice gene 1 (Lsi1) transgenic lines were used to explore the relationships between silicon and rice UV-B tolerance. It was found that rice cultured in Si-deficiency solution had lower gene transcript levels of phenylalanine ammonia lyase (PAL), photolyase (PL), and lower contents of total phenolics and flavonoids in leaves than that in Si-containing solution. The same tendency was found in the case when the rice accessions were supplementarily exposed to UV-B radiation, although both gene expression level and antioxidants concentrations were increased. Further, Lsi1-suppressed or overexpressedtransgenic rice lines of Lemont, and Lsi1-overexpressed transgenic rice line of Dular were also detected in the same treatments. The results showed that gene transcript level of PAL and PL was increased in Lsi1-overexpressed transgenic line, but down-regulated in Lsi1-RNAi line of Lemont as compared with their wild types (WT) under normal light condition. The expression level of the two genes in all entries was enhanced after UV-B radiation treatment, and it was the highestin Lsi1-overexpressed line of Lemont, followed by their WT, and lowest in Lsi1-RNAi line. The same tendency was also found in the content of total phenolics and flavonoids. The similar results were further confirmed in the overexpression of Lsi1 in Dular.The findings suggested that rice UV-B tolerance could be effectively mediated by enhancing/inhibiting expression of Lsi1.

Key words: Silicon, Lsi1, Rice (Oryza sativa L.), Ultraviolet-B

FANG Chang-Jun, WANG Qing-Shui, YU Pan, HUANG Li-Kun, WU Xing-Chun, LIN Wen-Xiong. Silicon and Its Uptaking Gene Lsi1 in Regulation of Rice UV-B Tolerance[J].Acta Agron Sin, 2011, 37(06): 1005-1011.

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Silicon and Its Uptaking Gene Lsi1 in Regulation of Rice UV-B Tolerance

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