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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (12): 2123-2134.doi: 10.3724/SP.J.1006.2013.02123

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Gene Differential Expression of a Green-revertible Albino and High-tillering Dwarf Mutant hfa-1 by Using Rice Microarray

GUO Tao**,HUANG Yong-Xiang**,LUO Wen-Long,HUANG Xuan,WANG Hui,CHEN Zhi-Qiang*,LIU Yong-Zhu*   

  1. South China Agricultural University, National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China?
  • Received:2013-03-26 Revised:2013-06-20 Online:2013-12-12 Published:2013-09-29
  • Contact: 刘永柱, E-mail: lively@scau.edu.cn; 陈志强, E-mail: chenlin@scau.edu.cn

Abstract:

Phenotype of the hfa-1 mutant characterized by green-revertible albino and high-tillering dwarf is controlled by a single recessive nuclear gene hw-1(t), which encodes a chloroplast protein containing the mitochondrial alternative oxidase (AOX) structure and involves in the electron transport chain of chloroplast respiratory regulating biosynthesis of carotenoid and carotenoid-related secondary metabolic pathways. In this study, expression analysis of genes involved in carotenoid biosynthesis and carotenoid-related plant hormones biosynthesis andgene-expression profiling were conducted before and after the turning-green of hfa-1 mutant. the results displayed that expression levels of genes involved in carotenoid biosynthesis and carotenoid-related plant hormones (GA, ABA and SL) biosynthesis were decreased in the albino leaves of hfa-1, suggesting that mutation of hw-1(t) inhibits the biosynthesis of carotenoid and has an impact on carotenoid-related secondary metabolic pathways. Gene expression profiling and functional classification showed that genes related to physiological processes in response to photoeynthesis, endogenous stimulus and stress were differently expressed in hfa-1 before and after its turning-green. Expression of genes encoding proteins related to the electron transport complex Cytb6/f were up-regulated significantly, indicating that Cytb6/f would provide some compensation in electron transport and redox of plastoquinone in the hfa-1 mutant.

Key words: Rice (Oryza sativa L.), Green-revertible albino, hfa-1 mutant, Gene expression analysis

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