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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (11): 1596-1602.doi: 10.3724/SP.J.1006.2017.01596


Identification of QTLs Conferring Small Brown Planthopper Resistance in Rice (Oryza sativa L.) Using MR1523/Suyunuo F2:3 Population

ZHONG Jie1,WEN Pei-Zheng1,SUN Zhi-Guang1,XIAO Shi-Zhuo1,HU Jin-Long1,ZHANG Le1,JIANG Ling1,CHENG Xia-Nian1,LIU Yu-Qiang1,*,WAN Jian-Min1,2   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2017-04-18 Revised:2017-07-23 Online:2017-11-12 Published:2017-08-11
  • Contact: 刘裕强, E-mail: yql@njau.edu.cn E-mail:2014101003@njau.edu.cn
  • Supported by:

    This study was supported by the National Key R&D Program of China (2017YFD0100400-01), National Natural Science Foundation of China (31522039, 31471470), and the Natural Science Foundation of Jiangsu Province of China (BK20150026)


The small brown planthopper (SBPH), Laodelphax striatellus Fallén (Homoptera: Delphacide), is one of the most destructive insect pests in rice (Oryza sativa L.) production. SBPH not only causes direct damage by sucking plant sap but also transmits the main viral diseases, which seriously threatens the safety of rice production. An indica cultivar MR1523 displayed strong antixenosis against SBPH. In order to identify SBPH resistance genes, a F2:3 population derived from a cross between MR1523 and susceptible japonica cultivar Suyunuo was constructed, and then evaluated for SBPH resistance. Moreover, 120 polymorphic SSR markers between the parents uniformly distributed on 12 chromosomes of rice were used to construct the molecular linkage map, and then conducted the QTL assay for SBPH resistance. A total of four QTLs on chromosome 2, 5, and 6, Qsbph2, Qsbph5a, Qsbph5b, and Qsbph6 were detected in the interval of RM526–RM3763, RM17804–RM13, RM574–RM169, and RM190–RM510, with LOD score of 2.14, 3.13, 3.23, and 2.35, and explained the phenotypic variance of 12.0%, 14.7%, 17.4%, and 14.1% , respectively. All of the QTLs came from the resistance parent MR1523. The results in this study lay a foundation for furtherly fine mapping SBPH resistance and developing new cultivars by molecular marker assisted selection in the future.

Key words: Rice (Oryza sativa L.), Small brown planthopper (SBPH);Resistance, Quantitative trait locus (QTL)

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