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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (01): 63-71.doi: 10.3724/SP.J.1006.2017.00063


Fine Mapping andGenetic Effect Analysis of qKRN5.04, a Major QTL Associated with Kernel Row Number

BAI Na,LI Yong-Xiang*,JIAO Fu-Chao,CHEN Lin,LI Chun-Hui,ZHANG Deng-Feng,SONG Yan-Chun,WANG Tian-Yu,LI Yu,SHI Yun-Su*   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China?
  • Received:2016-04-05 Revised:2016-06-20 Online:2017-01-12 Published:2016-07-28
  • Contact: 李永祥, E-mail: liyongxiang@caas.cn; 石云素, E-mail: shiyunsu@caas.cn
  • Supported by:

    This study was supported by the Special Fund for Agro-scientific Research in the Public Interest, the Special Fund for Protection and Utilization of Crop Germplasm Resources (201303007, 2015NWB030-04), the National Key Technology Support Program of China(2013BAD01B02-3), the National Natural Science Foundation of China(91335206), and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.


For kernel row number (KRN), one of the important factors of maize yield, the genetic basis dissection and fine mapping of crucial gene would be greatly beneficial to maize molecular breeding. In present study, series of advanced backcross population and secondary segregation population were developed from a backcross of the derived line of four-row waxy corn (four kernel rows, as the donor parent) and Nong531 (18-22 kernel rows, as the recurrent parent). The QTL mapping for KRN was conducted by the inclusive complete interval mapping (ICIM) method in multiple environments. And a major KRN QTL, qKRN5.04, was mapped to the interval of 136.3-140.0 Mb on chromosome 5, with the largest phenotypic variation of 21.76% and the effect value of 0.80-1.76 row. Furthermore, according to recombinant analysis of secondary population, qKRN5.04 was fine mapped to the region of about 300 kb, which provided both practical InDel markers for marker-assisted selection and sufficient supports for the map-based cloning and candidate gene mining of the target locus.

Key words: Maize, Kernel row number (KRN), Quantitative trait locus (QTL), Fine mapping

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