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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2090-2097.doi: 10.3724/SP.J.1006.2014.02090


Functional Characterization of the Glycoside Hydrolase Encoding Gene OsBE1 during Chloroplast Development in Oryza sativa

WANG Xing-Chun1,2,*,WANG Min1,#,JI Zhi-Juan3,CHEN Zhao4,LIU Wen-Zhen3,HAN Yuan-Huai2,YANG Chang-Deng3,*   

  1. 1 College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China; 2 Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu 030801, China; 3 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 4 College of Arts and Sciences, Shanxi Agricultural University, Taigu 030801, China
  • Received:2014-07-16 Revised:2014-09-16 Online:2014-12-12 Published:2014-10-20
  • Contact: 王兴春, E-mail: wxingchun@163.com, Tel: 0354-6287191-307; 杨长登, E-mail: yangchangdeng@yahoo.com.cn, Tel: 0571-63370367


Chloroplast plays an important role in plant carbohydrate metabolism; however the function of carbohydrate metabolism in the chloroplast development is poorly understood. The Oryza sativa Branching Enzyme 1 (OsBE1) gene, encoding a glycoside hydrolase family 13 protein, was cloned from rice. The identity between OsBE1 and Arabidopsis AtBE1 is 66%, but only 40% between OsBE1 and the classical starch branching enzymes in rice. A T-DNA insertion mutant of OsBE1 gene was identified. The seedlings of the osbe1 mutant were albino, and died at the three-leaf stage. This albino phenotype could not be rescued by exogenous carbohydrate. Starch staining showed no obvious difference in starch content between osbe1 and the wild type. Further studies showed that there were less chloroplasts in osbe1 mutant than in wild type, and there was no obvious stroma lamella in the osbe1 chloroplast. The overexpression vector pCAMBIA1300-35S-OsBE1 was constructed and transformed into rice variety Zhonghua 11. Finally, 108 transgenic lines were obtained and 77 lines of them showed etiolation in different degrees. The work not only sheds a novel insight into the regulation mechanism of carbohydrate metabolism on chloroplast development, but also lays a foundation for further understanding of the OsBE1’s function.

Key words: Glycoside hydrolase, Carbohydrate metabolism, OsBE1, Chloroplast, Rice (Oryza sativa L.)

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