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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (05): 650-656.doi: 10.3724/SP.J.1006.2018.00650

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Expression Pattern and Protein Localization of a Yellow-Green Leaf 6 (YGL6) Gene in Rice (Oryza sativa)

Jun-Qiong SHI1,2(), Ya-Qin WANG1, Tian-Quan ZHANG1, Ling MA1, Guang-Hua HE1,*()   

  1. 1 Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China
    2 School of Life Sciences, Southwest University, Chongqing 400716, China
  • Received:2017-09-23 Accepted:2018-01-08 Online:2018-05-20 Published:2018-01-29
  • Contact: Guang-Hua HE E-mail:shijunqiong@163.com;hegh@swu.edu.cn
  • Supported by:
    This study was supported by the National Key Research and Development Program of China (2017YFD0100201), Chongqing Science and Technology Commission Project (CSTCCXLJRC201713, cstc2016shms-ztzx0017), and the Fundamental Research Funds for the Central Universities (XDJK2016C111).

Abstract:

Leaf color mutants are used not only as morphological markers in hybrid rice breeding, but also as ideal materials in studies on the structure and function of photosystem, chlorophyll biosynthesis and regulation mechanism. A new rice mutant exhibiting stable inheritance was derived from ethyl methane sulfonate (EMS)-treated restorer line Jinhui 10 (Oryza sativa), tentatively named as yellow-green leaf 6 (ygl6). The ygl6 leaf displayed yellow-green at seeding stage, and pale green at jointing stages. The YGL6 complementation experiment implied that the Os12g23180 is the YGL6 gene. The expression pattern analysis indicated that YGL6 was expressed in green tissues including young leaves, mature leaves, sheaths and green glume, with the highest expression level in young leaves. And YGL6 expression was induced by light. Transient expression of the YGL6-GFP protein in rice protoplast showed that YGL6 was localized in chloroplasts. These results provide a foundation for functional analysis of YGL6.

Key words: rice (Oryza sativa), yellow-green leaf 6 (ygl6), YGL6 gene, chloroplast, expression pattern

Fig. 1

Functional complementation analysis of YGL6 A: phenotypes of WT, ygl6 mutant, and transgenic plant (ygl6/YGL6); B: leaves of WT, ygl6 mutant, and transgenic plant; C: the YGL6 sequencing of WT, ygl6 mutant, and transgenic plant; D: pigment contents of WT, ygl6 mutant, and transgenic plant. *P﹤0.05."

Fig. 2

Phylogenetic analysis of the YGL6"

Fig. 3

Expression analysis of YGL6 by qRT-PCR A: expression level of YGL6 at seedling stage in WT and the YGL6 mutant; B: expression level of YGL6 at booting stage in WT. *P﹤0.05."

Fig. 4

qRT-PCR analysis of the expression level of YGL6 A: expression level of YGL6 in wild-type plants at different hours transferred to continuous light from continuous darkness; B: expression level of YGL6 in wild-type plants at different hours transferred to continuous darkness from continuous light."

Fig. 5

Subcellular location of YGL6 protein using rice protoplasts A-D: negative control (35S-GFP); E-H: subcellular location of YGL6 protein (35S-YGL6ORF-GFP)."

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