花生膜联蛋白基因家族成员的结构和表达分析

doi:10.3724/SP.J.1006.2019.84056

Abstract

Abstract:

Annexin is a kind of calcium-dependent phospholipid binding proteins involved in the regulation of plant metabolism, growth and development, drought resistance and salt tolerance, and its structure is species-specific in different plants. In order to have a systematic analysis of the annexin gene family of peanut, we identified 30 annexin genes from the peanut genome database, and analyzed their characteristics using bioinformatics method. Peanut annexin (annexin of Arachis hypogaea, AnnAh) genes were unevenly distributed on 13 chromosomes, with 13 in A genome and 17 in B genome. There were 2 to 8 introns in the AnnAhs, with 5 to 6 introns in most AnnAhs. Phylogenetic analysis showed that the clustering relationship was complex. The annexins of lower plant, monocotyledonous and dicotyledonous plants were distributed at interval, with AnnAhs inserting in each branch. However, in each small branch, AnnAhs were basically clustered with the dicotyledonous plant annexins, and close to soybean, alfalfa and sunflower, followed by Arabidopsis; but several AnnAhs were associated with monocotyledonous and lower plant annexins. All 30 AnnAhs had no transmembrane domain, and 16 of them were located in cytoplasm, and the others’ localization was uncertain. Results of alternative splicing (AS) analysis of AnnAhs showed that only 11 AnnAhs experienced AS, which accounted for about 38% of all AnnAhs; the AS events occurred most in roots, followed by leaves, and the least in seeds. The expression level of AnnAhs was high in seed2 and root, followed by seed1, and lower in leaf. The comprehensive analysis of AnnAhs can provide some theoretical support for peanut resistance breeding.

Key words: peanut, annexin, gene structure analysis, alternative splicing analysis, expression pattern analysis

Hui-Min WANG,Xin-Guo LI,Shu-Bo WAN,Zhi-Meng ZHANG,Hong DING,Guo-Wei LI,Wen-Wei GAO,Zhen-Ying PENG. Structure and expression analysis of the members of peanut annexin gene family[J].Acta Agronomica Sinica, 2019, 45(3): 390-400.

Figures/Tables 6

Table 1

Anneixn genes family in peanut"

基因名称 Gene name	染色体位置 Chr. position	氨基酸个数 Amino acid number	亚细胞定位 Subcellular localization	蛋白完整性 Protein integrity	Annexin 结构域 Annexin domain	重复序列 Repetitive sequence	其他结构域 Other domains
Aradu.V26BD	Aradu.A03	279	C	完整 Complete	4	4	0
Aradu.86DER	Aradu.A03	286	–	不完整 Incomplete	4	3	0
Aradu.KJ1YM	Aradu.A04	274	–	不完整 Incomplete	3	2	0
Aradu.3L5NK	Aradu.A05	315	C	完整 Complete	4	4	0
Aradu.WYZ5E	Aradu.A05	323	C	不完整 Incomplete	4	4	0
Aradu.9BC7H	Aradu.A07	342	C	不完整 Incomplete	4	4	0
Aradu.23XWK	Aradu.A08	309	C	不完整 Incomplete	3	3	0
Aradu.N8MUP	Aradu.A08	279	–	不完整 Incomplete	3	3	0
Aradu.4J11T	Aradu.A08	339	–	不完整 Incomplete	3	3	0
Aradu.S16C5	Aradu.A08	321	–	完整 Complete	4	4	0
Aradu.SCC75	Aradu.A10	266	C	不完整 Incomplete	3	3	0
Aradu.IZQ3Z	Aradu.A10	315	C	完整 Complete	4	4	0
Aradu.MBZ2M	Aradu.A10	333	–	完整 Complete	2	2	0
Araip.1LR8I	Araip.B02	89	C	不完整 Incomplete	2	2	0
Araip.KR6F4	Araip.B03	323	–	完整 Complete	2	2	2
Araip.X0F2S	Araip.B03	317	–	完整 Complete	4	4	0
Araip.CCM9G	Araip.B03	286	–	不完整 Incomplete	4	3	0
Araip.RGY04	Araip.B04	142	C	不完整 Incomplete	1	1	0
Araip.R8WRM	Araip.B04	207	–	不完整 Incomplete	3	2	0
Araip.HD6QL	Araip.B05	316	C	完整 Complete	4	4	0
Araip.0MR1X	Araip.B05	315	C	完整 Complete	4	4	0
Araip.FX5SI	Araip.B07	316	C	完整 Complete	4	4	0
Araip.J58EQ	Araip.B08	135	C	不完整 Incomplete	2	2	0
Araip.YGP4J	Araip.B08	315	–	完整 Complete	3	3	0
Araip.6KP6U	Araip.B08	295	–	完整 Complete	3	3	0
Araip.Y8EDR	Araip.B08	321	–	完整 Complete	4	4	0
Araip.X9BIG	Araip.B10	317	C	完整 Complete	4	4	0
Araip.Z0Q6Q	Araip.B10	311	C	完整 Complete	4	3	0
Araip.WA456	Araip.B10	315	C	完整 Complete	4	3	0
Araip.RPP1M	Araip.B10	364	–	完整 Complete	2	2	0
Aradu.V26BD	Aradu.A03	279	C	完整 Complete	4	4	0
Aradu.86DER	Aradu.A03	286	–	不完整 Incomplete	4	3	0

Table 1

Fig. 1

Fig. 2

Fig. 3

Table 2

Fig. 4

References 43

[1]	Ahmed I, Yadav D, Shukla P, Kirti P B . Heterologous expression of Brassica juncea annexin, AnnBj2 confers salt tolerance and ABA insensitivity in transgenic tobacco seedlings. Funct Integr Genomic, 2018,18:569-529. doi: 10.1007/s10142-018-0614-z
[2]	李瑞梅, 王雨晴, 张帆, 袁帅, 耿梦婷, 姚远, 刘姣, 段瑞军, 符少萍, 胡新文, 郭建春 . 植物膜联蛋白(Annexin)的研究进展. 基因组学与应用生物学, 2017,36:2128-2136.
	Li R M, Wang Y Q, Zhang F, Yuan S, Geng M T, Yao Y, Liu J, Duan R G, Fu S P, Hu X W, Guo J C . Research progress on plant annexin. Genomic Appl Biol, 2017,36:2128-2136 (in Chinese with English abstract).
[3]	叶卫华 . 膜联蛋白A7(annexin A7)在胃癌凋亡中作用的研究 . 河北医科大学博士学位论文, 河北石家庄, 2017.
	Ye W H . The Role of A7(Annexin A7) in Apoptosis of Gastric Carcinoma. PhD Dissertation of Hebei Medical University, Shijiazhuang, Hebei, China, 2017 (in Chinese with English abstract).
[4]	Galvão I, Vago J P, Barroso L C, Tavares L P ,Queiroz-Junior C M, Costa V V, Carneiro F S, Ferreira T P, Silva P M, Amaral F A, Sousa L P, Teixeira M M. Annexin A1 promotes timely resolution of inflammation in murine gout. Eur J Immunol, 2017,47:585-596. doi: 10.1002/eji.201646551 pmid: 27995621
[5]	Konopkapostupolska D, Clark G . Annexins as overlooked regulators of membrane trafficking in plant cells. Int J Mol Sci, 2017,18:863. doi: 10.3390/ijms18040863 pmid: 5412444
[6]	Hua K L, Li Y, Zhao Q, Fan L Q, Tan B B, Gu J B . Downregulation of Annexin A11 (ANXA11) inhibits cell proliferation, invasion, and migration via the AKT/GSK-3β pathway in gastric cancer. Med Sci Monitor, 2018,24:149-160. doi: 10.12659/MSM.905372 pmid: 29306955
[7]	Pi F, Zhang H, Li H, Thiviyanathan V, Gorenstein D G, Sood A K, Guo P . RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery. Nanomedicine(UK), 2017,13:1183-1193. doi: 10.1016/j.nano.2016.11.015 pmid: 27890659
[8]	Yu S, Bian H, Gao X, Gui L . Annexin A9 promotes invasion and metastasis of colorectal cancer and predicts poor prognosis. Int J Mol Med, 2018,41:2185-2192. pmid: 29393380
[9]	杜锐 . Annexin A3 (ANXA3)对乳腺癌的调控研究与乳腺癌患者来源移植瘤模型的建立. 中国科学技术大学博士学位论文, 安徽合肥, 2017.
	Du R . The Role of Annexin A3 in Regulating Breast Cancer and the Establishment of Breast Cancer PDX Model. PhD Dissertation of University of Science and Technology of China, Hefei, Anhui, China, 2017 (in Chinese with English abstract).
[10]	刘琰 . Annexin A2对卵巢癌生物学行为的影响及机制研究 . 郑州大学博士学位论文, 河南郑州, 2017.
	Liu Y . The Effects and Mechanism of Annexin A2 on Biological Behavior of Ovarian Cancer. PhD Dissertation of Zhengzhou University, Zhengzhou, Henan, China, 2017 (in Chinese with English abstract).
[11]	Xu L, Tang Y M, Gao S Q, Su S C, Hong L, Wang W W, Fang Z F, Li X Y, Ma J X, Quan W, Sun H, Li X, Wang Y B, Liao X Z, Gao J G, Zhang F G, Li L, Zhao C P . Comprehensive analyses of the annexin gene family in wheat. BMC Genomics, 2016,17:415. doi: 10.1186/s12864-016-2750-y pmid: 4884362
[12]	Zhang F, Jin X X, Wang L K, Li S F, Wu S, Cheng C Z, Zhang T Z, Guo W Z . A cotton annexin affects fiber elongation and secondary cell wall biosynthesis associated with Ca ²+ influx, ROS homeostasis, and actin filament reorganization . Plant Physiol, 2016,171:1750-1770. doi: 10.1104/pp.16.00597
[13]	楚璞 . 莲膜联蛋白的鉴定及其在种子耐热性和活力中的功能研究. 中山大学博士学位论文, 广东广州, 2011.
	Chu P . Identification and Functional Analysis of a Nelumbo nuciferaa Annexin Involved in Seed Thermotolerance and Vigor. PhD Dissertation of Sun Yat-Sen University. Guangzhou, Guangdong, China, 2011 (in Chinese with English abstract).
[14]	褚翠萍 . 拟南芥膜联蛋白的基因表达、亚细胞定位和AnnAt3响应非生物胁迫的初步研究. 山东农业大学硕士学位论文, 山东泰安, 2012. doi: 10.7666/d.y2116641
	Chu C P . Gene Expression, Subcellular Localization of Arabidopsis Annexins and Abiotic Stress Response of Ann At3. MS Thesis of Shandong Agricultural University, Tai’an, Shandong, China, 2005 (in Chinese with English abstract). doi: 10.7666/d.y2116641
[15]	何美敬, 穆国俊, 侯名语, 陈焕英, 崔顺立, 杨鑫雷, 刘立峰 . 植物膜联蛋白的结构及功能研究进展. 西北植物学报, 2013,33:2567-2574.
	He M J, Mu G J, Hou M Y, Chen H Y, Cui S L, Yang X L, Liu L F . Advances in structure and function of plant annexins. Acta Bot Boreali-Occid Sin, 2013,33:2567-2574 (in Chinese with English abstract).
[16]	褚翠萍, 任秋萍, 孔兰静, 王秀玲 . 拟南芥膜联蛋白2的亚细胞定位研究. 中国细胞生物学学报, 2017,39:873-880.
	Zhu C P, Ren Q P, Kong L G, Wang X L . The subcellular localization of annexin 2 in Arabidopsis. Chin J Cell Biol, 2017,39:873-880 (in Chinese with English abstract).
[17]	Clark G B, Dauwalder M, Roux S J . Purification and immunolocalization of an annexin-like protein in pea seedlings. Planta, 1992,187:1-9. doi: 10.1007/BF00201617 pmid: 11538119
[18]	Clark G B, Dauwalder M, Roux S J . Immunological and biochemical evidence for nuclear localization of annexin in peas. Plant Physiol Biochem, 1998,36:621-627. doi: 10.1016/S0981-9428(98)80010-7 pmid: 11542469
[19]	Seals D F, Parrish M L, Randall S K . A 42-kilodalton annexin-like protein is associated with plant vacuoles. Plant Physiol, 1994,106:1403-1412. doi: 10.1104/pp.106.4.1403
[20]	Thonat C, Mathieu C, Crevecoeur M, Penel C, Gaspar T, Boyer N . Effects of a mechanical stimulation of localization of annexin-like proteins in Bryonia dioica internodes. Plant Physiol, 1997,114:981-988. doi: 10.1093/molehr/gam026 pmid: 9232879
[21]	郑昌林 . 花生基因组中重复基因间基因置换的比较分析. 华北理工大学硕士学位论文, 河北唐山, 2017.
	Zheng C L . Comparative Analysis of Repetitive Gene Replacement in Peanut Genome. MS Thesis of North China University of Science and Technology, Tangshan, Hebei, China, 2017 (in Chinese with English abstract).
[22]	王靖 . 花生膜联蛋白基因AnnAh1和AnnAh2的功能验证 . 山东大学硕士学位论文,山东济南, 2014.
	Wang J . Functional Research of Annexin Gene AnnAh1 and AnnAh2 from Arachis hypogaea L. MS Thesis of Shandong University, Jinan, Shandong, China, 2017 (in Chinese with English abstract).
[23]	何美敬, 杨鑫雷, 崔顺立, 穆国俊, 侯名语, 陈焕英, 刘立峰 . 花生膜联蛋白基因(Annexin)家族的分子克隆和鉴定. 中国作物学会学术年会论文集, 2014.
	He M J, Yang X L, Cui S L, Mu G J, Hou M Y, Chen H Y, Liu L F . Molecular cloning and identification of annexin gene family from peanut. In: Proceedings of the Crop Science Society of China, 2014(abstract in Chinese).
[24]	Trapnell C, Williams B A, Pertea G, Mortazavi A ,Kwan G, van Baren M J, Salzberg S L, Wold B J . Transcript assembly and quantification by RNA Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol, 2010,28:511-515. doi: 10.1038/nbt.1621 pmid: 20436464
[25]	Florea L, Li S, Salzberg S L . Thousands of exon skipping events differentiate among splicing patterns in sixteen human tissues. Eur PMC, 2013,2:188. doi: 10.12688/f1000research.2-188.v1 pmid: 3892928
[26]	Clark G B, Dauwalder M, Roux S J . Immunolocalization of an annexin-like protein in corn. Adv Space Res, 1994,14:341-346. doi: 10.1016/0273-1177(94)90421-9 pmid: 11537937
[27]	Jami S K, Clark G B, Ayele B T, Ashe P, Kirti P B . Genome-wide comparative analysis of annexin superfamily in plants. PLoS One, 2012,7:47801. doi: 10.1371/journal.pone.0047801 pmid: 23133603
[28]	王立科, 张边江, 唐宁, 杨平, 周峰, 陈全战 . 植物膜联蛋白基因研究进展. 分子植物育种, 2016,14:2060-2065.
	Wang L K, Zhang B J, Tang N, Yang P, Zhou F, Chen Q Z . Research advances of annexin genes in plants. Mol Plant Breed, 2016,14:2060-2065 (in Chinese with English abstract).
[29]	Vogel C, Chothia C . Protein family expansions and biological complexity. PLoS Comput Biol, 2006,2:48. doi: 10.1371/journal.pcbi.0020048 pmid: 16733546
[30]	Schmutz J, Cannon S B, Schlueter J, Ma J X, Mitros T, Nelson W, Hyten D L, Song Q J, Thelen J J, Cheng J L, Xu D, Hellsten U, May G D, Yu Y, Sakurai T, Umezawa T, Bhattacharyya M K, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S Q, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J C, Tian Z X, Zhu L C, Gill N, Joshi T, Libault M, Sethuraman A, Zhang X C, Shinozaki K, Nguyen H T, Wing R A, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker R C, Jackson S A . Genome sequence of the palaeopolyploid soybean. Nature, 2010,463:178-183. doi: 10.1038/nature08957
[31]	Kandasamy M K, Burgos-Rivera B ,McKinney E C, Ruzicka D R, Meagher R B . Class-specific interaction of profilin and ADF isovariants with actin in the regulation of plant development. Plant Cell, 2007,19:3111-3126. doi: 10.1105/tpc.107.052621
[32]	Koehler P E, Odell G V . Factors affecting the formation of pyrazine compounds in sugara-mine reactions. J Agric Food Chem, 1970,18:895-898. doi: 10.1021/jf60171a041
[33]	Clark G B, Sessions A, Eastburn D J, Roux S J . Differential expression of members of the annexin multigene family in Arabidopsis. Plant Physiol, 2001,126:1072-1084. doi: 10.1104/pp.126.3.1072
[34]	李立奇, 万瑛 . 蛋白质的亚细胞定位预测研究进展. 免疫学杂志, 2009,25:602-604.
	Li L Q, Wan Y . Advancement of predicting proteins in subcellular localization sites. Immunol J, 2009,25:602-604 (in Chinese with English abstract).
[35]	李兵. 棉花( Gossypium hirsutum)膜联蛋白基因克隆鉴定及功能研究. 华中师范大学博士学位论文, 湖北武汉, 2012.
	Li B . Molecular Characterization and Function of the Cotton Annexin Genes. PhD Dissertation of Central China normal University, Wuhan, Hubei, China, 2012 (in Chinese with English abstract).
[36]	王雨晴, 张帆, 李瑞梅, 胡新文, 郭建春 . 木薯MeAnn1基因的克隆及表达分析. 分子植物育种, 2015,13:2477-2483. doi: 10.13560/j.cnki.biotech.bull.1985.2015.10.021
	Wang Y Q, Zhang F, Li R M, Hu X W, Guo J C . Cloning and expression of MeAnn1 gene from cassava(Manihot esculenta Crantz). Mol plant breed, 2015,13:2477-2483 (in Chinese with English abstract). doi: 10.13560/j.cnki.biotech.bull.1985.2015.10.021
[37]	Breton G, Vazqueztello A, Danyluk J, Sarhan F . Two novel intrinsic annexins accumulate in wheat membranes in response to low temperature. Plant Cell Physiol, 2000,41:177-184. doi: 10.1093/pcp/41.2.177 pmid: 10795312
[38]	Carvalho-Niebel F D, Timmers A C J, Chabaud M, Defaux-Petras A, Barker D G . The Nod factor-elicited annexin MtAnn1 is preferentially localised at the nuclear periphery in symbiotically activated root tissues of Medicago truncatula. Plant J, 2002,32:343-352. doi: 10.1046/j.1365-313X.2002.01429.x pmid: 12410812
[39]	章天骄 . 可变剪接的生物信息数据分析综述. 生物信息学, 2012,10:61-64. doi: 10.3969/j.issn.1672-5565.2012.01.13
	Zhang T J . Review of bioinformation data analysis with alternative splicing. Chin J Bioinformatic, 2012,10:61-64 (in Chinese with English abstract). doi: 10.3969/j.issn.1672-5565.2012.01.13
[40]	Wilkinson J Q, Lanahan M B, Conner T W, Klee H J . Identification of mRNAs with enhanced expression in ripening strawberry fruit using polymerase chain reaction differential display. Plant Mol Biol, 1995,27:1097-1108. doi: 10.1007/BF00020883 pmid: 7766892
[41]	那杰, 杨怀义 . 植物Annexins研究进展. 辽宁师范大学学报(自然科学版), 2004,27:464-467. doi: 10.3969/j.issn.1000-1735.2004.04.023
	Na J, Yang H Y . Aadvances in the study of plant annexins. J Liaoning Norm Univ(Nat Sci Edn), 2004,27:464-467 (in Chinese with English abstract) doi: 10.3969/j.issn.1000-1735.2004.04.023
[42]	Baucher M, Oukouomi L Y, Vandeputte O M, Bopopi J M, Moussawi J, Vermeersch M, Mol A, Jaziri M E, Homble F, Perez-Morga D . Ntann12 annexin expression is induced by auxin in tobacco roots. J Exp Bot, 2011,62:4055-4065. doi: 10.1093/jxb/err112 pmid: 3134359
[43]	巩子英, 宋雪, 陈功友, 朱家璧, 俞冠翘, 邹华松 . 苜蓿膜联蛋白MtAnn3基因的鉴定及其在根毛发育中的功能. 科学通报, 2012,57:431-437. doi: 10.1360/csb2012-57-6-431
	Gong Z Y, Song X, Chen G Y, Zhu J B, Yu G Q, Zou H S . Identification of MtAnn3 gene of alfalfa and its function in root hair development. Chin Sci Bull, 2012,57:431-437 (in Chinese). doi: 10.1360/csb2012-57-6-431

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基因名称 Name	种子前期 Seed1	种子后期 Seed2	根 Root	叶 Leaf
Aradu.S16C5	—	—	TSS	—
Aradu.N8MUP	—	—	TSS	—
Aradu.3L5NK	—	—	—	TTS
Araip.CCM9G	—	TTS, AE	—	TTS, AE
Araip.WA456	—	—	TTS, AE	—
Aradu.IZQ3Z	TTS, AE	AE	AE	AE
Araip.HD6QL	TSS, ES	TSS, ES	—	—
Aradu.KJ1YM	TTS	—	TTS	TTS, AE, ES
Araip.Z0Q6Q	—	—	ES	—
Araip.R8WRM	—	—	ES	—
Araip.RPP1M	—	—	ES	—

Structure and expression analysis of the members of peanut annexin gene family

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