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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (07): 974-982.doi: 10.3724/SP.J.1006.2017.00974


Identification of Rice Chromosome Segment Substitution Line Z519 with Purple Sheath and Candidate Gene Analysis of PSH1

ZHOU Ke1,**,LI Yan1,2,**,WANG Shi-Ming1,CUI Guo-Qing1,YANG Zheng-Lin1,HE Guang-Hua1,LING Ying-Hua1,ZHAO Fang-Ming1,*   

  1. 1 Rice Research Institute, Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Chongqing 400716, China; 2 Tea Research Institute of Guizhou Province, Guiyang 550006, China
  • Received:2016-11-25 Revised:2017-03-01 Online:2017-07-12 Published:2017-03-27
  • Contact: Zhao Fangming, E-mail: zhaofangming2004@163.com, Tel: 023-68250486 E-mail:960968356@qq.com
  • Supported by:

    The research was supported by subject “New Technology of Heterosis Utilization in Rice” (2016YFD0101107) in the National Key Research and Development Program (SQ2016ZY03001818), and Chongqing Key Laboratory Capacity Improvement Project (cstc2014pt-sy80001).


Anthocyanins as plant pigments are widely liked by people and play a very important role in food processing and hybrid purity identification. Here, a rice chromosome segment substitution line (CSSL) Z519 with purple sheath was identified deriving from recipient Nipponbare and donor R225. Z519 contained 16 substitution segments with 6.85 Mb of average length, which were distributed on 11 chromosomes of rice except the 10th chromosome. The bud sheath of Z519 began to appear the purple color stripes when it was about 3 mm long. Then the purple stripes displayed on sheaths, leaf margins, vascular bundles of stem and stigmas. While all parts of Nipponbare were green. Anthocyanin content in leaf sheath of Z519 was significantly higher than that of Nipponbare, whereas no significant difference was in flag leaf. Compared with Nipponbare, plant height of Z519 was significantly decreased, spikelets number and grain number of main panicle, and 1000-grain weight of Z519 were significantly increased. There was no significant difference between Z519 and Nipponbare in the other traits such as panicle number, main panicle length and seed-setting rate. Then, F2 recessive populations from the cross of Nipponbare and Z519 were used for genetic analysis and gene mapping of the purple sheath. The purple sheath in Z519 was controlled by a single dominant gene, named as PSH1, which was mapped on the chromosome 1 between InDel marker L03 and SSR marker L01 with the physical distance of 37.8 kb. By sequencing and gene-predicting in the region, Z519 had three bases (GTG) insertion in the GTG repeat area of the 238th?252th base in the first exon compared with Nipponbare, which resulted in increasing a Gly amino acid. Furthermore, the expression of LOC_Os01g45910 was obviously decreased in Z519 by qRT-PCR analysis. Thus, LOC_Os01g45910 was preliminary identified as the candidate gene of PSH1. The results lay a good foundation for studying molecular mechanisms of regulating anthocyanin by PSH1.

Key words: Rice (Oryza sativa L.), Chromosome segment substitution line (CSSL), Purple sheath PSH1, Gene mapping

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