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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (12): 2115-2122.doi: 10.3724/SP.J.1006.2013.02115

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Database Mining, Bioinformatics, Cloning and Expression Analyses of D Subfamily bZIP Genes in Maize

YANG Yan-Ge1,2,LÜ Wei-Tao2,SUN Dong-Mei1,*,LING Yi3,*,DENG Xin2,*   

  1. 1 College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2 Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; 3College of Life Science, China Agricultural University, Beijing 100193, China
  • Received:2013-04-22 Revised:2013-06-09 Online:2013-12-12 Published:2013-09-29
  • Contact: 孙冬梅,E-mail: sdmlzw@126.com,Tel:13089069957;凌毅,E-mail: lingyi01@cau.edu.cn,Tel:010-62734385;邓馨,E-mail: deng@ibcas.ac.cn,Tel:010-62836261

Abstract:

Basic leucine zipper (bZIP) is unique transcription factor family among eukaryotes, playing important roles in gene expression and regulation in higher plants. Here we reported the finding of 25 full length cDNA sequences encoding D subfamily of bZIP factors in maize from a database mining on whole genome level. The analysis of bioinformatics, chromosome distribution and classification of Possible Groups of Orthologous (PoGO) suggested the existence of diverse alternative splicing in some of these genes, which was supported by the sequencing results of the cloned cDNAs. The expression pattern of three representative genes in response to ABA and drought stress were examined by quantitative RT-PCR, and the results revealed different regulations of ABA on the expression of these genes. Our results suggest that D subfamily of bZIP genes in maize might involve in ABA signal pathway.

Key words: Maize, bZIP transcription factor, D subfamily, Database mining, Gene expression

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