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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (04): 525-531 .doi: 10.3724/SP.J.1006.2016.00525


Correlation Analysis between Total Catechins (or Anthocyanins) and Expression Levels of Genes Involved in Flavonoids Biosynthesis in Tea Plant with Purple Leaf

ZHOU Tian-Shan1,WANG Xin-Chao2,YU You-Ben1,XIAO Yao1,QIAN Wen-Jun1,XIAO Bin1*,YANG Ya-Jun2,*   

  1. 1.College of Horticulture, Northwest A&F University, Yangling 712100, China; 2.Key Laboratory of Tea Plant Biology and Resource Utilization, Ministry of Agriculture / Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2015-09-22 Revised:2016-01-11 Online:2016-04-12 Published:2016-01-19
  • Contact: 杨亚军, E-mail: yjyang@mail.tricaas.com; 肖斌, E-mail:xiaobin2093@sohu.com E-mail:zhoutianshan@nwsuaf.edu.cn
  • About author:杨亚军, E-mail: yjyang@mail.tricaas.com; 肖斌, E-mail:xiaobin2093@sohu.com
  • Supported by:

    This study was supported by the China Agriculture Research System (CARS-23).


Flavan-3-ols (aka catechins) and Anthocyanins found in large amounts in tea plant with purple leaf are synthesized through flavonoids metobolic pathway. To investigate the metobolic flux of flavanoids biosynthetic pathway in tea plant with purple leaf, we employed a tea plant with purple leaf and a tea plant with green leaf both from Mei-Tang-Tai-Cha, to examine the expression profiles of related genes involved in flavonoids biosynthesis intensively and determine the concentrations of catechins and anthocynin. Then correlation between total catechin (or anthocyanin) and expression levels of related genes was analyzed. The results indicated that the expression levels of related genes (PAL, CHS, CHI, F3H, DFR, ANS, ANR1, ANR2, F3’H,and F3’5’H) were up-regulated in purple leaves as compared with those in the green leaves. The purple leaf also had higher concentration of anthocyanin than the green leaf, while the green leaf was richer in total catechines. In purple leaves, the expression levels of related genes (except LAR) were highly correlated with both concentrations of total catechines (r = 0.84–0.99) and anthocyanin(r = 0.72–1.00). By contrast, there was only a high correlation between the expression levels of related genes (except LAR and F3¢H) and the concentration of total catechines (r = 0.64–0.77) in green leaves.

Key words: Tea plant with purple leaf, Gene expression analysis, Total catechins, Anthocyanins, Correlation analysis

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