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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 1059-1067.doi: 10.3724/SP.J.1006.2009.01059


Effects of Aluminum on Mitochondrial membrane Physiological Characteristics in Peanut Root Tips

ZHAN Jie1,KOU Rui-Jie1,LI Chuang-Zhen1,HE Hu-Yi2,HE Long-Fei1*   

  1. 1College of Agronomy,Guangxi University,Nanning 530005,China;2Institute of Economic Crops,Guangxi Academy of Agricultural Sciences,Nanning 530007,China
  • Received:2008-11-24 Revised:2009-02-17 Online:2009-06-12 Published:2009-04-16
  • Contact: HE Long-Fei,E-mail:lfhe@gxu.edu.cn


Mitochondria play a vital role in plant life. Peanut (Arachis hypogaea L.) culitivars LH11 (Al-resistant) and R1549 (Al-sensitive) were selected through root elongation experiment, hematoxylin dying and Al3+ concentration detection in root tips. The concentration of mitochondrial MDA in two cultivars root tips increased after Al3+ treatment that of R1549 was higher than that of LH11. The difference between two cultivars in mitochondrial MDA concentration was very significant in 20ΔΨ decreased significantly, mitochondrial Cyt c/a ratio reduced, which was more obvious in R1549 than in LH11 with Al3+ concentration increasing. To sum up, high Al3+ concentration treatment induced mitochondrial permeability transition pore opening, increased mitochondrial membrane permeability, decreased mitochondrial membrane Ca2+ transit system activity so that cytoplasm Ca2+ concentration increased, cytochrome c released into the cytoplasm, which might induce PCD(programmed cell death) in root tip, and inhibit root growth. Compared with Al-sensitive cultivar, Al-resistant cultivar has less Al3+ absorption and membrane lipid peroxidation level, higher control ability of Ca2+-ATPase activity, MPTP opening and ΔΨm maintaining so that is not easy to produce PCD under low Al3+ concentration stress. It may be one of reasons for Al resistance mechanism in plant.

Key words: Aluminum stress, Peanut(Arachis hypogaea L.), Roottip, Mitochondria, Permeability physiology

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