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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (12): 2285-2292.doi: 10.3724/SP.J.1006.2011.02285

• RESEARCH NOTES • Previous Articles     Next Articles

Mitochondrial Microsatellite Variability of Tobacco CMS Lines with Four Different Cytoplasms

中国农业科学院烟草研究所 / 中国农业科学院烟草遗传改良与生物技术重点开放实验室,山东青岛 266101   

  1. Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacoo Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China
  • Received:2011-05-23 Revised:2011-09-18 Online:2011-12-12 Published:2011-09-29
  • Contact: 孙玉合, E-mail: yhsun@163.com

Abstract: Sequence variation of the mitochondrial DNA has a direct relation with cytoplasmic male sterility (CMS). In this study, we analyzed mtSSR variability offour sterile cytoplasm types, including four CMS lines, their maintainer lines and sterial cytoplasm sources, and explored the relationship between mtSSR variability and CMS. Based on the entire mitochondrial DNA sequence of Nicotiana tabacum, we identified 39 mtSSR loci having more than ten mononucleotide repeats and 24 mtSSR markers that can be used to analyze mitochondrial DNA variability of four sterile cytoplasm types. The results showed that the mtSSR polymorphism was very low in N. tabacum, which was 10.5%, and there were some different loci between the CMS lines and their maintainer lines, the wild tobaccoprovided the sterile cytoplasm. Such as, in the Nat(sua.)S, the different loci were located in coding regions of orf138a, orf138c and intergenic region orf102–orf210; in the Nat(gla.)S, the different loci were located in two coding regions of orf138a, orf138c and four intergenic regionsnad2–sdh3, rps12–orf125b, orf116–orf101b, orf102–orf210; in the Nat(rep.)S, the different loci werelocated in coding regionorf190 and intergenic region orf104c–orf202; in the Nat(rus.)S, the different loci werelocated in coding regions oforf137 and rps12–orf125b. These different loci likely related to their CMS. The results also showed that the fragments of 184, 172, 212 and 225 bp were amplified by mtSSR primer TMS20 in four types of CMS of ms G-28, 86-6, 98-43, 116, respectively, and using this primer we could distinguish the four types of CMS of Nat(sua.)S, Nat(gla.)S, Nat(rep.)S, Nat(rus.)S between each other.

Key words: Tobacco, Sterile cytoplasm type, Mitochondrial DNA, SSR

[1]Xie J-P(谢剑平). Report on Advances in Tobacco Science and Technology 2009-2010 (烟草科学与技术学科发展报告2009—2010). Beijing: China Science and Technology Press, 2010. 9 (in Chinese )
[2]Bu G-Z(卜锅章), Gong M-L(龚明良), Ding C-M(丁昌敏), Yao S-M(姚绍满), Zhang L-L(张历历). A new strain of tobacco selected by somatic hybridizaion between N. tabacum and N. rustica. Chin Tab (中国烟草), 1992, (2): 1-5 (in Chinese)
[3]Kofer W, Glimelius K, Bonnett H T. Restoration of normal stamen development and pollen formation by fusion of different cytoplasmic male-sterile cultivars of Nicotiana tabacum. Theor Appl Genet, 1991, 81: 390-396
[4]Spangenberg G, Vicente R P, Oliveira M M, Osusky M, Nagel J, Pais M S, Potrykus I. CMS system in Nicotiana: flower development, patterns of mitochondrial DNA and mitochondrial gene expression. Sex Plant Reprod, 1992, 5: 13-26
[5]Tong D-R(佟道儒). Tobacco Breeding (烟草育种学), Beijing: China Agricultural Press, 1997. pp 284-288 (in Chinese)
[6]Sun Y-H(孙玉合), Ding C-M(丁昌敏), Zhang L-L(张历历), Bu G-Z(卜锅章), Gong M-L(龚明良). Breeding and use of a new tobacco CMS line 86-6. Acta Tab Sin (中国烟草学报), 1999, 5(1): 20-24 (in Chinese)
[7]Sun Y, Xue Q, Ding C, Wang W, Zhang L, Chen G, Han L, Wang Z. Somatic cybridizaion between Nicotiana tabacum and N. repanda based on a single inactivation procedure of nuclear donor parental protoplasts. Plant Sci, 2005, 168: 303-318
[8]Liu Q-Y(刘齐元), Liu F-H(刘飞虎), Yang J-B(杨建兵), Hou Y-P(候玉平), Liu M-Q(刘明求), Xiao F(肖伏). Study on stamen development of cytoplasmic male sterile line "Gexin 3" of tobacco (Nicotiana tabacum L). Acta Agric Univ Jiangxiensis (Nat Sci Edn) (江西农业大学学报•自然科学版), 2006, 28(2): 170-174 (in Chinese with English abstract)
[9]Liu Q-Y(刘齐元), Liu F-H(刘飞虎), Yang J-B(杨建兵), Hou Y-P(侯玉平), He H(何瀚). Cytological study on stamen development of cytoplasmic male sterile line “Yunyan 85” of tobacco (Nicotiana tabacum). Guihaia (广西植物), 2007, 27(2): 156-160 (in Chinese with English abstract)
[10]Chase C D. Cytoplasmic male sterility: a window to the world of plant mitochondrial-nuclear interactions. Trends Genet, 2007, 23: 81-90
[11]Rajender S, Rahul P, Mahdi A A. Mitochondria, spermatogenesis and male infertility. Mitochontrion, 2010, 10: 419-428
[12]Levings C S, Pring D R. Restriction endonuclease analysis of mitochondrial DNA from normal and Texas cytoplasmic male-sterile maize. Science, 1976, 193: 158-160
[13]Boeshore M L, Hanson M R, Izhar S. A variant mitochondrial DNA arrangement specific to Petunia stable sterile somatic hybrids. Plant Mol Biol, 1985, 4: 125-132
[14]Wang F, Feng C D, O'Connell M A, Stewart J M, Zhang J F. RFLP analysis of mitochondrial DNA in two cytoplasmic male sterility systems (CMS-D2 and CMS-D8) of cotton. Euphytica, 2010, 172: 93-99
[15]Hang W, Wang L, Yi P, Tan X-L, Zhang Z J, Zhu Y-G, Li Y S, Zhu Y G. RFLP analysis for mitochondrial genome of CMS-rice. Acta Genet Sin, 2006, 33: 330-338
[16]Sun L-Z(孙兰珍), Yao F-Y(姚方印), Li C-Y(李传友). RAPD analysing of CMS-T, K, V mtDNAs and cloning of mtDNA fragments associated with CMS-K in wheat. Acta Agron Sin (作物学报), 2001, 27(2): 144-148 (in Chinese with English abstract)
[17]Yi P, Wang L, Sun Q P, Zhu Y G. Discovery of mitochondria chimeric gene associated with male sterility of Honglian-rice. Chin Sci Bull, 2002, 47: 744-747
[18]Wei W-L(危文亮), Wang H-Z(王汉中), Liu G-H(刘贵华). Candidate sterility-related mitochondrial genes of NCa CMS in Brassica napus L. and its transcription regulated by restorer gene. Acta Agron Sin (作物学报), 2007, 33(1): 102-106 (in Chinese with English abstract)
[19]Bergman P, Edqvist J, Farbos I, Glimelius K. Male-sterile tobacco displays abnormal mitochondrial atp1 transcript accumulation and reduced floral ATP/ADP ratio. Plant Mol Biol, 2000, 42: 531-544
[20]Fittera J T, Thomas M R, Niu C, Rose R J. Investigation of Nicotiana tabacum (+) N. suaveolens cybrids with carpelloid stamens. J Plant Physiol, 2005, 162: 225-235
[21]Zhao T(赵婷), Zhu T-Y(朱滕义), Liu Q-Y(刘齐元), Zhang M-L(张美良), Jiang H-Y(蒋海燕). SNP in tobacco mitochondrial gene atp6 and its correlation with CMS. Acta Agron Sin (作物学报), 2009, 35(9): 1655-1661 (in Chinese with English abstract)
[22]Zhu T-Y(朱腾义), Fan D-D(范东东), Zhao T(赵婷), Liu Q-Y(刘齐元), Wang J-G(王建革), Zhu X-W(朱肖文), Cheng Y-Q(程元强). SNP detection in tobacco (Nicotiana tabacum) mitochondrial gene CoxII and its association with CMS. J Nucl Agric Sci (核农学报), 2010, 24(4): 720-727 (in Chinese with English abstract)
[23]Liu Q-Y(刘齐元), Liu F-H(刘飞虎), Huang H-Q(黄海泉), He K-X(何宽信), Xiao F(肖伏). RAPD analysis of mitochondrial DNAs of cytoplasmic male sterile line and its maintainer in tobacco (Nicotiana tobacum). Acta Agric Univ Jiangxiensis (Nat Sci Edn)(江西农业大学学报•自然科学版), 2005, 27(6): 826-830 (in Chinese with English abstract)
[24]Shakyawar S K, Joshi B K, Kumar D. SSR repeat dynamics in mitochondrial genomes of five domestic animal species. Bioinformation, 2009, 15: 158-163
[25]Ishii T, Takahashi C, Ikeda N, Kamijima O, Mori N. Mitochondrial microsatellite variability in common wheat and its ancestral species. Genes Genet Syst, 2006, 81: 211-214
[26]Hosaka K, Sanetomo R. Comparative differentiation in mitochondrial and chloroplast DNA among cultivated potatoes and closely related wild species. Genes Genet Syst, 2009, 84: 371-378
[27]Yan N(燕妮). SSR analysis of mitochonrial DNA of some sterile lines of rapeseed. MS Dissertations of Gansu Agricultural University, 2007. pp 1-40
[28]Hao C-Y(郝晨阳), Wang L-F(王兰芬), Jia J-Z(贾继增), Dong Y-C(董玉琛), Zhang X-Y(张学勇). Comparison of fluorescence and silver-staining detection systems of microsatellite markers. Acta Agron Sin (作物学报), 2005, 131: 144-149 (in Chinese with English abstract)
[29]Castillo A, Budak H, Varshney R K, Dorado G, Graner A, Hernandez P. Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense. BMC Plant Biol, 2008, 8: 97-105
[30]Studer B, Asp T, Frei U, Hentrup S, Meally H, Guillard A, Barth S, Muylle H, Roldán-Ruiz I, Barre P, Koning-Boucoiran C, Uenk-Stunnenberg G, Dolstra O, Skøt L, Skøt K P, Turner L B, Humphreys M O, Kölliker R, Roulund N, Nielsen K K, Lübberstedt T. Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). Mol Breed, 2008, 21: 533-548
[31]Simko I. Development of EST-SSR markers for the study of population structure in lettuce (Lactuca sativa L.). J Hered, 2009, 100: 256-262
[32]Sharma V, Bhardwaj P, Kumar R, Sharma R K, Sood A, Ahuja P S. Identification and cross-species amplification of EST derived SSR markers in different bamboo species. Conserv Genet, 2009, 10: 721-724
[33]Sugiyama Y, Watase Y, Nagase M, Makita N, Yagura S, Hirai A, Sugiura M. The complete nucleotide sequence and multipartite organization of the tobacco mitochondrial genome: comparative analysis of mitochondrial genomes in higher plants. Mol Gen Genomics, 2005, 272: 603-615
[34]Tong D-R(佟道儒), Niu P-L(牛佩兰), Luo Q-Z(骆启章), Bu G-Z(卜锅章). Study on breeding and utilization of a tobacco male sterile line. Chin Tab (中国烟草), 1980, 2: 5-8 (in Chinese)
[35]Li W-Q(李文强), Zhang G-S(张改生), Wang K(汪奎), Niu N(牛娜), Pang D(潘栋). An efficient method for isolation of mitochondrial DNA in wheat. Herditas (遗传), 2007, 29(6): 771-775 (in Chinese with English abstract)
[36]Bland M M, Matzinger D F, Levings C S. Comparison of the mitochondrial genome of Nicotiana tabacum with its progenitorspecies. Theor Appl Genet, 1985, 69: 535-541
[37]Li S, Tan Y, Wang K, Wan C, Zhu Y. Gametophytically alloplasmic CMS line of rice (Oryza sativa L.) with variant orfH79 haplotype corresponds to specific fertility restorer. Theor Appl Genet, 2008, 117: 1389-1397
[38]Li S-S(李双双), Xue L-F(薛龙飞), Su A-G(苏爱国), Lei B-B(雷彬彬), Wang Y-M(王玉美), Hua J-P(华金平). Progress on sequencing and alignment analysis of higher plant mitochontrial genomes. J China Agric Univ (中国农业大学学报), 2011, 16: 22-27 (in Chinese with English abstract)
[39]Wolfe K H, Li W-H, Sharp P M. Rates of nucleotide substitution vary greatly among plant mitochondrial, cholroplast, and nuclear DNAs. Proc Natl Acad Sci USA, 1987, 84: 9054-9058
[40]Kubo T, Newton K J. Angiosperm mitochondrial genomes and mutations. Mitochondrion, 2008, 8: 5-14
[41]Cho Y, Mower J P, Qiu Y L, Palmer J D. Mitochondrial substitution rates are extraordinarily elevated and variable in a genus of flowering plants. Proc Natl Acad Sci USA, 2004, 101: 17741-17746
[42]Mouse S V. Examining rates and patterns of nucleotide subtitution in plants. Plant Mol Biol, 2000, 42: 25-43
[43]Clarkson J J, Kelly L J, Leitch A R, Knapp S, Chase M W. Nuclear glutamine synthetase evolution in Nicotiana: phylogenetics and the origins of allotetraploid and homoploid (diploid) hybrids. Mol Phylogenet Evol, 2010, 55: 99-112
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