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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (11): 2184-2198.doi: 10.3724/SP.J.1006.2021.04240


Identification and analysis of non-specific lipid transfer protein family in tobacco

LI Peng1(), LIU Che1, SONG Hao1, YAO Pan-Pan1, SU Pei-Lin1, WEI Yao-Wei1, YANG Yong-Xia1,*(), LI Qing-Chang2,*()   

  1. 1Tobacco College, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, Henan, China
  • Received:2020-11-05 Accepted:2021-04-26 Online:2021-11-12 Published:2021-05-13
  • Contact: YANG Yong-Xia,LI Qing-Chang E-mail:lpeng1995@126.com;yyx624@126.com;ctsrc@126.com
  • Supported by:
    Henan Province Science and Technology Research Plan (Agricultural Field) Project(182102110315);Henan Province Science and Technology Research Plan (Agricultural Field) Project(192102110002);Henan Province Science and Technology Research Plan (Agricultural Field) Project(192102110121);Henan University Student Innovation and Entrepreneurship Training Program(S202010466013);Open Innovation Project of Undergraduate Laboratory of Henan Agricultural University(KF1908)


Plant non-specific lipid transfer proteins (nsLTPs) can transfer lipids in vitro, regulate plant growth and development, and respond to environmental abiotic and biotic stresses. In this study, 74 nsLTPs genes were identified from the genome of Nicotiana tabacum variety K326, and we analyzed multiple characteristics of these genes, including phylogeny, gene structures, conserved motifs, protein domains, chromosome locations, cis-elements in the promoter sequences, 3D structure, and the expression patterns under different hormones and abiotic stresses. The results revealed that nsLTPs in tobacco could be divided into eight types, including type I, II, III, IV, V, VII, VIII, and XIII, according to the interval and sequence similarity between the eight cysteines. The same types of NtLTPs had similar intron-exon patterns and conserved motifs, motif 2 and motif 3 were the characteristic motifs of NtLTPs family. In the process of evolution, fragment duplication dominated the expansion of the NtLTPs family. RNA-seq analysis after drought treatment revealed that the functional differentiation patterns of repeat gene pairs were diverse during evolution period. Promoter analysis showed that they contained a variety of cis-acting elements in response to light response, hormones, and abiotic stress. Furthermore, qRT-PCR demonstrated that NtLTPs family genes had different expression patterns in different tissues and organs of tobacco plants, which could respond to abiotic stresses such as drought, salt, and hormone treatments (IAA, GA, and SA etc.). These results provide a theoretical reference for the in-depth analysis of the functions of NtLTPs family genes and molecular breeding.

Key words: tobacco, nsLTPs, gene family, bioinformatics, gene expression

Table 1

Primers used in this study"

引物名称Primer name 引物序列Primer sequence (5′-3′)
Nitab4.5_0000125g0010-F CGGATCGCCGGAGTGTTTGC
Nitab4.5_0000125g0010-R GCCACATTTGCCAGGGAGGG
Nitab4.5_0004362g0040-F AAGCCGATTTGCGTTGTATG
Nitab4.5_0004362g0040-R CTTAGGCAGTTTCATAGCAG

Table 2

Diversity of eight-cysteine motifs (ECM) of nsLTPs in tobacco"

Number of members
I 38 C X9,24 C X12-16 CC X19,20,22 CXC X21-23 C X10-15,25 C
II 6 C X7 C X13 CC X8 CXC X23 C X6,10 C
III 1 C X9 C X14 CC X9 CXC X12 C X6 C
IV 10 C X9,10 C X14-16 CC X9,12 CXC X24 C X7-10 C
V 1 C X14 C X14 CC X11 CXC X24 C X10 C
VII 5 C X9 C X14,16 CC X12 CXC X25,27 C X9 C
VIII 7 C X6 C X12,14 CC X12 CXC X25,27 C X8 C
XIII 6 C X9 C X14 CC X12 CXC X26,30 C X8 C

Fig. 1

Distributions of molecular weights and isoelectric points of tobacco nsLTPs"

Fig. 2

Phylogenetic relationship of nsLTPs family in tobacco"

Fig. 3

Conserved motifs of tobacco nsLTPs proteins (middle part) and gene structures of nsLTPs genes (right part) in tobacco"

Fig. 4

Conserved cysteine domain of nsLTPs in tobacco"

Fig. 5

Distribution of nsLTPs genes on the chromosomes in tobacco"

Fig. 6

Gene duplications of nsLTPs genes in tobacco"

Fig. 7

Frequency distribution of Ka and Ka/Ks values of duplicated NtLTPs gene pairs"

Fig. 8

3D-structure of nine nsLTPs proteins in tobacco"

Fig. 9

Cis-acting elements of nsLTPs promoter in tobacco"

Fig. 10

Relative expression profile of variety K326 under drought treatment in tobacco The relative expression levels of nsLTPs are measured by FPKM value in RNA-seq data and the relative expression heatmap are drew after FPKM normalized by log2. CK represents no drought treatment, and D represents drought treatment. Red and white color indicate high and low expression levels, respectively."

Fig. 11

Relative expression levels of Nitab4.5_0000125g0010 (type I) and Nitab4.5_0004362g0040 (type IV) in different tissues of tobacco The relative expression levels in the roots were used as controls, with high and low relative expression levels in red and green, respectively. R: root; ST: stem; L: lugs; ML: middle leaf; UL: upper leaf; F: flower."

Fig. 12

Relative expression patterns of Nitab4.5_0000125g0010 (black) and Nitab4.5_0004362g0040 (gray) under different treatments The relative gene expression was calculated by 2-ΔΔCT method. The relative expression of 0 h was set to 1. Error bars represent the standard deviations of three biological replicates. 37°C: high temperature treatment; NaCl: salt treatment; Mannitol: mannitol treatment; GA: gibberellin treatment; IAA: auxin treatment; MeJA: methyl jasmonate treatment; ABA: abscisic acid treatment; SA: salicylic acid treatment. *: P < 0.05."

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