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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (05): 754-763.doi: 10.3724/SP.J.1006.2010.00754


Mining Elite Alleles for Seed Vigor Traits in Two Populations of Japonica Rice (Oryza sativa L.) in Taihu Lake Region

WANG Yang1,2,GUO Yuan1,3,HONG De-Lin1,*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Department of Agricultural Resource and Environment, Heilongjiang University, Harbin 150008, China; 3 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
  • Received:2009-08-22 Revised:2009-12-19 Online:2010-05-12 Published:2010-03-03
  • Contact: HONG De-Lin,E-mail:delinhong@njau.edu.cn


Direct seeding in rice (Oryza sativa L.) is a labor-saving, high efficient and water-saving cultivation pattern. Lower temperature stress is the main factor affecting seedling establishment in direct seeding rice field. Breeding rice cultivars with high seed vigor is one of the ways to improve seedling establishment. In order to provide genetic information and breeding materials for breeding cultivars suitable to direct sowing in japonica rice, we conducted this research. Root length, shoot height, and dry weight of seedlings germinated for 7 days on the sloped board were investigated in a natural population composed of 94 varieties from Taihu Lake Region and a recombinant inbred line (RIL) population derived from a cross between Xiushui 79 and C Bao in japonica rice (Oryza sativa L.). QTL analysis of seed vigor was performed by using General Linear Model program in Tassel software for the natural population and Composite Internal Mapping program in Win QTL Cartographer 2.5 software for the RIL population. Elite alleles and their carrier materials were determined on the basis of the result of QTL analysis. The results obtained were as follows: (1) In the natural population, eleven SSR marker loci associated with seed vigor were detected. Forty-two elite alleles for seed vigor traits were detected in the natural population. Among them, 17 associated with root length, 13 with shoot height and 12 with dry weight. Diantun 502 xuanzao, Yangdao 6, Kaiqing, Xianhui 429 and C Bao were carrier materials for the elite alleles of seed vigor. (2) In the RIL population, nine QTLs, including two for root length, four for shoot height and three for dry weight, were detected. All the elite alleles came from C Bao except RM525-143 bp, which associated with dry weight and came from Xiushui 79. (3) In both populations, SSR marker locus associated with root length was detected on chromosome 1, SSR marker loci associated with shoot height were detected on chromosomes 2, 8 and 11, and SSR marker locus associated with dry weight was detected on chromosome 2. More or better elite alleles were detected in the natural population than in the RIL population.

Key words: Japonica rice(Oryza sativa L.), Seed vigor, Elite alleles, Natural population, Family population

[1]Wang C-L(王才林), Zou J-S(邹江石), Tang L-H(汤陵华), Ding J-L(丁金龙), Zhang M(张敏), Zhou Y-X(周裕兴), Udatsu T(宇田津彻朗), Fujiwara H(藤原宏志). Rice cultivation at the Neolithic age in Taihu valley. Jiangsu J Agric Sci (江苏农业学报), 2000, 16(3): 129-138 (in Chinese with English abstract)
[2]Jin W-D(金伟栋), Hong D-L(洪德林). Genetic diversity in japonica rice landraces (Oryza sativa) from the Taihu lake region
[J]. Biodiversity Science (生物多样性.2006, 14(6):479-487
[3]Jin W-D(金伟栋), Hong D-L(洪德林). Establishment of core collections of japonica rice landraces (Oryza sativa L.) in Tai Lake Valley. Jiangsu J Agric Sci (江苏农业学报), 2007, 23(6): 516-525 (in Chinese with English abstract)
[4]Jin W-D(金伟栋), Cheng B-S(程保山), Hong D-L(洪德林). Genetic diversity analysis of japonica rice landraces (Oryza sativa L.) in Tai Lake Region based on SSR markers. Sci Agric Sin (中国农业科学), 2008, 41(11): 3822-3830 (in Chinese with English abstract)
[5]Wu W-G(吴文革), Chen Y(陈 烨), Qian Y-F(钱银飞), Wang X-J(王小军). The Current status and progresses of the research on direct seeding rice
[J].Rev China Agric Sci & Technol (中国农业科技导报.2006, 8(4):32-36
[6]Yamauchi M, Aguilar A M, Vaughan D A. Rice (Oryza sativa L.) germplasm suitable for direct sowing under flooded soil surface. Euphytica, 1993, 67: 177-184
[7]Wang Y(王洋), Guo Y(郭媛), Hong D-L(洪德林). QTL analysis of the anoxic tolerance at the seedling stage in rice (Oryza sativa L.). Chin J Rice Sci (中国水稻科学), 2010, 24(1): 18-24
[8]Zhang Z H, Yu S B, Yu T, Huang Z, Zhu Y G. Mapping quantitative trait loci (QTLs) for seedling-vigor using recombinant inbred lines of rice (Oryza sativa L
[J].). Field Crops Res
[9]Cao L-Y(曹立勇), Zhu J(朱军), Ren L-F(任立飞), Zhao S-T(赵松涛), Yan Q-C(颜启传). Mapping QTLs and epistasis for seedling vigor in rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2002, 28(6): 809-815 (in Chinese with English abstract)
[10]Huang Z, Yu T, Su L, Yu S B, Zhang Z H, Zhu Y G. Identification of chromosome regions associated with seedling vigor in rice. Acta Genet Sin, 2004, 31(6): 596-603
[11]Cui K H, Peng S B, Xing Y Z, Xu C G, Yu S B, Zhang Q. Molecular dissection of seedling-vigor and associated physiological traits in rice
[J].Theor Appl Genet
[12]March R E. Gene mapping by linkage and association analysis
[J].Mol Biotechnol
[13]Zhang N, Xu Y, Akash M, McCouch S, Oard J H. Identification of candidate markers associated with agronomic traits in rice using discriminant analysis
[J].Theor Appl Genet
[14]Breseghello F, Sorrells M S. Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics, 2006, 172: 1165-1177
[15]Wang Y(王洋), Wang Y-Y(王盈盈), Hong D-L(洪德林). Genetic variation of seed vigor and tolerance to anoxia among rice (Oryza sativa L.) accessions in Tai lake region. J Nanjing AgricUniv (南京农业大学学报), 2009, 32(3): 1-7 (in Chinese with English abstract)
[16]Temnykh S, Park W D, McCouch S R. Mapping and genome organization of microsatellite sequence in rice (Oryza sativa L
[J].). Theor Appl Genet
[17]McCouch S R, Teytelman L, Xu Y. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L
[J].). DNA Res
[18]Guo Y(郭媛), Chen B-S(程保山), Hong D-L(洪德林). Construction of SSR linkage map and analysis of QTL for rolled leaf of restorer line in japonica rice (Oryza sativa L.). Chin J Rice Sci (中国水稻科学), 2009, 23(3): 245-251 (in Chinese with English abstract)
[19]Churchill G A, Doerge R W. Empirical threshold values for quantitative trait mapping. Genetics, 1994, 138: 963-971
[20]McCouch S R, Cho Y G, Yano M. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11-13
[21]Gai J-Y(盖钧镒). Methods of Experimental Statistics (试验统计方法). Beijing: China Agriculture Press, 2000. pp 35-125 (in Chinese)
[22]Eizenga G C, Agrama H A, Lee F N, Yan W, Jia Y. Identifying novel resistance genes in newly introduced blast resistant rice germplasm
[J].Crop Sci
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