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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2020-2027.doi: 10.3724/SP.J.1006.2010.02020


Genetic Analysis of Homozygous-Dominant-Surviving Ligon Lintless Recombinants in Cotton (Gossypium hirsutum L.)

LIU Feng-Ju,LIANG Wen-Hua,ZHANG Tian-Zhen*   

  1. National Key Laboratory of Crop Genetics & Germplasm Enhancement / Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-04-13 Revised:2010-07-28 Online:2010-12-12 Published:2010-10-09
  • Contact: ZHANG Tian-Zhen, E-mail: cotton@njau.edu.cn, Tel: 025-84395037

Abstract: The Li1 mutant discovered by Griffee and Ligon in 1929 is characterized by its short lint fiber, contorted leaf laminae and a twisted appearance of the branches and stems. It was observed that the homozygous mutant plants appeared slightly smaller than the heterozygous ones. Li1 mutant has never been observed to produce homozygous Li1 phenotype by selfing since it was introduced and planted in China. To study the allelic relationship between three fiberless mutant (XZ142 FLM,MD17, and SL1-7-1) genes and Li1 gene, we crossed the Li1 mutant with these three fiberless mutants, respectively. From the cross Li1 × XZ142 FLM, we unexpectedly got a recombinant with all distorted leaves and short lint fibers. And there was no segregation in their F2 progeny, which indicated that the mutation is homozygous of Li1 gene. We named this homozygous Li1 mutantas Ligon lintless recombinant (Li-R). Segregating populations were produced to conduct the inheritance analysis of homozygous-dominant- surviving phenotype of this Ligon lintless recombinant Li-R. Segregation of four F2s derived from Li-R× XZ142 FLM, Li-R×TM-1, Li-R×Hai 7124, and Li-R×Li1 indicated that the segregation of the homozygous-dominant-surviving phenotype of Li-Rfit a two locus inheritance model, one locus being the dominant Li1 and another a new recessive locus derived from XZ142 FLM, and we proposed to name it as lia. Therefore, the genotype of homozygous-dominant-surviving plant Li-Ris lialiaLi1Li1, and that of homozygous-dominant-leathal plant of Li1 is LiaLiaLi1Li1, the genotype of XZ142 FLM is lialiali1li1, that of the heterozygousLi1 mutant found in 1929 is LiaLiaLi1li1. Segregation analysis of F2-derived F3 families from Li-R×TM-1 revealed that lia is non-allalic to li3 and n2, both genes leading to fiberless seed phenotype.

Key words: Ligon lintless recombinant, Homozygous-dominant-survive, Recessive allele

[1]Griffee F, Ligon L L. Occurrence of lintless cotton plants and the inheritance of the character ‘lintless’. J Amer Soc Agron, 1929, 21: 711–717
[2]Kohel R J. Linkage tests in upland cotton, Gossypium hirsutum L. II. Crop Sci, 1972, 12: 66–69
[3]Narbuth E V, Kohel R J. Inheritance and linkage analysis of a new fiber mutant in cotton. J Hered, 1990, 81 : 131–133
[4]Karaca M, Saha S, Jenkins JN, Zipf A, Kohel R, Stelly D M. Simple sequence repeat (SSR) markers linked to the Ligon lintless (Li1) mutant in cotton. J Hered, 2002, 93: 221–224
[5]Rong J K, Pierce G, Waghmare V, Rogers C, Desai A, Chee P, May O, Gannaway J, Wendel J, Wilkins T. Genetic mapping and comparative analysis of seven mutants. Theor Appl Genet, 2005, 111: 1137–1146
[6]Pan J-J(潘家驹). Cotton Breeding (棉花育种学). Beijing: China Agriculture Press, 1998. pp 78–79(in Chinese)
[7]Zhang T-Z(张天真), Pan J-J(潘家驹). Genetic analysis of fuzzless-lintless mutant in upland cotton. J Jiangsu Agric Sci (江苏农业学报), 1991, 7: 13–16 (in Chinese with English abstract)
[8]Kohel R J, Stelly D M, Yu J. Tests of six cotton (Gossypium hirsutum L.) mutants for association with aneuploids. J Hered, 2002, 93: 130–132
[9]Turley R B. Registration of MD17 fiberless upland cotton as a genetic stock. Crop Sci, 2002, 42: 994–995
[10]Turley R B, Kloth R H. The inheritance model for the fiberless trait in upland cotton (Gossypium hirsutum L.) line SL1-7-1: variation on a theme. Euphytica, 2008, 164: 123–132
[11]Ding Y-Z(丁业掌), Guo W-Z(郭旺珍), Zhang T-Z(张天真). Genetic analysis of two mutants of fiber in Gossypium hirsutum L. Cotton Sci Sin (棉花学报), 2007, 19(3): 179–182 (in Chinese with English abstract)
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