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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 842-854.doi: 10.3724/SP.J.1006.2011.00842

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Nitrogen Management on Protein Expression of Flag Leaves during Grain-Filling Period in Large Panicle Rice (Oryza sativa L.)

ZHANG Zhi-Xing1,2,LI Zhong1,2,CHEN Jun1,2,LI Qi-Song1,2,CHEN Long-Huai1,2,CHEN Hong-Fei1,2,HUANG Jin-Wen1,LIN Wen-Xiong1,2,*   

  1. 1 Institute of Agricultural Ecology, Fujian Agricultural and Forestry University, Fuzhou 350002, China; 2 School of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China
  • Received:2010-10-28 Revised:2011-03-08 Online:2011-05-12 Published:2011-03-24
  • Contact: 林文雄, E-mail: wenxiong181@163.com, Tel: 0591-83769440

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Abstract: In rice (Oryza sativa), approximately 60%–100% of the carbon in mature grains originates from CO2 assimilation during the grain-filling period, with the flag leaf as the most important contributor to the yield. It is therefore important to understand molecular mechanisms of flag leaf during the grain-filing period. Two-dimensional electrophoresis (2-DE) technology was applied to investigate the differential expression patterns of leaf proteins from different periods during grain-filling process in rice cultivation Jinhui 809 treated by two different nitrogen application ratios. The results showed 32 differentially expressed proteins with 27 up-regulated and five down-regulated, in response to increased nitrogen application at grain-filling stage. According to their relative functions the identified proteins were classified into five main categories: photosynthesis (12); adversity-resistance response (5); hormone synthesis and signal transduction (5); cell growth and differentiation (5); unknown functions (5). Then photosynthesis-related features together with adversity-defense related indicators (SOD, POD, CAT, MDA, and soluble protein) were determined. The appropriate increase in nitrogen application at late growth stage on one hand delayed the degradation of chlorophyll and soluble protein in leaf during grain filling period, in turn elongated the leaf photosynthesis process; on the other hand promoted the activities of SOD, POD, CAT, while decreased the lipid peroxidation degree, which together indicated the strengthen of leaf adversity-defense ability. The results not only verify the high efficient effect of increased nitrogen application on leaf metabolisms during grain-filling process, but also provide the theoretic basis for studies on rational management of nitrogen fertilizer and its relevant molecular regulation in the future.

Key words: Rice, Grain-filling, Flag leaf, Protein, Nitrogen fertilizer

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