Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (01): 157-162.doi: 10.3724/SP.J.1006.2008.00157


Effect of HgCl2 Short Time Treatment on Photosynthesis in Broad Bean Leaves

WANG Hong-Wei1,SHI Ya-Qi1,HUANG Feng1,YUAN Lin2   

  1. 1 School of Resources and Environmental Engineering, Wuhan University of Technology; 2 Biomedical Materials and Engineering Center, Wuhan University of Technology, Wuhan 430070, Hubei, China

  • Received:2007-03-26 Revised:1900-01-01 Online:2008-01-12 Published:2008-01-12
  • Contact: WANG Hong-Wei


Mercury pollution is a major environmental problem. Unlike most heavy metals, plants uptake mercury primarily through leaves rather than the root system. Most of the mercury existed as complexes of Hg2+, which could cause the inhibition of photosynthesis as well as plant growth in higher plants. Since photosynthesis is very sensitive to heavy metals in higher plants, green algae and cyanobacteria, the inhibition of photosynthesis by Hg2+ had been examined in CO2 fixation and photosystems activities. However, in most of the researches, Hg2+ solutions were applied in vitro or not directly on plant leaves, the mechanism of inhibition on photosynthesis by Hg2+ is still in controversy. In order to discover its effect on leaf photosynthesis, HgCl2 solutions were applied to intact broad bean (Vicia faba L.) leaves. In the experiment, Hg2+ contents in intact broad bean leaves were determined after 30 min treatment with different concentrations of HgCl2 solution. And leaf gas exchange and chlorophyll fluorescence were measured under a definite light intensity and CO2 concentration. The results showed that the Hg content in leaves increased with the increase of treated Hg concentrations at the range from 0.5 mg L-1 to 2 000 mg L-1. When the applied HgCl2 concentration was 0.5 mg L-1, the increase of leaf mercury content compared with that of control was less than 20%. If the applied HgCl2 increased to 500 mg L-1, Hg content in leaves was about 800% of control. Meanwhile, leaf net photosynthetic rate (Pn) was unaffected at the treated HgCl2 concentrations from 0.5 mg L-1 to 5.0 mg L-1, and gradually decreased with the increase of treated Hg concentrations from 10 mg L-1 to 2 000 mg L-1. The inhibition of leaf’s Pn was about 36% after 2000 mg L-1 HgCl2 treatment. The results suggested that broad bean could absorb mercury solution through the leaves and thus lead to the inhibition of leaf photosynthetic activities.
Further studies showed that both leaf’s apparent quantum yield (AQY) and electron transport rate (ETR) were decreased significantly, with the increase of HgCl2 concentrations. Moreover, the quantum yield of PSⅡ(DF/Fm′) were inhibited whereas the non-photochemical quenching (NPQ) was increased by HgCl2 concentrations from 20 mg L-1 to 2 000 mg L-1, indicating that the decrease of Pn in broad bean leaves by low concentrations of HgCl2 solutions short time treatment might be mainly due to the inhibition of photosynthetic electron transport caused by the decrease of light energy transferring to active center and the increase of the light energy dissipation in PSⅡ.

Key words: Mercuric chloride (HgCl2), Net photosynthetic rate (Pn), Apparent quantum yield (AQY), Chlorophyll fluorescence

[1] LI Jing, WANG Hong-Zhang, LIU Peng, ZHANG Ji-Wang, ZHAO Bin, REN Bai-Zhao. Differences in photosynthetic performance of leaves at post-flowering stage in different cultivation modes of summer maize (Zea mays L.) [J]. Acta Agronomica Sinica, 2021, 47(7): 1351-1359.
[2] JING Xia, ZOU Qin, BAI Zong-Fan, HUANG Wen-Jiang. Research progress of crop diseases monitoring based on reflectance and chlorophyll fluorescence data [J]. Acta Agronomica Sinica, 2021, 47(11): 2067-2079.
[3] BAI Zong-Fan,JING Xia,ZHANG Teng,DONG Ying-Ying. Canopy SIF synergize with total spectral reflectance optimized by the MDBPSO algorithm to monitor wheat stripe rust [J]. Acta Agronomica Sinica, 2020, 46(8): 1248-1257.
[4] HOU Hong-Qian,LIN Hong-Xin,LIU Xiu-Mei,JI Jian-Hua,LIU Yi-Ren,LAN Xian-Jin,LYU Zhen-Zhen,ZHOH Wei-Jun. Influence of long-term fertilizer application on chlorophyll fluorescence characteristics and grain yield of double cropping late rice [J]. Acta Agronomica Sinica, 2020, 46(02): 280-289.
[5] Yang GAO,Wei-Guo LIU,Shu-Xian LI,Ting LIU,Tao ZHOU,Yong-Li DU,Yi ZHANG,Bi-Qin LI,Wen-Yu YANG. Effect of shade priming on photosynthetic characteristics of soybean seedlings [J]. Acta Agronomica Sinica, 2019, 45(1): 91-99.
[6] Qing-Fei WU, Lei QIN, Lei DONG, Ze-Hong DING, Ping-Hua LI, Bai-Juan DU. Transcriptome Analysis on a Maize Photosynthetic Mutant hcf136 (high chlorophyll fluorescence 136) [J]. Acta Agronomica Sinica, 2018, 44(04): 493-504.
[7] WANG Shun-Yi,LI Huan,LIU Qing,SHI Yan-Xi*. Effect of Potassium Application on Root Grow and Yield of Sweet Potato and Its Physiological Mechanism [J]. Acta Agron Sin, 2017, 43(07): 1057-1066.
[8] YANG Chuan-Bang,YU Zhen-Wen,ZHANG Yong-Li*,SHI Yu. Effect of Soil Depth with Supplemental Irrigation on Canopy Photosynthetically Active Radiation Interception andChlorophyll Fluorescence Parameters in Jimai 22 [J]. Acta Agron Sin, 2017, 43(02): 253-262.
[9] LIU Ling-Yun,LIU Hao,ZHAO Jing,WANG Yan-Xia,WANG Peng-Tao*. Map-based Cloning and Functional Analysis of Low Chlorophyll Fluorescence Gene LCF3 in Arabidopsis thaliana [J]. Acta Agron Sin, 2016, 42(05): 690-695.
[10] JU Cheng-Xin,TAO Jin,QIAN Xi-Yang,GU Jun-Fei,ZHANG Hao,ZHAO Bu-Hong,LIU Li-Jun,WANG Zhi-Qin,YANG Jian-Chang. Leaf Photosynthetic Characteristics of Mid-season Indica Rice Varieties Applied at Different Decades [J]. Acta Agron Sin, 2016, 42(03): 415-426.
[11] YE Lu-Huan,SHEN Wei-Jun,ZHENG Bao-Gang,SONG Tao,CHEN Guo-Xiang,Lü Chuan-Gen. Changes of Photosynthetic Membrane Function and Protein Complexes in Flag Leaves of Liangyoupeijiu during Leaf Senescence [J]. Acta Agron Sin, 2013, 39(11): 2030-2038.
[12] DU Wei-Li,GAO Jie,HU Fu-Liang,GUO De-Lin,ZHANG Gai-Sheng,ZHANG Ren-He*,XUE Ji-Quan. Responses of Drought Stress on Photosynthetic Trait and Osmotic Adjustment in Two Maize Cultivars [J]. Acta Agron Sin, 2013, 39(03): 530-536.
[13] XIAO Hua-Gui,YANG Huan-Wen,RAO Yong,YANG Bin,ZHU Ying. Photosynthetic Characteristics and Chlorophyll Fluorescence Kinetic Parameters Analyses of Chlorophyll-Reduced Mutant in Brassica napus L. [J]. Acta Agron Sin, 2013, 39(03): 520-529.
[14] WANG Xiao-Lei,YU Hai-Qiu,LIU Ning,YI Bing,CAO Min-Jian. Physiological Characteristics of Delaying Leaf Senescence in Maize Inbred Lines Tolerant to Potassium Deficiency [J]. Acta Agron Sin, 2012, 38(09): 1672-1679.
[15] FENG Xiao-Min,ZHANG Yong-Qing. Effect of Water Stress on Seedling Growth and Photosynthetic Characteristics in Broomcorn Millet [J]. Acta Agron Sin, 2012, 38(08): 1513-1521.
Full text



No Suggested Reading articles found!