玉米光合突变体hcf136 (high chlorophyll fluorescence 136)的转录组分析

doi:10.3724/SP.J.1006.2018.00493

Abstract

Abstract:

Maize (Zea mays) is a typical C₄ plant, which photosynthetic activities are partitioned by the cooperating of Kranz anatomy—mesophyll (M) cell and bundle sheath (BS) cell. The maize high chlorophyll fluorescence 136 (hcf136) mutant is an ideal material for C₄ photosynthesis study, due to it lacks PSII activity without normal thylakoid grana in M chloroplasts. However, the development of BS chloroplasts is not affected. In this study, we utilized RNA-Seq technology to monitor the transcriptome changes in different leaf sections between wild type and hcf136 mutant under different light intensities. The results indicated that the impairment of PSII is not caused by the transcription changes of PSII related genes. We also noticed that the starch biosynthesis was blocked in the mutant; and the sugar degradation, sugar transport and copper ion transport were triggered. In addition, the expression level of transcription factors was changed distinctly. These results provide valuable information for understanding a comprehensive function of HCF136 gene in future.

Key words: Zea mays, high chlorophyll fluorescence 136 mutant, RNA-Seq, C₄ photosynthesis

Qing-Fei WU, Lei QIN, Lei DONG, Ze-Hong DING, Ping-Hua LI, Bai-Juan DU. Transcriptome Analysis on a Maize Photosynthetic Mutant hcf136 (high chlorophyll fluorescence 136)[J].Acta Agronomica Sinica, 2018, 44(04): 493-504.

Figures/Tables 9

Fig.1

Table 1

Fig. 2

Table 2

Differentially expressed genes in wild type (WT) and mutant (hcf136) in both -1 cm and +4 cm under both high and low light treatments"

基因编号 Gene ID	基因注释 Gene annotation	差异倍数 Fold of differentiation
基因编号 Gene ID	基因注释 Gene annotation	-1 cm高光 -1 cm high light	-1 cm低光 -1 cm low light	+4 cm高光 +4 cm high light	+4 cm低光 +4 cm low light
GRMZM2G119865	EREB25	3.395	5.868	4.895	5.507
GRMZM2G416701	EREB81	-1.121	0.386	-2.032	0.006
GRMZM2G035502	DHAR2	-1.052	-0.609	-1.722	-0.590
GRMZM2G152777	GDSL-like Lipase super family	-6.103	-3.297	-4.868	-1.597
GRMZM2G036966	GeBP15	-4.058	-2.808	-6.100	-5.987
GRMZM2G102838	HCF136	-2.635	-1.989	-1.005	-0.101
GRMZM2G175782	NAC 036	-0.997	-2.026	-4.821	-2.077
GRMZM2G065105	novel plant snare 11	1.400	2.243	2.305	5.053
GRMZM2G077837	PEX11 family	-2.096	-1.119	-2.364	-0.932
GRMZM2G440605	SERPIN family	-4.433	-3.536	-4.030	-1.765
GRMZM2G014055	TH7	-3.021	-4.203	-4.390	-3.148
GRMZM2G320298	α/β-Hydrolases super family	2.852	2.902	6.346	6.103
GRMZM5G848603	α/β-Hydrolases super family	-1.411	-0.193	-2.830	0.020
GRMZM2G019018	Unknown	-1.615	-2.612	-2.580	-2.172
GRMZM2G038570	Unknown	2.631	2.820	3.442	4.732
GRMZM2G100568	Unknown	-2.655	-4.581	-4.803	-2.601
GRMZM2G103108	Unknown	4.154	5.141	4.077	5.266
GRMZM2G126900	Unknown	3.570	2.071	6.321	4.916
GRMZM2G130173	Unknown	-3.533	-1.656	-1.454	-0.961
GRMZM2G134020	Unknown	-3.388	-3.906	-4.870	-1.959
GRMZM2G140998	Unknown	-0.885	-0.505	-1.155	-0.425
GRMZM2G152808	Unknown	-0.755	-0.500	-2.393	-0.318
GRMZM2G156422	Unknown	-2.033	-0.869	-3.605	-0.505
GRMZM2G161761	Unknown	-4.532	-4.261	-5.043	-1.752
GRMZM2G307258	Unknown	2.870	4.177	5.357	6.317
GRMZM2G338406	Unknown	3.774	8.907	4.928	6.966
GRMZM2G430807	Unknown	-2.359	-2.589	-3.808	-1.547
GRMZM2G473480	Unknown	-2.406	0.586	-3.956	-0.926
GRMZM5G844094	Unknown	-3.144	-12.202	-2.588	-2.947
GRMZM5G879789	Unknown	-3.293	-1.208	-2.215	-1.200

Table 2

Table 3

Fig. 3

Table 4

Expression changes of genes related to phtosynthesis"

基因编号 Gene ID	基因注释 Gene annotation	差异倍数Fold of differentiation
基因编号 Gene ID	基因注释 Gene annotation	-1 cm高光 -1 cm high light	-1 cm低光 -1 cm low light	+4 cm高光 +4 cm high light	+4 cm低光 +4 cm low light
GRMZM2G027955	APL2	0.363	0.014	-3.367	-0.712
GRMZM2G038335	ATL43	0.466	1.610	6.382	3.383
GRMZM2G055973	ATL5D	0.202	1.122	1.990	2.388
GRMZM5G803981	BAM6	-0.701	0.242	7.007	5.671
GRMZM2G035749	BMY8	2.264	3.059	3.913	2.411
GRMZM2G144782	CHYR1	1.399	1.730	3.491	3.889
GRMZM2G118737	CINV2	2.171	1.315	3.114	3.273
GRMZM2G170842	CINV2	1.900	1.495	4.975	3.865
GRMZM2G316136	CIPK1	0.177	-1.274	-3.614	-0.607
GRMZM2G412601	CIPK6	-2.724	1.050	-5.620	-0.632
GRMZM2G129399	Cu²⁺ transporter family	2.224	2.597	7.007	5.671
GRMZM2G092427	CP24	-1.268	1.812	1.223	0.921
GRMZM2G018798	DRIP2	1.574	1.353	3.985	4.302
GRMZM2G102838	HCF136	-2.635	-1.989	-1.005	-0.101
GRMZM2G107402	HEMA1	1.354	1.848	3.799	2.464
GRMZM2G092311	LHCA1	-0.452	1.233	0.342	1.112
GRMZM2G072280	LHCA2	-0.022	1.221	0.560	0.742
GRMZM2G160268	LHCA4	-0.821	1.270	0.318	1.076
GRMZM2G018627	LHCB2	-1.017	1.355	-0.778	1.679
GRMZM2G057281	LHCB3	-2.182	2.157	0.622	1.146
GRMZM2G033885	LHCB4	-1.523	1.234	0.073	1.189
GRMZM2G056270	MIEL1	0.884	0.851	1.419	2.535
GRMZM2G085019	NADP-ME	2.017	0.731	1.749	2.936
GRMZM2G463280	PRK	0.144	0.970	1.614	2.304
GRMZM2G016066	PSAE	-0.828	1.610	0.273	1.321
GRMZM2G358180	PSBC	1.273	-2.181	1.263	-0.430
GRMZM5G831399	PSBH	-0.391	-0.792	1.472	0.186
GRMZM2G113349	PSBO2	-0.358	1.042	1.063	1.448
GRMZM2G175562	PSBO2	-0.914	-0.302	-1.147	0.748
GRMZM5G870219	PsbQ-like 2	-0.061	0.929	0.768	1.382
GRMZM5G804323	QA	-0.181	0.960	-0.524	1.436
GRMZM2G440259	RING/U-box super family	0.362	1.663	4.823	2.293
GRMZM2G127147	RS6	1.815	2.503	4.575	3.283
GRMZM2G440605	SERPIN1	-4.433	-3.536	-4.030	-1.765
GRMZM2G399484	SET7/9 family	1.659	1.377	6.716	4.757
GRMZM2G037265	SIP1	2.568	1.687	5.697	4.360
基因编号 Gene ID	基因注释 Gene annotation	差异倍数Fold of differentiation
基因编号 Gene ID	基因注释 Gene annotation	-1 cm高光 -1 cm high light	-1 cm低光 -1 cm low light	+4 cm高光 +4 cm high light	+4 cm低光 +4 cm low light
GRMZM2G050177	SIP1	2.227	2.731	4.181	3.938
GRMZM2G050273	SIP1	1.597	1.929	4.194	4.096
GRMZM2G311756	SIP2	2.095	0.026	0.963	1.656
GRMZM2G010349	STN7	0.733	0.625	0.431	1.823
GRMZM2G701201	STN8	-0.394	1.410	0.638	1.336
GRMZM2G160460	sugar transporter family	2.639	0.955	4.748	3.755
GRMZM2G372297	TAP38	0.692	0.543	-0.052	1.396
GRMZM2G389118	TOC159	0.637	0.852	2.614	3.502
GRMZM2G123277	TPS7	1.995	0.794	3.352	3.394
GRMZM2G120674	UBC2	0.985	1.273	2.416	2.546

Table 4

Fig. 4

Table 5

Expression comparison of transcription factors between wild type and mutant (hcf136)"

基因编号 Gene ID	基因注释 Gene annotation	差异倍数Fold of differentiation
基因编号 Gene ID	基因注释 Gene annotation	-1 cm高光 -1 cm high light	-1 cm低光 -1 cm low light	+4 cm高光 +4 cm high light	+4 cm低光 +4 cm low light
GRMZM2G119865	EREB25	3.395	5.868	4.895	5.507
GRMZM2G416701	EREB81	-1.121	0.386	-2.032	0.006
GRMZM2G390641	ARF21	-2.515	1.222	-1.175	0.441
GRMZM2G030710	ARF24	0.523	0.488	-0.654	0.263
GRMZM2G145146	bHLH107	-1.942	1.531	0.450	2.132
GRMZM2G083504	bHLH121	-1.198	1.049	-1.339	0.186
GRMZM2G144275	bHLH136	-0.999	0.794	-1.633	0.319
GRMZM2G479885	bZIP39	-1.030	1.001	-1.996	0.247
GRMZM2G311665	C3H20	-1.447	1.247	-2.148	-0.910
GRMZM2G426154	EMB2219	1.397	0.681	0.784	0.919
GRMZM2G457562	EREB113	-0.211	0.335	8.063	5.344
GRMZM2G421033	EREB156	-1.105	3.042	2.755	3.012
GRMZM2G119865	EREB25	3.395	58.418	4.895	5.507
GRMZM2G031983	GATA21	-2.090	0.577	-2.982	-0.184
GRMZM2G036966	GBP15	-4.058	0.143	-6.100	-9.310
GRMZM2G036966	GeBP15	-4.058	-2.808	-6.100	-2.467
GRMZM2G026833	GLK1	-1.780	1.072	-1.934	0.114
GRMZM2G056600	HB120	-2.236	0.706	-3.776	-0.039
GRMZM2G137046	HY5	-0.400	1.143	2.085	2.266
GRMZM2G044902	LBD6	0.993	1.143	2.556	2.376
GRMZM2G078820	MYB158	-2.055	0.609	-0.700	2.263
GRMZM2G048136	MYB59	0.376	0.665	-2.822	-0.298
GRMZM2G049378	MYBR115	-1.092	-1.268	-4.646	-2.738
GRMZM2G175782	NAC 36	-1.034	-2.033	-4.821	-2.081
GRMZM2G114850	NAC1	1.014	3.187	2.749	2.706
GRMZM2G167018	NAC110	-0.046	1.303	-0.666	-0.054
基因编号 Gene ID	基因注释 Gene annotation	差异倍数Fold of differentiation
基因编号 Gene ID	基因注释 Gene annotation	-1 cm高光 -1 cm high light	-1 cm低光 -1 cm low light	+4 cm高光 +4 cm high light	+4 cm低光 +4 cm low light
GRMZM2G127379	NAC25	5.827	2.426	8.537	4.905
GRMZM5G898290	NAC40	-0.827	0.612	-1.000	-0.273
GRMZM2G162739	NAC5	0.588	1.387	4.264	3.045
GRMZM2G155370	Orphan28	-1.049	1.049	0.054	-1.533
GRMZM2G350711	Orphan307	0.301	1.242	2.251	3.453
GRMZM2G016756	PIL5	-1.352	1.159	-5.544	-0.196
GRMZM2G065374	PIL6	-0.267	0.539	-1.561	0.101
GRMZM2G023872	RGA1	6.869	1.363	9.580	7.073
GRMZM2G046816	TUB3	0.056	0.152	-2.410	0.116
GRMZM2G064775	ZIM29	8.189	4.106	10.844	3.384

Table 5

References 29

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材料 Material	胞间CO₂浓度 C_i	蒸腾速率 E	气孔导度 G_s	净光合速率 P_n	叶绿素a的含量 C_a(mg L^-1)	叶绿素b的含量 C_b(mg L^-1)
野生型WT	182.56±16.38	1.54±0.12	159±16.38	14.80±1.67	28.54±1.77	14.21±3.78
杂合体HET	175.72±20.25	1.46±0.10	161±14.63	14.57±1.42	27.92±2.53	12.35±4.62
突变体hcf136	373.61±36.57^*	0.67±0.22^*	62±25.21^*	-2.34±1.29^*	15.48±3.36^*	5.61±0.86^*

不同组分 Different component	高光 High light	低光 Low light	高光和低光 High light and low light
-1 cm上调 -1 cm up-regulated	2979	70	60
-1 cm下调 -1 cm down-regulated	2021	115	41
+4 cm上调 +4 cm up-regulated	2838	347	272
+4 cm下调 +4 cm down-regulated	2714	129	117

Transcriptome Analysis on a Maize Photosynthetic Mutant hcf136 (high chlorophyll fluorescence 136)

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