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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 1029-1041.doi: 10.3724/SP.J.1006.2012.01029

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Genetic Diversity of Agronomic Traits and Association Analysis with SRAP Markers in Flue-Cured Tobacco (Nicotiana tabacum) Varieties from Home and Abroad

ZHANG Ji-Shun,WANG Ren-Gang,YANG Chun-Yuan,WU Chun,SHI Yue-Wei,WANG Zhi-Hong,WANG Yi,REN Xue-Liang*   

  1. Guizhou Institute of Tobacco Sciences, Guiyang 550081, China
  • Received:2011-11-02 Revised:2012-02-22 Online:2012-06-12 Published:2012-03-29
  • Contact: 任学良, E-mail: renxuel@126.com, Tel: 0851-4117133

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Abstract: To assess the genetic diversity of flue-cured tobacco germplasm, developing the elite gene resources, we selected 258 flue-cured tobacco varieties from home and abroad to investigate 13 agronomic traits in two locations with different environments. The genetic diversity and population structure were analyzed with 597 SRAP markers produced by 16 primer combinations. Different association models were tested and the most effective model was chosen for the population to make the association analysis between agronomic traits and SRAP markers. The results revealed that abundant phenotypic and genetic diversity existed in the flue-cured tobacco germplasm accessions. Flue-cured tobaccos from China showed less diversity than those from other countries. The accessions could be divided into seven subpopulations and the classification result was significantly correlation with geographic origins and hereditary relationship. Population structure within exotic varieties was multiplex. MIM_Q+K and MIM_PCA+K were effective models for association analysis between agronomic traits and SRAP markers. A total of 18 markers were found to be significantly associated (–log P>2, P<0.01) with six agronomic traits. Among them, markers associated with plant height (me1/em9-16, me1/em9-36, me4/em9-1), leaf number (me1/em2-14, me7/em5-34), bottom leaf width (me6/em2-6) and lumbar leaf length (me2/em6-15) were stably detected in different environments. This study provides evidence for identification of elite flue-cured tobacco germplasm, prediction of candidate gene and marker-assisted selection.

Key words: Flue-cured tobacco (Nicotiana tabacum), Genetic diversity, Agronomic traits, Association analysis, SRAP marker

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