Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1583-1591.doi: 10.3724/SP.J.1006.2012.01583

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identifications of DNA Sequences Encoding Key Region of SCR Interacting with SRK Extracellular Domain by Using Yeast Two-Hybrid System

XUE Li-Yan1,**,LUO Bing1,3,**,ZHU Li-Quan1,*,YANG Yong-Jun1,ZHANG He-Cui1,CHANG Deng-Long1,CHEN Song1,PENG Yi-Bo1,YANG Hong1,ZENG Jing1,YANG Kun1,GAO Qi-Guo2,LI Cheng-Qiong2,REN Xue-Song2,WANG Xiao-Jia2   

  1. 1Plant Physiology and Biochemistry Laboratory of Southwest University, Chongqing 400716, China; 2 Chongqing Key Laboratory of Olericulture, Chongqing 400716, China; 3 College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Received:2011-12-16 Revised:2012-04-20 Online:2012-09-12 Published:2012-07-03
  • Contact: 朱利泉, E-mail: zhuliquan@swu.edu.cn, Tel: 023-68250794

PDF

1223

Abstract: The S-locus cystein-rich protein (SCR) is the male-determining factor of self-incompatibility in Brassica. In this study, the SCR fragments with different lengths amplified from Brassica oleracea L. were ligated with pGBKT7 to construct recombinant bait plasmids, which were then transformed into yeast Y2HGold cells for detecting their interaction with the S-locus receptor kinase extracellular domain (eSRK) in Brassica oleracea by using the yeast two-hybrid system. The results showed that recombinant vectors were not activated autonomously. The full length SCR could interact with eSRK, and the core region in the SCR was located between 97 and 186 bp. Moreover, the result also indicated that the splicing site of signal peptides of this haplotype SCR and its several adjacent amino acid residues could affect the interaction. These conclusions add some novel insights into the mechanism research of self- incompatibility in Brassica.

Key words: Brassica oleracea, Self-incompatibility, SCR, Yeast two-hybrid system

[1]Hiscock S J. Pollen recognition during the self-incompatibility response in plants. Genome Biol, 2002, 3(2): reviews 1004.1–1004.6

[2]Lu Y(芦岩), Li Y-H(李玉花). Signal transduction of self-incompatibility in Brassica. Plant Physiol Commun (植物生理学通讯), 2005, 41(4): 547–552 (in Chinese with English abstract)

[3]Stone S L, Anderson E M, Mullen R T, Goring D R. ARC1 is an E3 Ubiquitin ligase and promotes the Ubiquitination of proteins during the rejection of self-incompatible Brassica pollen. Plant Cell, 2003, 15: 885–898

[4]Kakita M,, Shimosato H, Murasea K, Isogai A, Takayama S. Direct interaction between S-locus receptor kinase and M-locus protein kinase involved in Brassica self-incompatibility signaling. Plant Biotechnol, 2007, 24: 185–190

[5]Schopfer C R, Nasrallahm M E, Nasrallah J B. The male determinant of self-incompatibility in Brassica. Science, 1999, 286: 1697–1700

[6]Watanabe M, Ito A, Takada Y, Ninomiya C, Kakizaki T, Takahata Y, Hatakeyama K, Hinata K, Suzuki G, Takasaki T, Satta Y, Shiba H, Takayama S, Isogai A. Highly divergent sequences of the pollen self-incompatibility (S) gene in class-I S haplotypes of Brassica campestris (syn. rapa) L. FEBS Lett, 2000, 473: 139–144

[7]Cui H-Y(崔海洋), Lai Z(赖钊), Xue Y-B(薛勇彪). A rewarding fifteen years: from SLG to SCR/ SP11. Acta Bot Sin (植物学报), 2001, 43(1): 1–5 (in Chinese with English abstract)

[8]Lan X-G(蓝兴国), Yang J(杨佳), Li Y-H(李玉花). Isolation and functional analysis of a S13-b-locus cysteine-rich gene of ornamental kale (Brassica oleracea var. acephala ). Acta Hort Sin (园艺学报), 2009, 36 (4): 539–544 (in Chinese with English abstract)

[9]Takayama S, Shimosato H, Shiba H, Funato M, Che F S, Watanabe M, Iwano M, Isogai A. Direct ligand-receptor complex interaction controls Brassica self-incompatibility. Nature, 2001, 413: 534–538

[10]Mishima M, Takayama S, Sasaki K I, Jee J G, Kojima C, Isogai A, Shirakawa M. Structure of the male determinant factor for Brassica self-incompatibility. J Biol Chem, 2003, 278: 36389–36395

[11]Luo B(罗兵), Xue L-Y(薛丽琰), Zhu L-Q(朱利泉), Zhang H-C(张贺翠), Peng Y-B(彭一波), Chen S(陈松), Yang H(杨红), Yang K(杨昆), Li C-Q(李成琼), Wang X-J(王小佳). Detection of interactions between SCR and SRK in Brassica oleracea L. by yeast two-hybrid system. Acta Agron Sin (作物学报), 2011, 37(4): 579–586 (in Chinese with English abstract)

[12]Yang H(杨红), Zhu L-Q(朱利泉), Zhang H-C(张贺翠), Yang Y-J(杨永军), Xue L-Y(薛丽琰), Yang K(杨昆), Yu H(余浩), Peng Y-B(彭一波), Luo B(罗兵), Wu Z-G(吴志刚), Huang D(黄丹), Gao Q-G(高启国), Wang X-J(王小佳). Study on the interactions between the truncated fragments of SCR and eSRK from Brassica oleracea L. by a yeast two-hybrid system. Sci Agric Sin (中国农业科学), 2011, 44(9): 1953–1962 (in Chinese with English abstract)

[13]Peng Y-B(彭一波), Zhu L-Q(朱利泉), Yang H(杨红), Xue L-Y(薛丽琰), Luo B(罗兵), Wu Z-G(吴志刚), Huang D(黄丹), Yang K(杨昆), Gao Q-G(高启国), Li C-Q(李成琼), Ren X-S(任雪松), Wang X-J(王小佳). Studies on the construction of S domain of SRK and SCR gene’s yeast expression vectors and their interaction in yeast two-hybrid system. Acta Hort Sin (园艺学报), 2011, 38(8): 1479–1488 (in Chinese with English abstract)

[14]Zheng B(郑斌), Zhan X-M(詹希美). Signal peptide sequence and its application to protein expression. Lett Biotechnol (生物技术通讯), 2005, 3(16): 296–298 (in Chinese with English abstract)

[15]Sato Y, Fujimoto R, Toriyama K, Nishio T. Commonality of self-recognition specificity of S haplotypes between Brassica oleracea and Brassica rapa. Plant Mol Biol, 2003, 52: 617– 626

[16]Hiscock S J. and McInnis S M. Pollen recognition and rejection during the sporophytic self-incompatibility response: Brassica and beyond. Trends Plant Sci, 2003, 12: 606–613

[17]Kitashiba H, Liu P, Nishio T, Nasrallah J B, Nasrallah M E. Functional test of Brassica self-incompatibility modifiers in Arabidopsis thaliana. Proc Natl Acad Sci USA, 2011, 108: 18173–18178

[18]Kachroo A, Schopfer C R, Nasrallah M E, Nasrallah J B. Allele-specific receptor–ligand interactions in Brassica self-incompatibility. Science, 2001, 293: 1824–1826
[1] CUI Lian-Hua, ZHAN Wei-Min, YANG Lu-Hao, WANG Shao-Ci, MA Wen-Qi, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping, YANG Qing-Hua. Molecular cloning of two maize (Zea mays) ZmCOP1 genes and their transcription abundances in response to different light treatments [J]. Acta Agronomica Sinica, 2022, 48(6): 1312-1324.
[2] QIN Lu, HAN Pei-Pei, CHANG Hai-Bin, GU Chi-Ming, HUANG Wei, LI Yin-Shui, LIAO Xiang-Sheng, XIE Li-Hua, LIAO Xing. Screening of rapeseed germplasms with low nitrogen tolerance and the evaluation of its potential application as green manure [J]. Acta Agronomica Sinica, 2022, 48(6): 1488-1501.
[3] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[4] ZHANG Yi-Zhong, ZENG Wen-Yi, DENG Lin-Qiong, ZHANG He-Cui, LIU Qian-Ying, ZUO Tong-Hong, XIE Qin-Qin, HU Deng-Ke, YUAN Chong-Mo, LIAN Xiao-Ping, ZHU Li-Quan. Codon usage bias analysis of S-locus genes SRK, SLG, and SP11/SCR in Brassica oleracea [J]. Acta Agronomica Sinica, 2022, 48(5): 1152-1168.
[5] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
[6] XIE Qin-Qin, ZUO Tong-Hong, HU Deng-Ke, LIU Qian-Ying, ZHANG Yi-Zhong, ZHANG He-Cui, ZENG Wen-Yi, YUAN Chong-Mo, ZHU Li-Quan. Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea [J]. Acta Agronomica Sinica, 2022, 48(1): 108-120.
[7] WEI Yi-Hao, YU Mei-Qin, ZHANG Xiao-Jiao, WANG Lu-Lu, ZHANG Zhi-Yong, MA Xin-Ming, LI Hui-Qing, WANG Xiao-Chun. Alternative splicing analysis of wheat glutamine synthase genes [J]. Acta Agronomica Sinica, 2022, 48(1): 40-47.
[8] LI Ling-Hong, ZHANG Zhe, CHEN Yong-Ming, YOU Ming-Shan, NI Zhong-Fu, XING Jie-Wen. Transcriptome profiling of glossy1 mutant with glossy glume in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2022, 48(1): 48-62.
[9] WANG Ying, GAO Fang, LIU Zhao-Xin, ZHAO Ji-Hao, LAI Hua-Jiang, PAN Xiao-Yi, BI Chen, LI Xiang-Dong, YANG Dong-Qing. Identification of gene co-expression modules of peanut main stem growth by WGCNA [J]. Acta Agronomica Sinica, 2021, 47(9): 1639-1653.
[10] ZHANG He, JIANG Chun-Ji, YIN Dong-Mei, DONG Jia-Le, REN Jing-Yao, ZHAO Xin-Hua, ZHONG Chao, WANG Xiao-Guang, YU Hai-Qiu. Establishment of comprehensive evaluation system for cold tolerance and screening of cold-tolerance germplasm in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1753-1767.
[11] CAO Liang, DU Xin, YU Gao-Bo, JIN Xi-Jun, ZHANG Ming-Cong, REN Chun-Yuan, WANG Meng-Xue, ZHANG Yu-Xian. Regulation of carbon and nitrogen metabolism in leaf of soybean cultivar Suinong 26 at seed-filling stage under drought stress by exogenous melatonin [J]. Acta Agronomica Sinica, 2021, 47(9): 1779-1790.
[12] YIN Ming, YANG Da-Wei, TANG Hui-Juan, PAN Gen, LI De-Fang, ZHAO Li-Ning, HUANG Si-Qi. Genome-wide identification of GRAS transcription factor and expression analysis in response to cadmium stresses in hemp (Cannabis sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(6): 1054-1069.
[13] HUANG Wen-Gong, JIANG Wei-Dong, YAO Yu-Bo, SONG Xi-Xia, LIU Yan, CHEN Si, ZHAO Dong-Sheng, WU Guang-Wen, YUAN Hong-Mei, REN Chuan-Ying, SUN Zhong-Yi, WU Jian-Zhong, KANG Qing-Hua. Transcriptome profiling of flax (Linum usttatissimum L.) response to low potassium stress [J]. Acta Agronomica Sinica, 2021, 47(6): 1070-1081.
[14] GE Min, WANG Yuan-Cong, NING Li-Hua, HU Meng-Mei, SHI Xi, ZHAO Han. Function analysis of nitrogen-responsive transcription factor ZmNLP5 affecting root growth in maize [J]. Acta Agronomica Sinica, 2021, 47(5): 807-813.
[15] MA Gui-Fang, MAN Xia-Xia, ZHANG Yi-Juan, GAO Hao, SUN Zhao-Xia, LI Hong-Ying, HAN Yuan-Huai, HOU Si-Yu. Integrated analysis between folate metabolites profiles and transcriptome of panicle in foxtail millet [J]. Acta Agronomica Sinica, 2021, 47(5): 837-846.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd