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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1583-1591.doi: 10.3724/SP.J.1006.2012.01583

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identifications of DNA Sequences Encoding Key Region of SCR Interacting with SRK Extracellular Domain by Using Yeast Two-Hybrid System

XUE Li-Yan1,**,LUO Bing1,3,**,ZHU Li-Quan1,*,YANG Yong-Jun1,ZHANG He-Cui1,CHANG Deng-Long1,CHEN Song1,PENG Yi-Bo1,YANG Hong1,ZENG Jing1,YANG Kun1,GAO Qi-Guo2,LI Cheng-Qiong2,REN Xue-Song2,WANG Xiao-Jia2   

  1. 1Plant Physiology and Biochemistry Laboratory of Southwest University, Chongqing 400716, China; 2 Chongqing Key Laboratory of Olericulture, Chongqing 400716, China; 3 College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Received:2011-12-16 Revised:2012-04-20 Online:2012-09-12 Published:2012-07-03
  • Contact: 朱利泉, E-mail: zhuliquan@swu.edu.cn, Tel: 023-68250794

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Abstract: The S-locus cystein-rich protein (SCR) is the male-determining factor of self-incompatibility in Brassica. In this study, the SCR fragments with different lengths amplified from Brassica oleracea L. were ligated with pGBKT7 to construct recombinant bait plasmids, which were then transformed into yeast Y2HGold cells for detecting their interaction with the S-locus receptor kinase extracellular domain (eSRK) in Brassica oleracea by using the yeast two-hybrid system. The results showed that recombinant vectors were not activated autonomously. The full length SCR could interact with eSRK, and the core region in the SCR was located between 97 and 186 bp. Moreover, the result also indicated that the splicing site of signal peptides of this haplotype SCR and its several adjacent amino acid residues could affect the interaction. These conclusions add some novel insights into the mechanism research of self- incompatibility in Brassica.

Key words: Brassica oleracea, Self-incompatibility, SCR, Yeast two-hybrid system

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