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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1631-1639.doi: 10.3724/SP.J.1006.2012.01631

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of eSRK Chimeras and Interaction between eSRK Chimeras and SCRs from Brassica oleracea L.

WEI Jing-Yi,GAO Qi-Guo*,REN Xue-Song,WANG Xiao-Jia*,LI Cheng-Qiong,SONG Ming   

  1. College of Horticulture and Landscape Architecture, Southwest University / Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China
  • Received:2012-01-19 Revised:2012-04-20 Online:2012-09-12 Published:2012-07-03
  • Contact: 高启国, E-mail: gaoqg2004031@163.com; 王小佳, E-mail: wxj@swu.edu.cn

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Abstract: Self-incompatibility in Brassica is mediated by allele-specific interactions between stigma-expressed S-locus receptor kinase (SRK) and pollen coat-localized S-locus cysteinerich (SCR) ligands encoded by the S-locus haplotype. To identify amino acid fragments within the SRK extracellular domain (eSRK) that are required for ligand-selective activation, we constructed chimeric eSRK between two S-locus haplotypes in Brassica oleracea, and identified the interaction between eSRK chimeras and SCRs by yeast two-hybrid system. The results showed that SRKE (not chimera) could interact with SCRE, and SRKF could interact with SCRF. All of eSRK chimeras could not interact with SCRs. The hypervariable regions, HVI and HVII, were essential for specificity in the SRK-SCR interaction. However, eSRK chimeras could not interact with SCRF, although they contained hypervariable regions come from eSRKF, which should be related with the overall sequence or 3D conformation of the segments determining SI specificity.

Key words: Self-incompatibility, SRK, SCR, Yeast two-hybrid system

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