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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1640-1648.doi: 10.3724/SP.J.1006.2012.01640

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Analysis of Introgression Lines on Chromosomes 1H–7H in Barley

LAI Yong1,2,FENG Jing-Xia1,2,SI Er-Jing1,2,LI Bao-Chun3,MENG Ya-Xiong1,2,MA Xiao-Le2,YANG Ke1,2,SHANG Xu-Wu2,WANG Hua-Jun1,2,*   

  1. 1 Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement / Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China; 2 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 3 College of Life Sciences and Technology of Gansu Agricultural University, Lanzhou 730070, China
  • Received:2012-01-15 Revised:2012-05-13 Online:2012-09-12 Published:2012-07-03
  • Contact: 王化俊, E-mail: whuajun@yahoo.com

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Abstract: To provide a suitable population for quantitative trait locus (QTL) mapping and genetic research of favorable genes in barley (Hordeum valgare L.), we constructed a set of introgression lines (ILs, BC3F2) with the genetic background of recurrent parent Scarlett using 96 SSR markers that cover the whole genome of wild barley ISR42-8. This IL population contained 66 lines, and the target segments were determined with the 96 SSR markers distributed on chromosomes 1H to 7H of barley. The average length of target segments was 27.6 cM. The line IL-52 carried the longest introgressed segment (100.5 cM), and the line IL-50 carried the shortest introgressed segment (1.5 cM). There were 33 lines containing a single segment. The clustering analysis showed that the genetic backgrounds of these introgression lines were rather similar with each other. The genetic similarity coefficients ranged from 0.708 to 1.000, with the mean of 0.917. A QTL for tiller number was mapped in the interval of 87.5–110.0 cM on chromosome 4H, the size of which was 22.5 cM, and the polymorphism marker was MGB396.

Key words: Barley, Introgression line, Molecular mark, QTL mapping

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