Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (5): 1279-1287.doi: 10.3724/SP.J.1006.2022.11048

• RESEARCH NOTES • Previous Articles     Next Articles

Drought resistance identification and drought resistance indexes screening of Tibetan hulless barley resources at adult stage

WANG Xing-Rong1(), LI Yue1, ZHANG Yan-Jun1, LI Yong-Sheng1, WANG Jun-Cheng3, XU Yin-Ping2, QI Xu-Sheng1,*()   

  1. 1Institute of Crop Sciences, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
    2Institute of Industrial Crops and Malting Barley, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
    3College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu, China
  • Received:2021-05-10 Accepted:2021-09-10 Online:2022-05-12 Published:2021-10-15
  • Contact: QI Xu-Sheng E-mail:wxr_0618@163.com;qixusheng6608@sina.com
  • Supported by:
    Agricultural Science and Technology Innovation Project of Gansu Academy of Agricultural Sciences(2019GAAS08);Central Government Guides Local Science and Technology Development Projects;Science and Technology Planning Project of Gansu Province(18JR2TA020)

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Abstract:

Drought is one of the main factors affecting the production of hulless barley in the Qinghai-Tibet Plateau. Identification of drought resistance of hulless barley germplasm resources at adult stage and screening of reliable drought resistance indexes can provide basis for breeding drought resistant varieties. In the present study, a total of 238 hulless barley germplasms were used as materials, and two treatments of normal irrigation and drought stress in the field conditions were conducted in Zhangye of Gansu Province in 2019 and 2020, respectively. Seven indexes, such as the plant height, spike number per plant, biomass per plant, grain weight per spike, spike length, 1000-grain weight, and yield were determined. The drought resistance comprehensive evaluation value (D-value), comprehensive drought resistance coefficient (CDC-value), and weight drought resistance coefficient (WDC-value) were used to identify drought resistance at adult stage of hulless barley germplasms. The results showed that the effects of drought stress on all indexes were extremely significant, and the correlation analysis showed that the yield was significantly positively correlated with plant height and 1000-grain weight at P < 0.01, and significantly positively correlated with the grain weight per panicle at P<0.05. Frequency analysis showed that the sensitivity of seven indexes to drought stress was plant height, yield, biomass per plant, grain weight per ear, number of ears per plant, ear length and 1000-grain weight, respectively. The ranks of drought resistance of germplasms based on the D-value, CDC-value, and WDC-value were similar. Grey correlation degree analysis showed that the correlation degree between DC-value and D-value was in the order of yield, biomass per plant, grain weight per spike, plant height, number of spikelets per plant, ear length and 1000-grain weight, which was basically consistent with the close degree between DC-value and WDC-value of each index. Stepwise regression analysis showed that yield, plant height, panicle number per plant, 1000-grain weight and biomass per plant could be used as reliable indexes for drought resistance identification of hulless barley in adult stage. D-value was determined as the appropriate method for drought resistance identification. Based on D-value, the tested hulless barley germplasms were divided into five drought resistance grades, and 38 germplasms were classified in grade I, such as Ziyanwoqingke, Liulengqingke, Ziqingke, ZDM8125, Tuhuluqingke, etc. These results provided important basic materials and theoretical reference for the breeding of drought-resistant varieties and the study of drought-resistant mechanism of hulless barley.

Key words: hulless barley, adult stage, drought resistance, drought resistance indexes

Table S1

Origin and classification of drought resistance grades of tested hulless barley germplasm"

编号
Number		 种质名称
Name		 来源
Origin		 D值
D-value		 分级
Grade		 CDC值
CDC-value		 分级
Grade		 WDC值
WDC-value		 分级
Grade
1 长芒青稞 Changmangqingke 甘肃临潭 Lintan, Gansu 0.424 4 0.662 3 0.648 3
2 麻青稞 Maqingke 甘肃临潭Lintan, Gansu 0.370 5 0.596 5 0.586 5
3 碌曲白青稞 Luqubaiqingke 甘肃碌曲 Luqu, Gansu 0.605 1 0.732 2 0.726 3
4 碌曲紫青稞 Luquziqingke 甘肃碌曲Luqu, Gansu 0.585 1 0.788 1 0.802 1
5 土房紫青稞 Tufangziqingke 甘肃夏河Xiahe, Gansu 0.591 1 0.842 1 0.860 1
6 迭部紫青稞 Diebuziqingke 甘肃迭部Diebu, Gansu 0.471 3 0.678 3 0.678 3
7 舟曲春青稞 Zhouquchunqingke 甘肃舟曲Zhouqu, Gansu 0.340 5 0.579 5 0.569 5
8 宕昌青稞(蓝) Tanchangqingke (lan) 甘肃宕昌Tanchang, Gansu 0.497 3 0.655 3 0.636 3
9 宕昌青稞(黄) Tanchangqingke (huang) 甘肃宕昌Tanchang, Gansu 0.544 2 0.749 2 0.751 2
10 庄浪青稞 Zhuanglangqingke 甘肃庄浪Zhuanglang, Gansu 0.363 5 0.578 5 0.569 5
11 张掖春青稞 Zhangyechunqingke 甘肃张掖Zhangye, Gansu 0.382 5 0.631 4 0.635 3
12 榆中青稞(黄) Yuzhongqingke (huang) 甘肃榆中Yuzhong, Gansu 0.423 4 0.587 5 0.566 5
13 牡丹青稞 Mudanqingke 甘肃临夏Linxia, Gansu 0.387 5 0.603 4 0.604 4
14 榆中青稞(蓝) Yuzhongqingke (lan) 甘肃榆中Yuzhong, Gansu 0.556 1 0.708 3 0.689 3
15 岷县青稞 Minxianqingke 甘肃岷县Minxian, Gansu 0.626 1 0.757 2 0.735 2
16 漳县青稞 Zhangxianqingke 甘肃漳县Zhangxian, Gansu 0.498 3 0.746 2 0.760 2
17 漳县长青稞 Zhangxianchangqingke 甘肃漳县Zhangxian, Gansu 0.485 3 0.750 2 0.765 2
18 漳县长芒青稞 Zhangxianchangmangqingke 甘肃漳县Zhangxian, Gansu 0.397 4 0.611 4 0.605 4
19 金塔有芒青稞 Jintayoumangqingke 甘肃金塔Jinta, Gansu 0.426 4 0.661 3 0.661 3
20 古浪青稞 Gulangqingke 甘肃古浪Gulang, Gansu 0.596 1 0.797 1 0.806 1
21 会宁矮秆青稞 Huiningaiganqingke 甘肃会宁Huining, Gansu 0.459 3 0.688 3 0.697 3
22 欧娃青稞 Ouwaqingke 甘肃岷县Minxian, Gansu 0.426 4 0.646 3 0.658 3
23 连皮青稞 Lianpiqingke 甘肃临泽Linze, Gansu 0.483 3 0.661 3 0.649 3
24 二棱青稞1 Erlengqingke 1 甘肃临夏Linxia, Gansu 0.568 1 0.780 1 0.781 1
25 二棱青稞2 Erlengqingke 2 甘肃临夏Linxia, Gansu 0.544 2 0.722 3 0.712 3
26 二棱紫青稞 Erlengziqingke 甘肃金塔Jinta, Gansu 0.524 2 0.774 1 0.794 1
27 青稞 Qingke 甘肃夏河Xiahe, Gansu 0.499 3 0.763 1 0.775 1
28 和尚青稞 Heshangqingke 甘肃积石山Jishishan, Gansu 0.542 2 0.763 1 0.744 2
29 紫青稞 Ziqingke 甘肃临夏Linxia, Gansu 0.447 3 0.630 4 0.620 4
30 紫青稞 Ziqingke 甘肃夏河Xiahe, Gansu 0.489 3 0.639 3 0.624 4
31 二混水青稞 Erhunshuiqingke 甘肃临泽Linze, Gansu 0.389 5 0.649 3 0.664 3
32 土葫芦紫青稞 Tuhuluziqingke 甘肃永昌Yongchang, Gansu 0.641 1 0.846 1 0.851 1
33 大青稞 Daqingke 甘肃临夏Linxia, Gansu 0.363 5 0.608 4 0.606 4
34 大麻青稞 Damaqingke 甘肃临洮Linao, Gansu 0.433 3 0.701 3 0.718 3
35 小青稞 Xiaoqingke 甘肃临夏Linxia, Gansu 0.482 3 0.694 3 0.710 3
36 无叶耳青稞 Wuyeerqingke 甘肃甘南Gannan, Gansu 0.574 1 0.741 2 0.733 2
37 无叶耳紫青稞 Wuyeerziqingke 甘肃夏河Xiahe, Gansu 0.421 4 0.599 5 0.580 5
38 无皮青稞 Wupiqingke 甘肃礼县Lixian, Gansu 0.342 5 0.546 5 0.539 5
39 无芒青稞 Wumangqingke 甘肃平凉Pingliang, Gansu 0.500 3 0.643 3 0.608 4
40 瓦兰青稞 Walanqingle 甘肃天祝Tianzhu, Gansu 0.455 3 0.634 4 0.623 4
41 长芒青稞 Changmangqingke 甘肃漳县Zhangxian, Gansu 0.331 5 0.600 4 0.611 4
42 长身子青稞 Changshenziqingke 甘肃临潭Lintan, Gansu 0.308 5 0.618 4 0.628 4
43 长青稞 Changqingke 甘肃临潭Lintan, Gansu 0.349 5 0.554 5 0.551 5
44 六棱子长芒白青稞 Liulengzichangmangbaiqingke 甘肃永昌Yongchang, Gansu 0.426 3 0.700 3 0.711 3
45 六棱小青稞1 Liulengxiaoqingke 1 甘肃临泽Linze, Gansu 0.426 4 0.565 5 0.543 5
46 六棱小青稞2 Liulengxiaoqingke 2 甘肃临泽Linze, Gansu 0.508 3 0.748 2 0.764 2
47 六棱子青稞 Liulengziqingke 甘肃临潭Lintan, Gansu 0.559 1 0.746 2 0.754 2
48 六棱头白青稞 Liulengtoubaiqingke 甘肃古浪Gulang, Gansu 0.406 4 0.685 3 0.696 3
49 六棱头青稞 Liulengtouqingke 甘肃金塔Jinta, Gansu 0.343 5 0.586 5 0.587 5
50 六棱大青稞 Liulengdaqingke 甘肃金塔Jinta, Gansu 0.321 5 0.523 5 0.521 5
51 六棱头紫青稞 Liulengtouziqingke 甘肃民乐Minle, Gansu 0.386 5 0.618 4 0.617 4
52 六棱青稞 Liulengqingke 甘肃敦煌Dunhuang, Gansu 0.314 5 0.572 5 0.570 5
53 六棱青稞 Liulengqingke 甘肃民勤Minqin, Gansu 0.443 3 0.668 3 0.670 3
54 六棱青稞 Liulengqingke 甘肃西和Xihe, Gansu 0.387 5 0.574 5 0.567 5
55 六棱青稞 Liulengqingke 甘肃漳县Zhangxian, Gansu 0.375 5 0.674 3 0.684 3
56 六棱青稞 Liulengqingke 甘肃舟曲Zhouqu, Gansu 0.457 3 0.635 4 0.627 4
57 六棱青稞 Liulengqingke 甘肃卓尼Zhuoni, Gansu 0.675 1 0.910 1 0.919 1
58 六棱紫青稞 Liulengziqingke 甘肃甘南Gannan, Gansu 0.333 5 0.560 5 0.564 5
59 六棱黑青稞 Liulengheiqingke 甘肃武威Wuwei, Gansu 0.616 1 0.768 1 0.750 2
60 六棱兰青稞 Liulenglanqingke 甘肃民乐Minle, Gansu 0.425 4 0.638 4 0.628 4
61 双腰青稞 Shuangyaoqingke 甘肃岷县Minxian, Gansu 0.479 3 0.694 3 0.695 3
62 本地麻青稞 Bendimaqingke 甘肃渭源Weiyuan, Gansu 0.351 5 0.610 4 0.609 4
63 四棱青稞 Silengqingke 甘肃民勤Minqin, Gansu 0.551 1 0.709 3 0.695 3
64 白皮青稞 Baipiqingke 甘肃张掖Zhangye, Gansu 0.581 1 0.758 2 0.767 1
65 白杈青稞 Baichaqingke 甘肃临夏Linxia, Gansu 0.476 3 0.693 3 0.683 3
66 白青稞 Baiqingke 甘肃岷县Minxian, Gansu 0.506 3 0.740 2 0.761 2
67 白青稞 Baiqingke 甘肃夏河Xiahe, Gansu 0.592 1 0.724 3 0.717 3
68 白青稞 Baiqingke 甘肃酒泉Jiuquan, Gansu 0.531 2 0.757 2 0.760 2
69 白青稞 Baiqingke 甘肃玉门Yumen, Gansu 0.448 3 0.644 3 0.634 3
70 白青稞 Baiqingke 甘肃敦煌Dunhuang, Gansu 0.411 4 0.622 4 0.617 4
71 白青稞1 Baiqingke 1 甘肃民乐Minle, Gansu 0.493 3 0.661 3 0.649 3
72 白青稞2 Baiqingke 2 甘肃民乐Minle, Gansu 0.382 5 0.589 5 0.587 5
73 白青稞3 Baiqingke 3 甘肃民乐Minle, Gansu 0.370 5 0.553 5 0.539 5
74 白青稞 Baiqingke 甘肃肃南Sunan, Gansu 0.391 4 0.595 5 0.596 4
75 白青稞 Baiqingke 甘肃武威Wuwei, Gansu 0.459 3 0.696 3 0.702 3
76 白青稞1 Baiqingke 1 甘肃景泰Jingtai, Gansu 0.523 2 0.740 2 0.733 2
77 白青稞2 Baiqingke 2 甘肃景泰Jingtai, Gansu 0.552 1 0.807 1 0.822 1
78 白青稞 Baiqingke 甘肃古浪Gulang, Gansu 0.528 2 0.720 3 0.714 3
79 白籽青稞 Baiziqingke 甘肃临夏Linxia, Gansu 0.444 3 0.655 3 0.645 3
80 老白青稞 Laobaiqingke 甘肃武都Wudu, Gansu 0.317 5 0.575 5 0.609 4
81 老红青稞 Laohongqingke 甘肃武都Wudu, Gansu 0.522 2 0.713 3 0.705 3
82 老青稞1 Laoqingke 1 甘肃漳县Zhangxian, Gansu 0.506 3 0.778 1 0.801 1
83 老青稞2 Laoqingke 2 甘肃漳县Zhangxian, Gansu 0.540 2 0.666 3 0.643 3
84 老青稞 Laoqingke 甘肃甘谷Gangu, Gansu 0.530 2 0.788 1 0.819 1
85 老青稞 Laoqingke 甘肃永靖Yongjing, Gansu 0.469 3 0.735 2 0.756 2
86 老青稞 Laoqingke 甘肃西和Xihe, Gansu 0.601 1 0.852 1 0.852 1
87 红青稞 Hongqingke 甘肃和政Hezheng, Gansu 0.433 3 0.690 3 0.697 3
88 红青稞 Hongqingke 甘肃夏河Xiahe, Gansu 0.456 3 0.708 3 0.712 3
89 兴隆青稞 Xinglongqingke 甘肃榆中Yuzhong, Gansu 0.401 4 0.617 4 0.606 4
90 孙家庄白青稞 Sunjiazhuangbaiqingke 甘肃临潭Lintan, Gansu 0.518 2 0.733 2 0.728 3
91 杈青稞 Chaqingke 甘肃临夏Linxia, Gansu 0.376 5 0.562 5 0.550 5
92 牡丹青稞 Mudanqingke 甘肃临夏Linxia, Gansu 0.412 4 0.665 3 0.670 3
93 青皮青稞1 Qingpiqingke 1 甘肃酒泉Jiuquan, Gansu 0.511 3 0.706 3 0.703 3
94 青皮青稞2 Qingpiqingke 2 甘肃酒泉Jiuquan, Gansu 0.441 3 0.627 4 0.610 4
95 青皮青稞 Qingpiqingke 甘肃张掖Zhangye, Gansu 0.517 2 0.695 3 0.680 3
96 青青稞 Qingqingke 甘肃永登Yongdeng, Gansu 0.419 4 0.653 3 0.669 3
97 青稞 Qingke 甘肃榆中Yuzhong, Gansu 0.387 5 0.569 5 0.552 5
98 青稞1 Qingke 1 甘肃清水Qingshui, Gansu 0.392 4 0.663 3 0.666 3
99 青稞2 Qingke 2 甘肃清水Qingshui, Gansu 0.420 4 0.672 3 0.686 3
100 青稞3 Qingke 3 甘肃清水Qingshui, Gansu 0.324 5 0.485 5 0.455 5
101 青稞 Qingke 甘肃天水Tianshui, Gansu 0.423 4 0.588 5 0.561 5
102 青稞 Qingke 甘肃张家川Zhangjiachuan, Gansu 0.401 4 0.639 4 0.643 3
103 青稞 Qingke 甘肃静宁Jingning, Gansu 0.424 4 0.596 5 0.575 5
104 青稞 Qingke 甘肃永靖Yongjing, Gansu 0.448 3 0.677 3 0.681 3
105 青稞 Qingke 甘肃安西Anxi, Gansu 0.398 4 0.542 5 0.521 5
106 青稞 Qingke 甘肃武威Wuwei, Gansu 0.335 5 0.538 5 0.523 5
107 青稞 Qingke 甘肃景泰Jintai, Gansu 0.416 4 0.617 4 0.621 4
108 青稞 Qingke 甘肃武山Wushan, Gansu 0.448 3 0.653 3 0.662 3
109 青稞 Qingke 甘肃清水Qingshui, Gansu 0.410 4 0.661 3 0.668 3
110 青稞 Qingke 甘肃卓尼Zhuoni, Gansu 0.581 1 0.8