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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (2): 275-284.doi: 10.3724/SP.J.1006.2021.04105

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of resistance gene against phytophthora root rot in new soybean lines breeded in Henan province

ZHANG Xue-Cui1(), SUN Su-Li1(), LU Wei-Guo2, LI Hai-Chao2, JIA Yan-Yan1, DUAN Can-Xing1, ZHU Zhen-Dong1,*()   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2Institute of Economic Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
  • Received:2020-05-12 Accepted:2020-09-13 Online:2021-02-12 Published:2020-09-28
  • Contact: ZHU Zhen-Dong E-mail:553870151@qq.com;sunsuli@caas.cn;zhuzhendong@caas.cn
  • Supported by:
    National Key Research and Development Program of China(2016YFD0100201);Special Fund for Agro-scientific Research in the Public Interest(201303018);Program of Protection of Crop Germplasm Resources(2019NWB036-12);Scientific Innovation Program of Chinese Academy of Agricultural Sciences.

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Abstract:

Phytophthora root rot is one of the destructive diseases affecting soybean production, which is a great threat to soybean production. Planting resistant soybean cultivars is the most effective way to control this disease. Henan province was located in the hinterland area of the summer-sowing soybean production region of Huang-Huai in China, which had the potential threat region of phytophthora root rot. The objective of this study was to screen effective resistance cultivars for disease control and resistance breeding by phenotypic identification and molecular detection of resistance gene. Sixty-four new soybean lines bred in Henan were evaluated for their resistance responses to two Phytophthora sojae isolates PsJS2 and Ps41-1 using the hypocotyls inoculation technique. The result showed that 35 lines and 16 lines were resistance to Ps41-1 and PsJS2, respectively. Sixteen lines and 10 lines were intermediate to Ps41-1 and PsJS2, respectively. And there were 16 lines resistance to both Ps41-1and PsJS2, accounting for 25% of tested lines. Sixty-four lines was detected for Phytophthora resistance gene by using molecular marker WZInDel11 co-segregating with a resistance gene RpsZheng. The results showed that, 13 of 16 lines resistant to both PsJS2 and Ps41-1 contain target band of WZInDel11, while 5 lines resistant to one of two P. sojae isolates show segregating to P. sojae produced heterozygous bands. The homozygous resistant plants of these lines segregating for resistance could be accurately detected by marker WZInDel11, and further were directly developed into homozygous resistant lines. Combining the results of pedigree analysis, it was speculated that two lines might contain the resistance gene RpsZheng, two lines might contain RpsYD29, and 14 lines might contain RpsZheng or its allele. In conclusion, the results indicated that the new soybean lines cultivated in Henan Province had excellent resistance sources to P. sojae. This study provides important information for disease control and resistance breeding.

Key words: Glycine max, phytophthora root rot, lines, resistance, molecular marker

Table 1

Phenotypic reaction of new soybean lines against two Phytophthora sojae isolates and their results of detection using marker WZInDel11 co-segregated with Rpszheng"

Fig. 1

Percentage of soybean lines resistance to two isolates of Phytophthora sojae, Ps41-1 and PsJS2 R: resistant; I: intermediate; S: susceptible."

Fig. 2

Detection of some soybean lines using marker WZInDel11 Williams and Zheng 97196 are the controls of susceptible and resistant varieties, respectively. Luodou 16106, Zhengdou 1304, Luodou 16095, Zhoudou 47, and Zhengdou 1526 are all the resistant lines. Zhoudou 39, Luodou 1420, Shangdou 191, Pudou 103, and Wanhuang 5 are all the susceptible lines. Zheng 15234, Luo 8825, Luodou 8816, Zhoudou 37, and Fandou 22 are the lines segregated to Phytophthora sojae."

Fig. 3

Detection of marker WZInDel11 in ten plants of soybean line Luodou 8816 Williams and Zheng 97196 are the controls of susceptible and resistant varieties, respectively. Luodou 8816-S indicates plant susceptible to Phytophthora sojae. Luodou 8816-R indicates plant resistance to P. sojae."

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