78份四川小麦育成品种(系)条锈病抗性鉴定与抗条锈病基因分子检测

doi:10.3724/SP.J.1006.2021.01061

Abstract

Abstract:

Sichuan province is one of the main origins of new races of the wheat stripe rust pathogen. To understand the resistance level of wheat cultivars (lines) bred in Sichuan since 2016 to the current predominant races of the pathogen and pathotypes and to clarify the distribution of resistance genes can provide a theoretical basis for the management of wheat stripe rust by breeding resistance wheat cultivars and wheat variety deployment in Sichuan. In this study, 78 Sichuan wheat cultivars (lines) were identified at seedling stage by two races CYR32 and CYR34. At adult plant stage in wheat, the mixture of the current predominant races of CYR32, CYR33, CYR34, G22-14, and G22 pathogenic groups virulent to wheat cultivar Guinong 22 were artificially inoculated to assess the resistance of the tested wheat. Nineteen resistance genes and QTLs, including QYr.nwafu-4BL, Yr5, Yr10, Yr15, Yr17, Yr18, Yr26, Yr28, Yr29, Yr30, Yr36, Yr39, Yr41, Yr48, Yr65, Yr67, Yr78, Yr80, and Yr81, were detected the presence of the resistance genes in all tested materials. The results showed that 60 (76.92%) of the 78 tested materials were resistant to CYR32, 40 (51.28%) resistant to CYR34, and 36 (46.15%) resistant to both races at seedling stage. Seventy-eight wheat materials were resistant to wheat stripe rust at adult stage, 4 out of which, including Mianmai 835, Shumai 1743, Shumai 1829, and Shumai 1868, were immune to the disease. Disease resistance identification showed that 42 (53.85%) exhibited adult plant resistance and 36 (46.15%) were all stage resistant to wheat stripe rust. Molecular detection indicated that 5, 5, 45, 2, 30, 5, 30, 39, 3, 2, 22, 8, 23, 6, and 24 carried the resistance gene QYr.nwafu-4BL, Yr15, Yr17, Yr18, Yr26, Yr28, Yr29, Yr30, Yr39, Yr41, Yr65, Yr67, Yr78, Yr80, and Yr81, respectively. Two to six resistance genes were detected in 24, 22, 11, 14, and 3 of the tested wheat materials, accounting for 94.87%. Yr5, Yr10, Yr36, and Yr48 were not detected in all wheat cultivars (lines), and only Xikemai 18, not detected any of the 19 resistance genes mentioned above, presumably, may carried other known or new resistance genes to wheat stripe rust. In summary, that resistance level of 78 wheat cultivars (lines) to wheat stripe rust was identified, and the resistance genes of these cultivars were identified. These results provide scientific basis for breeding durable resistant wheat cultivar.

Key words: stripe rust of wheat, wheat cultivars (lines), resistance evaluation, molecular detection

XI Ling, WANG Yu-Qi, ZHU Wei, WANG Yi, CHEN Guo-Yue, PU Zong-Jun, ZHOU Yong-Hong, KANG Hou-Yang. Identification of resistance to wheat and molecular detection of resistance genes to wheat stripe rust of 78 wheat cultivars (lines) in Sichuan province[J].Acta Agronomica Sinica, 2021, 47(7): 1309-1323.

Figures/Tables 6

Table 1

Fig. 1

Table 2

Evaluation of wheat resistance to stripe rust and detection of Yr genes in 78 Sichuan wheat cultivars (lines)"

序号 Number	品种 Variety	苗期反应型 Infection type in seedling stage		成株期2019WJ ^a 2019WJ ^a in adult-plant stage		抗性类型^b Resistance type ^b	Yr基因 Yr gene
序号 Number	品种 Variety	CYR32	CYR34	IT	DS	抗性类型^b Resistance type ^b	Yr基因 Yr gene
1	凡37123 Fan 37123	5	7	1	0	APR	Yr17+Yr81
2	31966	1	7	1	0	APR	Yr17+Yr81
3	绵麦161 Mianmai 161	5	5	1	0	ASR	Yr17+Yr26
4	绵麦319 Mianmai 319	5	5	2	5	ASR	QYr.nwafu-4BL+Yr17+Yr26+Yr65
5	绵麦827 Mianmai 827	5	5	2	5	ASR	Yr26+Yr30+Yr65+Yr78+Yr81
6	绵麦835 Mianmai 835	4	5	0	0	ASR	Yr15
7	绵麦902 Mianmai 902	6	6	3	5	ASR	Yr17+Yr78+Yr81
8	绵麦903 Mianmai 903	5	5	2	5	ASR	Yr17+Yr29+Yr30+Yr78+Yr80
9	绵麦905 Mianmai 905	6	0	1	0	ASR	Yr17+Yr28
10	绵麦906 Mianmai 906	2	6	1	0	ASR	QYr.nwafu-4BL+Yr17+Yr26+Yr39+Yr80
11	绵麦1419 Mianmai 1419	5	7	1	0	APR	Yr26+Yr30+Yr67+Yr78
12	绵麦1501 Mianmai 1501	5	7	3	10	APR	Yr17+Yr26+Yr29+Yr78+Yr81
13	绵麦5658 Mianmai 5658	1	7	1	0	APR	Yr17+Yr26+Yr30+Yr78+Yr80
14	绵麦5695 Mianmai 5695	1	4	2	5	ASR	Yr26+Yr78+Yr81
15	西科麦11 Xikemai 11	5	7	1	0	APR	Yr17+Yr26+Yr81
16	西科麦12 Xikemai 12	5	7	1	0	APR	Yr29+Yr30
17	西科麦18 Xikemai 18	5	7	1	0	APR	—
18	西科麦475 Xikemai 475	7	5	2	5	APR	Yr17+Yr26+Yr29+Yr30+Yr81
19	西科麦497 Xikemai 497	5	4	2	5	ASR	Yr29+Yr78
20	西科麦518 Xikemai 518	7	7	1	0	APR	Yr17+Yr29+Yr67
21	西科麦546 Xikemai 546	7	7	2	5	APR	Yr17+Yr29+Yr78
22	西科麦557 Xikemai 557	6	0	2	5	ASR	Yr15+Yr29
23	川农27 Chuannong 27	7	7	2	5	APR	Yr17+Yr39+Yr81
24	川农30 Chuannong 30	5	5	2	5	ASR	Yr17+Yr80
25	川农32 Chuannong 32	7	1	2	5	APR	Yr17+Yr39+Yr80+Yr81
26	川农38 Chuannong 38	5	7	1	0	APR	Yr17+Yr81
27	川农39 Chuannong 39	5	7	1	0	APR	Yr17+Yr30+Yr67
28	川农41 Chuannong 41	5	7	1	0	APR	Yr17+Yr81
29	川农42 Chuannong 42	7	7	1	0	APR	Yr17+Yr81
30	蜀麦691 Shumai 691	8	7	2	5	APR	Yr17+Yr29+Yr30+Yr67+Yr81
31	蜀麦1613 Shumai 1613	3	0	3	5	ASR	Yr17+Yr29+Yr67
32	蜀麦1671 Shumai 1671	5	7	2	5	APR	Yr26+Yr30+Yr65+Yr78
33	蜀麦1675 Shumai 1675	5	7	1	0	APR	Yr28+Yr29+Yr30+Yr81
34	蜀麦1743 Shumai 1743	5	5	0	0	ASR	Yr17+Yr29
35	蜀麦1783 Shumai 1783	5	1	3	5	ASR	Yr17+Yr29+Yr67
36	蜀麦1812 Shumai 1812	7	7	2	5	APR	Yr17+Yr81
37	蜀麦1820 Shumai 1820	7	8	4	10	APR	Yr17+Yr29+Yr78
38	蜀麦1821 Shumai 1821	7	7	1	0	APR	Yr26+Yr30+Yr78
39	蜀麦1829 Shumai 1829	0	1	0	0	ASR	Yr15+Yr17
40	蜀麦1843 Shumai 1843	7	6	1	0	APR	QYr.nwafu-4BL+Yr17+Yr29
41	蜀麦1862 Shumai 1862	7	7	1	0	APR	Yr26+Yr30+Yr65+Yr78
42	蜀麦1868 Shumai 1868	2	0	0	0	ASR	Yr15+Yr17+Yr30+Yr41+Yr78
43	蜀麦1871 Shumai 1871	5	5	1	0	ASR	Yr17+Yr29+Yr30+Yr67+Yr81
44	蜀麦1885 Shumai 1885	6	5	3	10	ASR	Yr17+Yr29+Yr67
45	川麦83 Chuanmai 83	5	0	2	5	ASR	Yr17+Yr29
46	川麦84 Chuanmai 84	5	7	2	5	APR	QYr.nwafu-4BL+Yr30+Yr65+Yr78
47	川麦86 Chuanmai 86	5	7	1	0	APR	Yr26+Yr29+Yr30+Yr78
48	川麦87 Chuanmai 87	7	7	2	5	APR	Yr17+Yr30+Yr80+Yr81
49	川麦88 Chuanmai 88	7	7	2	5	APR	Yr30
50	川麦93 Chuanmai 93	6	7	3	5	APR	Yr26+Yr28+Yr29+Yr30+Yr65+Yr81
51	川麦98 Chuanmai 98	5	5	1	0	ASR	Yr17+Yr28+Yr29+Yr81
52	川麦603 Chuanmai 603	4	7	1	0	APR	Yr26+Yr29+Yr78
53	川麦604 Chuanmai 604	5	7	1	0	APR	Yr17+Yr26+Yr30+Yr65
54	川麦605 Chuanmai 605	0	1	2	5	ASR	QYr.nwafu-4BL+Yr26+Yr29+Yr30+Yr65
55	川麦606 Chuanmai 606	5	7	1	0	APR	Yr30+Yr78
56	川麦607 Chuanmai 607	7	7	1	0	APR	Yr17+Yr26+Yr78
57	川麦608 Chuanmai 608	5	5	1	0	ASR	Yr17+Yr26+Yr29+Yr65+Yr78
58	川麦609 Chuanmai 609	5	7	1	0	APR	Yr17+Yr26+Yr29+Yr65+Yr78
59	川麦610 Chuanmai 610	1	4	1	0	ASR	Yr30+Yr65
60	川麦611 Chuanmai 611	5	0	1	0	ASR	Yr29
61	川麦613 Chuanmai 613	2	7	1	5	APR	Yr17+Yr29+Yr81
62	川麦801 Chuanmai 801	5	7	1	0	APR	Yr17+Yr18+Yr26+Yr30+Yr65+Yr81
63	川麦802 Chuanmai 802	5	5	1	0	ASR	Yr17+Yr30+Yr81
64	川麦803 Chuanmai 803	5	7	1	5	APR	Yr17+Yr26+Yr30+Yr65+Yr78
65	川麦901 Chuanmai 901	7	7	2	5	APR	Yr29+Yr65
66	川麦905 Chuanmai 905	4	5	3	5	ASR	Yr26+Yr30+Yr65
67	川麦906 Chuanmai 906	5	5	2	5	ASR	Yr26+Yr28+Yr29+Yr30+Yr65+Yr81
68	川麦907 Chuanmai 907	5	5	1	0	ASR	Yr30+Yr65
69	川麦1456 Chuanmai 1456	6	5	2	5	ASR	Yr18+Yr26+Yr30+Yr41+Yr78
70	川麦1546 Chuanmai 1546	5	5	4	10	ASR	Yr30+Yr65
71	川麦1557 Chuanmai 1557	7	7	2	5	APR	Yr30+Yr65
72	川麦1580 Chuanmai 1580	5	5	1	0	ASR	Yr30+Yr65
73	川麦1603 Chuanmai 1603	5	5	1	0	ASR	Yr30+Yr65
74	川麦1648 Chuanmai 1648	5	5	1	0	ASR	Yr17+Yr26+Yr30
75	川麦1650 Chuanmai 1650	5	7	1	0	APR	Yr17+Yr26+Yr30
76	川麦1694 Chuanmai 1694	7	5	1	0	APR	Yr26+Yr30
77	川麦1699 Chuanmai 1699	5	5	2	5	ASR	Yr26+Yr30+Yr65
78	川麦1747 Chuanmai 1747	0	0	1	0	ASR	Yr15+Yr29+Yr30

Table 2

Fig. 2

Fig. 3

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Yr基因 Yr gene	类别^a Type ^a	分子标记 Molecular marker	引物序列 Primer sequence (5′?3′)	参考文献 Reference
QYr.nwafu-4BL	KASP	AX-110963704	GAAGGTGACCAAGTTCATGCTTCGTTTGCCTATGGTTCAAAAC	[31]
			GAAGGTCGGAGTCAACGGATTTCGTTTGCCTATGGTTCAAAAG
			TCTCCATGACACGGGAGGAT
Yr5	DM	Yr5_B	GGGAACACTTCACGATCA	[32]
			AATTCCTTCATGCCTTCC
	DM	Yr5_A	CGCTTAATTCCCCTTCCTTC	[32]
			TGGCTCCTTATTCGTTCTCTTTC
	KASP	Yr5F	GAAGGTGACCAAGTTCATGCTGCGCCCCTTTTCGAAAAAATA	[32]
		Yr5H	GAAGGTCGGAGTCAACGGATTCTAGCATCAAACAAGCTAAATA
		Yr5R	ATGTCGAAATATTGCATAACATGG
Yr10	AFLP	SC200	CTGCAGAGTGACATCATACA	[33]
			TCGAACTAGTAGATGCTGGC
	DM	Yr10-5	GGAAATGTGGCGGAGTACCA	[34]
			CGGAAGGGAGAACCACTGTC
	DM	Yr10-6	CAGCTTGACAAGGGCGAGTA	[34]
			CGCTTGTCGCCAATTCCAAA
Yr15	DM	Y15K1_F2	GGAGATAGAGCACATTACAGAC	[35]
		uhw301R	GGAGATAGAGCACATTACAGAC
	DM	WJL2F	CCTTGTGTGCTACCAGGGTT	[36]
		WJL2R	GGAACTCAAGCCCTTCTGCT
	DM	WJL3F	AAAAGAGCTCGCCTCCTACG	[37]
		WJL3R	GCCATGATGAGATCGGGAGG
Yr17	SCAR	SC2372	AGGGGCTACTGACCAAGGCT	[38]
			TGCAGCTACAGCAGTATGTACACAAAA
Yr18	STS	csLV34	GTTGGTTAAGACTGGTGATGG	[39]
			TGCTTGCTATTGCTGAATAGT
	Cssfr1	L34DINT9F	TTGATGAAACCAGTTTTTTTTCTA	[39]
		L34PLUSR	GCCATTTAACATAATCATGATGGA
	KASP	Lr34-KASP-E11	GAAGGTGACCAAGTTCATGCTGGGAGCATTATTTTTTTCCATCA	[40]
			GAAGGTCGGAGTCAACGGATTGGGAGCATTATTTTTTTCCATCT
			AGCGAATCCAGTATGGAAAT
Yr26	STS	Xwe173	GGGACAAGGGGAGTTGAAGC	[41]
			GAGAGTTCCAAGCAGAACAC
	STS	Xwe33	TAAACCAAGTCCCCCAAA	[41]
			GGAGTCCATCTTCACCGA
Yr28	InDel	ZCZp343F	TGTGTCATGTTTGGTCGATAGG	[42]
		ZCZp342R	TCCTCCCTTGTAGCTTCACG

Yr基因 Yr gene	类别^a Type ^a	分子标记 Molecular marker	引物序列 Primer sequence (5′?3′)	参考文献 Reference
Yr28	DM	P175	GCACCGTCCTTCATCTCAGT	[42]
		P176	TGCTTTTCCCCGTATCCCTT
Yr29	CAPS	csLV46	CGAGACGTCGTCTTCTCTAAC	[43]
			GTGTATGTGTTGATTCTCCTCG
Yr30	SSR	Xgwm533	GTTGCTTTAGGGGAAAAGCC	[44]
			AAGGCGAATCAAACGGAATA
Yr36	DM	WKS1_150F	ATGGAGCTCCCACGAAACAAAC	[45]
		WKS1_620R	ACCTCCATGTTGCTCGCATTTGCT
	DM	WKS1_182F	TAGCTCTTCGTGGCCAAAGG	[45]
		WKS1_150R	TGGCCACGAAGAGCTAAAGG
Yr39	SSR	Xgwm131	AATCCCCACCGATTCTTCTC	[46]
			AGTTCGTGGGTCTCTGATGG
Yr41	SSR	Xgwm410	GCTTGAGACCGGCACAGT	[47]
			CGAGACCTTGAGGGTCTAGA
	SSR	Xgwm374	ATAGTGTGTTGCATGCTGTGTG	[47]
			TCTAATTAGCGTTGGCTGCC
Yr48	SSR	Xwmc727	CATAATCAGGACAGCCGCAC	[48]
			TAGTGGCCTGATGTATCTAGTTGG
	SSR	Xwms291	CATCCCTACGCCACTCTGC	[48]
			AATGGTATCTATTCCGACCCG
Yr65	SSR	Xgwm18	GGTTGCTGAAGAACCTTATTTAGG	[49]
			TGGCGCCATGATTGCATTATCTTC
Yr67	SSR	Xbarc182	CCATGGCCAACAGCTCAAGGTCTC	[50]
			CGCAAAACCGCATCAGGGAAGCACCAAT
	SSR	Xcfa2040	TCAAATGATTTCAGGTAACCACTA	[50]
			TTCCTGATCCCACCAAACAT
Yr78	KASP	IWA7257	GAAGGTGACCAAGTTCATGCTAGACCCTACGACGTTAGCGC	[51]
			GAAGGTCGGAGTCAACGGATTAGACCCTACGACGTTAGCGA
			ATTGGAATCAGCTGGGTCAT
Yr80	KASP	KASP_53113	GAAGGTGACCAAGTTCATGCTTGTACAATGACTCCTCGACTAACA	[52]
			GAAGGTCGGAGTCAACGGATTTGTACAATGACTCCTCGACTAACG
			GCCACGCAATATCACCATCG
Yr81	KASP	KASP_3077	GAAGGTGACCAAGTTCATGCTATTCCAAAGTAATTGGCAACAGGTTCA	[53]
			GAAGGTCGGAGTCAACGGATTCCAAAGTAATTGGCAACAGGTTCG
			TGTGGAGCGTGACAATGAGGAAGTT

Identification of resistance to wheat and molecular detection of resistance genes to wheat stripe rust of 78 wheat cultivars (lines) in Sichuan province

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[6]	Ming-Gang XIAO, Feng-Jing SONG, Bing SUN, Xin ZUO, Guang-Shan ZHAO, Ai-Hua XIN, Zhu-Gang LI. Exploration of Foreign Maize Inbred Lines with Broad Spectrum Resistance to Northern Corn Leaf Blight and Preliminary Identification of Resistance Genes [J]. Acta Agronomica Sinica, 2018, 44(04): 614-619.
[7]	MAO Yan-Zhi,LI Chun-Jie,HU Yan-Feng,HUA Cui,YOU Jia,WANG Xin-Peng,LIU Xi-Cai,YANG Geng-Bin,WANG Cong-Li. Resistance Evaluation of Potato Cultivars and Germplasms to Meloidogyne hapla in Heilongjiang Province [J]. Acta Agron Sin, 2017, 43(12): 1864-1869.
[8]	LI Wen-Feng,WANG Xiao-Fan,HUANG Ying-Fun*,ZHANG Rong-Yue,SHAN Hong-Li,LUO Zhi-Ming,YIN Jiong. Identification of Resistance to Brown Rust and Molecular Detection of Bru1 Gene in 101 Main Sugarcane Breeding Parents in China [J]. Acta Agron Sin, 2016, 42(09): 1411-1416.
[9]	LI Wen-Feng, WANG Xiao-Yan, HUANG Ying-Kun, ZHANG Rong-Yue, SHAN Hong-Li, YIN Jiong,LUO Zhi-Ming. Identification of Resistance to Brown Rust and Molecular Detection of Bru1* Gene in 31 Wild Core Sugarcane Germplasms [J]. Acta Agron Sin, 2015, 41(05): 806-812.
[10]	XING Xiao-Ping,YUAN Hong-Xia,SUN Jun-Wei,ZHANG Jie,SUN Bing-Jian,LI Hong-Lian. Resistance to Two Species of Cereal Cyst Nematode and Its Evaluation Methods in 47 Major Wheat Cultivars in Henan Province, China [J]. Acta Agron Sin, 2014, 40(05): 805-815.
[11]	LI Ai-Hong,PAN Cun-Hong,DAI Zheng-Yuan,XIAO Ning,YU Ling,LI Yu-Hong,ZHANG Xiao-Xiang,ZHANG Hong-Xi,PAN Xue-Biao. Using Marker-Assisted Selection to Improve the Resistance to Rice Black-Streaked Dwarf Viral Disease of Huaidao 5, an Elite Japonica Rice Cultivar in Jiangsu [J]. Acta Agron Sin, 2012, 38(10): 1775-1781.
[12]	DANG Liang,WANG Ai-Yun,XU Hui-Jun,ZHU Xiu-Liang,DU Li-Pu,SHAO Yan-Jun,ZHANG Zeng-Yan. Development and Characterization of GmPGIP3 Transgenic Wheat Yangmai 18 with Enhanced Resistance to Common Root Rot [J]. Acta Agron Sin, 2012, 38(10): 1833-1838.
[13]	CAO Shi-Qi, ZHANG Bo, LI Meng-Ju, XU Shi-Chang, JIA Hui-Sheng, JIN She-Lin, GU Qiu-Zhen, HUANG Jin, JIN Meng-An, CHANG Xun-Wu. Postulation of Stripe Rust Resistance Genes and Analysis of Adult Resistance in 50 Wheat Varieties (Lines) in Gansu Province [J]. Acta Agron Sin, 2011, 37(08): 1360-1371.
[14]	WANG Ling, HUANG Wen-Wen, LIU Lian-Meng, FU Qiang, HUANG Shi-Wen. Evaluation of Resistance to Sheath Blight (Rhizoctonia solani) in Some Indica Hybrid Rice from Southern China [J]. Acta Agron Sin, 2011, 37(02): 263-270.
[15]	ZUO Shi-Min,WANG Zi-Bin,CHEN Xi-Jun,GU Fang,ZHANG Ya-Fang,CHEN Zong-Xiang,PAN Xue-Biao. Evaluation of Resistance of a Novel Rice Germplasm YSBR1 to Sheath Blight [J]. Acta Agron Sin, 2009, 35(4): 608-614.