Welcome to Acta Agronomica Sinica,

Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (04): 614-619.doi: 10.3724/SP.J.1006.2018.00614

• RESEARCH NOTES • Previous Articles     Next Articles

Exploration of Foreign Maize Inbred Lines with Broad Spectrum Resistance to Northern Corn Leaf Blight and Preliminary Identification of Resistance Genes

Ming-Gang XIAO1,2(), Feng-Jing SONG3, Bing SUN2, Xin ZUO4, Guang-Shan ZHAO5, Ai-Hua XIN2, Zhu-Gang LI2,*()   

  1. 1 Postdoctoral Programme, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    2 Heilongjiang Province Cold Region Crop Physiology and Ecology Laboratory / Key Laboratory of Crop Molecular Design and Germplasm Innovation in Heilongjiang Province Tillage and Cultivation Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    3 Qingdao Academy of Agricultural Science, Qingdao 266109, Shandong, China
    4 Ruaral Energy Sources Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    5 Agricultural Technology Extension Station of Jiamusi City, Jiamusi 154002, Heilongjiang, China
  • Received:2017-08-30 Accepted:2018-01-08 Online:2018-01-30 Published:2018-01-30
  • Contact: Zhu-Gang LI E-mail:xiaoyang8076@163.com;lizhugang@163.com
  • Supported by:
    This study was supported by Heilongjiang Postdoctoral Fund (LB-Z14186).

Abstract:

From 2014 to 2016, forty-three maize inbred lines introduced from the United States, France, Russia and Germany, were identified and evaluated for resistance to northern corn leaf blight (NCLB). The results showed that seven, one and six maize inbreds were highly resistant, resistant or moderately resistant to NCLB, accounting for 32.6%. Exserohilum turcicum was used to test the F2 populations for analyzing the inheritance of NCLB resistance in the seven highly resistance maize inbred lines. Genetic analysis suggested that A04, F02, F05, and R01 of these inbred lines probably carried a single dominant gene conferring their resistance to NCLB. The reaction patterns to isolates 0, 1, 2, N, and 123N of E. turcicum showed that new genes resistance to NCLB were most likely present in A04, F02, F05, and R01. The results provided useful information on the introduction of maize germplasms and genetic improvement for resistance to NCLB.

Key words: maize inbred lines, northern corn leaf blight, resistance evaluation, inheritance

Fig. 1

Distribution of northern corn leaf blight resistance score for foreign maize inbred lines in 2014HR: highly resistant; R: resistant; MR: moderately resistant; S: susceptible; HS: highly susceptible."

Fig. 2

Field phenotype of foreign maize inbred lines highly resistant and highly sensitive to northern corn leaf blightA04, G03: inbred lines; HR: highly resistant; HS: highly susceptible."

Table 1

Identification of resistance to northern corn leaf blight of 14 foreign inbred lines across years"

自交系
Inbred lines
来源
Origin
2014 2015 2016
病斑面积
Lesion area
(%)
抗性评价
Resistance
evaluation
病斑面积
Lesion area
(%)
抗性评价
Resistance
evaluation
病斑面积
Lesion area
(%)
抗性评价
Resistance
evaluation
A02 美国America 15 MR 10 R 17 MR
A04 美国America 1 HR 1 HR 1 HR
A05 美国America 12 MR 12 MR 12 MR
A08 美国America 23 MR 20 MR 25 MR
A11 美国America 5 HR 5 HR 5 HR
A18 美国America 11 MR 11 MR 10 R
A20 美国America 25 MR 25 MR 20 MR
A24 美国America 0 HR 0 HR 0 HR
A28 美国America 13 MR 15 MR 10 R
F02 法国France 1 HR 3 HR 3 HR
F03 法国France 9 R 7 R 9 R
F05 法国France 2 HR 1 HR 1 HR
R01 俄罗斯Russia 4 HR 4 HR 4 HR
G02 德国Germany 0 HR 1 HR 0 HR

Table 2

Reactions of seven F2 populations to Exserohilum turcicum of northern corn leaf blight"

杂交组合
Cross
抗病植株数
R
感病植株数
S
分离比
χ2
P
P-value
获白/A04 Huobai/A04 448 132 1.5540 0.2125
获白/A11 Huobai/A11 416 168 4.4201 0.0355
获白/A24 Huobai/A24 400 160 3.8095 0.0510
获白/F02 Huobai/F02 438 130 1.3521 0.2449
获白/F05 Huobai/F05 438 142 0.0828 0.7736
获白/R01 Huobai/R01 454 158 0.2179 0.6407
获白/G02 Huobai/G02 432 172 3.8940 0.0485

Table 3

Infection types in the four foreign inbred lines to isolates 0, 1, 2, N, and 123N of northern corn leaf blight"

自交系
Inbred line
小种鉴定结果Identification of species
0 1 2 N 123N
A619 S S S S S
A619Ht1 R S R R S
A619Ht2 R R S R S
A619Ht3 R R R R S
A619HtN R R R S S
A04 R R R R R
F02 R R R R R
F05 R R R R R
R01 R R R R R
[1] 王晓鸣, 石洁, 晋齐鸣, 李晓, 孙世贤. 玉米病虫害田间手册. 北京: 中国农业科学技术出版社, 2010. pp 1, 250, 264-265
Wang X M, Shi J, Jin Q M, Li X, Sun S X.Field Manual of Maize Pests and Diseases. Beijing: China Science and Technology Press, 2010. pp 1, 250, 264-265 (in Chinese)
[2] 温义鹏, 李成军, 于培洋, 从方志, 郭宝贵, 朱秀森, 姜傅俊, 刘伟. 不同玉米自交系对大斑病和灰斑病抗性分析. 玉米科学, 2012, 20(1): 135-137
Wen Y P, Li C J, Yu P Y, Cong F Z, Guo B G, Zhu X S, Jiang F J, Liu W.Resistance analysis on the northern leaf blight and the gray leaf spot of the corn inbred lines.J Maize Sci, 2012, 20(1): 135-137 (in Chinese with English abstract)
[3] 张小利. 玉米应答大斑菌侵染的蛋白质组学与抗南方锈病新基因的挖掘. 中国农业科学院博士学位论文,北京, 2013
Zhang X L.Proteomic Analysis of Maize Infected by Setosphaeria turcica and Discovery of New Resistance Gene to Southern Corn Rust. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2013 (in Chinese with English abstract)
[4] Wang X M, Zhang Y H, Xu X D, Li H J, Wu X F, Zhang S H, Li X H.Evaluation of maize inbred lines currently used in Chinese breeding programs for resistance to six foliar diseases.Crop J, 2014, 2: 213-222
[5] 王晓鸣, 晋齐鸣, 石洁, 王作英, 李晓. 玉米病害发生现状与推广品种抗性对未来病害发展的影响. 植物病理学报, 2006, 36: 1-11
Wang X M, Jin Q M, Shi J, Wang Z Y, Li X.The status of maize diseases and the possible effect of variety resistance occurrence in the future.Acta Phytopathol Sin, 2006, 36: 1-11 (in Chinese with English abstract)
[6] Van Staden D, Lambert C A, Lehmensiek A.SCAR markers for the Ht1, Ht2, Ht3 and Htn1 resistance genes in maize. Maize Genet Conf Abs, 2001, 43: 134
[7] Chung C L, Jamann T, Longfellow J, Nelson R.Characterization and fine-mapping of a resistance locus for northern leaf blight in maize bin 8.06.Theor Appl Genet, 2010, 121: 205-227
[8] Simcox K D, Bennetzen J L.The use of molecular markers to study Setosphaeria turcica resistance in maize.Phytopathology, 1993, 83: 1326-1330
[9] Robbins J W, Warren H.Inheritance of resistance to Exserohilum turcicum in PI 209135, “Mayorbela” variety of maize.Maydica, 1993, 38: 209-213
[10] Ogliari J B, Guimarães M A, Camargo L E A. Chromosomal locations of the maize ( Zea mays L.) HtP and rt genes that confer resistance to Exserohilum turcicum. Genet Mol Biol, 2007, 30: 630-634
[11] Welz H, Geiger H.Genes for resistance to northern corn leaf blight in diverse maize populations.Plant Breed, 2000, 119: 1-14
[12] Martin T, Biruma M, Fridborg I, Okori P, Dixelius C.A highly conserved NB-LRR encoding gene cluster effective against Setosphaeria turcica in sorghum.BMC Plant Biol, 2011, 11: 151
[13] 王玉萍, 王晓鸣, 马青. 我国玉米大斑病菌生理小种组成变异研究. 玉米科学, 2007, 15(2): 123-126
Wang Y P, Wang X M, Ma Q.Races of Exserohihun turcicum, causal agent of northern leaf blight in China. J Maize Sci, 2007, 15(2): 123-126 (in Chinese with English abstract)
[14] 杨继良, 王斌. 玉米大斑病抗性遗传的研究进展. 遗传, 2002, 24: 501-506
Yang J L, Wang B.The research advancement on genetics of resistance to Exserohihun turcicum in maize.Genetics(Beijing), 2002, 24: 501-506 (in Chinese with English abstract)
[15] 赵桂东, 刘荆, 陆化森, 王伟新. 夏玉米大斑病发生规律及影响病害消长因素的研究. 玉米科学, 1995, 3(2): 79-80
Zhao G D, Liu J, Lu H S, Wang W X.Study on the occurrence regularity and the factors affecting the growth and decline of summer northern corn leaf blight.Maize Sci, 1995, 3(2): 79-80 (in Chinese with English abstract)
[16] 赵书文, 杨秀林, 郭东. 玉米大斑病的流行原因与综合治理措施. 中国植保导刊, 2005, 25(3): 10-12
Zhao S W, Yang X L, Guo D.Epidemic causes of Exserochilum turcicum and its integrated management measures. China Plant Prot, 2005, 25(3): 10-12 (in Chinese with English abstract)
[17] Wisser R J, Balint-Kurti P J, Nelson R J. The genetic architecture of disease resistance in maize: a synthesis of published studies.Phytopathology, 2006, 96: 120-129
[18] Ali F, Yan J B.The phenomenon of disease resistance in maize and the role of molecular breeding in defending against global threat.J Int Plant Biol, 2012, 54: 134-151
[19] Parlevliet J E.Durability of resistance gaginst fungal, bacterial and viral pathogens; present situation.Euphytica, 2002, 124: 147-156
[20] Chung C L, Longfellow J, Walsh E K, Kerdieh Z, Esbroeck G V, Balint-Kurti P, Nelson R J.Resistance loci affecting distinct stages of fungal pathogenesis: use of introgression lines for QTL mapping and characterization in the maize Setosphaeria turcica pathosystem.BMC Plant Biol, 2010, 10: 103
[21] Chung C L, Poland J, Kump K, Benson J, Longfellow J, Walsh E, Balint-Kurti P, Nelson R.Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize.Theor Appl Genet, 2011, 123: 307-326
[22] Ploand J A, Bradbury P J, Buckler E S, Nelson R J.Genome-wide nested association mapping of quantitative resistance to northern leaf blight in maize. Proc Natl Acad Sci USA, 2011, 108: 6893-6898
[23] Chen G S, Wang X M, Long S S, Jaqueth J, Li B L, Yan J B, Ding J Q.Mapping of QTL conferring resistance to northern corn leaf blight using high-density SNPs in maize. Mol Breed, 2016, 36: 4
[24] Chung C L, Jamann T, Longfellow J, Nelson R.Characterization and fine-mapping of a resistance locus for northern leaf blight in maize bin 8.06.Theor Appl Genet, 2010, 121: 205-227
[25] Jamann T M, Poland J A, Kolkman J M, Smith L G, Nelson R J.Unraveling genomic complexity at a quantitative disease resistance locus in maize.Genetics, 2014, 198: 333-334
[26] Badu-Apraku B, Gracen V, Bergstrom G.A major gene for resistance to anthracnose stalk rot in maize.Phytopathology, 1987, 77: 957-959
[27] Jung M, Weldekidan T, Schaff D, Paterson A, Tingey S, Hawk J.Generation-means analysis and quantitative trait locus mapping of anthracnose stalk rot genes in maize.Theor Appl Genet, 1994, 89: 413-418
[1] WANG Jing-Tian, ZHANG Ya-Wen, DU Ying-Wen, REN Wen-Long, LI Hong-Fu, SUN Wen-Xian, GE Chao, ZHANG Yuan-Ming. SEA v2.0: an R software package for mixed major genes plus polygenes inheritance analysis of quantitative traits [J]. Acta Agronomica Sinica, 2022, 48(6): 1416-1424.
[2] YANG Xin, LIN Wen-Zhong, CHEN Si-Yuan, DU Zhen-Guo, LIN Jie, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Li-Wu. Molecular identification of a geminivirus CoYVV and screening of resistant germplasms in jute [J]. Acta Agronomica Sinica, 2022, 48(3): 624-634.
[3] XI Ling, WANG Yu-Qi, ZHU Wei, WANG Yi, CHEN Guo-Yue, PU Zong-Jun, ZHOU Yong-Hong, KANG Hou-Yang. Identification of resistance to wheat and molecular detection of resistance genes to wheat stripe rust of 78 wheat cultivars (lines) in Sichuan province [J]. Acta Agronomica Sinica, 2021, 47(7): 1309-1323.
[4] HUANG Bing-Yan, SUN Zi-Qi, LIU Hua, FANG Yuan-Jin, SHI Lei, MIAO Li-Juan, ZHANG Mao-Ning, ZHANG Zhong-Xin, XU Jing, ZHANG Meng-Yuan, DONG Wen-Zhao, ZHANG Xin-You. Genetic analysis of fat content based on nested populations in peanut (Arachis hypogaea L.) [J]. Acta Agronomica Sinica, 2021, 47(6): 1100-1108.
[5] ZHAO Xu-Yang, YAO Fang-Jie, LONG Li, WANG Yu-Qi, KANG Hou-Yang, JIANG Yun-Feng, LI Wei, DENG Mei, LI Hao, CHEN Guo-Yue. Evaluation of resistance to stripe rust and molecular detection of resistance genes of 93 wheat landraces from the Qinghai-Tibet spring and winter wheat zones [J]. Acta Agronomica Sinica, 2021, 47(10): 2053-2063.
[6] ZHANG An-Ning,LIU Yi,WANG Fei-Ming,XIE Yue-Wen,KONG De-Yan,NIE Yuan-Yuan,ZHANG Fen-Yun,BI Jun-Guo,YU Xin-Qiao,LIU Guo-Lan,LUO Li-Jun. Pyramiding and evaluation of brown planthopper resistance genes in water-saving and drought-resistance restorer line [J]. Acta Agronomica Sinica, 2019, 45(11): 1764-1769.
[7] Zhu LI, Li-Ping XU, Ya-Chun SU, Qi-Bin WU, Wei CHENG, Ting-Ting SUN, Shi-Wu GAO. Analysis of Brown Rust Resistance Inheritance Based on Field Phenotypes and Detection of Bru1 Gene in Sugarcane [J]. Acta Agronomica Sinica, 2018, 44(02): 306-312.
[8] MAO Yan-Zhi,LI Chun-Jie,HU Yan-Feng,HUA Cui,YOU Jia,WANG Xin-Peng,LIU Xi-Cai,YANG Geng-Bin,WANG Cong-Li. Resistance Evaluation of Potato Cultivars and Germplasms to Meloidogyne hapla in Heilongjiang Province [J]. Acta Agron Sin, 2017, 43(12): 1864-1869.
[9] SUN Ru-Jian,SUN Bin-Cheng,ZHANG Qi,HU Xing-Guo,GUO Rong-Qi,GUO Bing-Fu,MA Yan-Song,YU Ping,ZHANG Xiao-Li,CHAI Shen,ZHANG Wan-Hai,QIU Li-Juan. Correlation between Resistance to Glyphosate and Genetic Background in Transgenic CP4-EPSPs Gene Soybean Progeny [J]. Acta Agron Sin, 2017, 43(03): 324-331.
[10] LIN Ting-Ting,WANG Jian-Jun,WANG Li,CHEN Xuan,HOU Xi-Lin,LI Ying. Major Gene Plus Polygene Inheritance Analysis of Vitamin C Content in Non-heading Chinese Cabbage [J]. Acta Agron Sin, 2014, 40(10): 1733-1739.
[11] CHEN Chun-Mei,GAO Ju-Lin*,SU Zhi-Jun,YU Xiao-Fang,HU Shu-Ping,ZHAO Xiao-Liang. Relationship between Leaf Photosynthetic Parameters and Drought Resistance at Silking Stage in Maize Inbred Lines [J]. Acta Agron Sin, 2014, 40(09): 1667-1676.
[12] ZHOU Qing-Yuan,CUI Cui,YIN Tao,CHEN Dong-Liang,ZHANG Zheng-Sheng,LI Jia-Na. Genetic Analysis of Silique Length Using Mixture Model of Major Gene Plus Polygene in Brassica napus L. [J]. Acta Agron Sin, 2014, 40(08): 1493-1500.
[13] DU Li-Fen,LI Ming-Fei,LIU Lu-Xiang,WANG Chao-Jie,LIU Yang,XU Xi-Tang,ZOU Shu-Fang,XIE Yan-Zhou,WANG Cheng-She. Physiological Characteristics and Genetic Analysis on a Spotted-Leaf Wheat Derived from Chemical Mutation [J]. Acta Agron Sin, 2014, 40(06): 1020-1026.
[14] XING Xiao-Ping,YUAN Hong-Xia,SUN Jun-Wei,ZHANG Jie,SUN Bing-Jian,LI Hong-Lian. Resistance to Two Species of Cereal Cyst Nematode and Its Evaluation Methods in 47 Major Wheat Cultivars in Henan Province, China [J]. Acta Agron Sin, 2014, 40(05): 805-815.
[15] WANG Li-Xia,CHENG Xu-Zhen,WANG Su-Hua,LIU Yan. Inheritance of Several Traits in Mungbean (Vigna radiata) [J]. Acta Agron Sin, 2013, 39(07): 1172-1178.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!