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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (03): 324-331.doi: 10.3724/SP.J.1006.2017.00324

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Correlation between Resistance to Glyphosate and Genetic Background in Transgenic CP4-EPSPs Gene Soybean Progeny

SUN Ru-Jian,SUN Bin-Cheng,ZHANG Qi,HU Xing-Guo,GUO Rong-Qi,GUO Bing-Fu,MA Yan-Song,YU Ping,ZHANG Xiao-Li,CHAI Shen,ZHANG Wan-Hai,QIU Li-Juan   

  1. 1 Hulun Buir Institution of Agricultural Sciences,Zhalantun 162650,Inner Mongolia;2 The National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology (Beijing) / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Northeast Agricultural University, Harbin 150030, China; 4 Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2016-06-18 Revised:2016-11-03 Online:2017-03-12 Published:2016-11-15
  • Contact: 邱丽娟, E-mail: qiulijuan@caas.cn; 张万海, E-mail: zwh00001@sina.com E-mail:sunrujian321@126.com
  • Supported by:

    This study was supported by the Major Project of China on New Varieties of GMO Cultivation (ZX2008004-001, ZX2011004-001, ZX2013004-001, ZX2014004-001).

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Abstract:

The first Roundup Ready (RR) soybean (GTS40-3-2) carrying CP4-EPSPs is one of the transgenic crops that have the longest application time and largest planting acreage worldwide. However, it has not been commercialized and has less report in China. In this study, six RR soybean lines derived from GTS40-3-2 were hybridized to create four combinations. We used resistance grading and relative plant height in identifying the resistance to glyphosate of parents and F2:3 progeny, to investigate the correlation between resistance of the progeny and genetic background. The growth inhibition of transgenic parents and progeny at seeding stage was not significant when the glyphosate treatment concentration was 1230 g a.i. hm–2, while it was significant when the glyphosate concentration was 3690 g a.i. hm–2 and 4920 g a.i. hm–2. ZLHJ06-1568, ZLHJ10-713, and ZLHJ06-698 among the six parents had the more glyphosate resistance relatively. The progeny of ZLHJ10-713×ZLHJ06-698 combination had the least inhibition of plant height showing highest resistance to glyphosate in all four F2:3 combinations. The progeny had better resistance to glyphosate than their parents showing better heterosis. The glyphosate resistance of progeny was positively correlated with that of parents. Difference of glyphosate resistance was found in progeny among different combinations because of the interaction between parents. Our study indicates that the expression of glyphosate resistance gene is closely related to the genetic background in soybean, providing a theoretical evidence for target gene selection in developing new varieties by using transgenic germplasm.

Key words: Transgenic soybean, Glyphosate-resistant characteristics, Inheritance

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