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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (03): 332-342.doi: 10.3724/SP.J.1006.2017.00332

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Pm52: Effectiveness of the Gene Conferring Resistance to Powdery Mildew in Wheat Cultivar Liangxing 99

ZOU Jing-Wei1,2,QIU Dan1,2,SUN Yan-Ling2,ZHENG Chao-Xing3,LI Jing-Ting4,WU Pei-Pei2,WU Xiao-Fei2,WANG Xiao-Ming2,ZHOU Yang2,LI Hong-Jie2,*   

  1. 1 College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;   3 College of Chemistry and Environment Engineering, Pingdingshan College, Pingdingshan 467000, China; 4 College of Life Science, Beijing Normal University, Beijing 100875, China
  • Received:2016-06-11 Revised:2016-09-18 Online:2017-03-12 Published:2016-09-29
  • Contact: LI Hongjie, E-mail: lihongjie@caas.cn E-mail:zoujingwei2013@qq.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31471491 and 31501310), the National Major Project for Developing New GM Crops (2014ZX0800906B-003), and the Agricultural Science and Technology Innovation Program of CAAS.

Abstract:

Liangxing 99 is a powdery mildew-resistant winter wheat cultivar adapted to the Yellow–Huai Rivers Valley Facultative Wheat Zone and the Northern Winter Wheat Zone. Its powdery mildew resistance is conferred by gene Pm52 on chromosome 2BL. A total of 123 isolates of Blumeria graminis f. sp. tritici collected from different wheat producing regions were used in assessment of disease resistance, and Liangxing 99 was resistant to 80% of them. During the five consecutive growing seasons from 2012 to 2016, Liangxing 99 was immune or highly resistant to the mixture of Bgt isolate inoculated at the adult plant stage. A gene-specific marker Xgwm120 closedly linked to Pm52 and 27 Bgt isolates were used to analyze ten wheat cultivars with Liangxing 99 as a parent. Heng 4568, Hannong 2312, Zhongxinmai 99, and DH51302 may inherit Pm52 from Liangxing 99, while the powdery mildew resistance genes of Zhengmai 369 and Jimai 729 may differ from Pm52. Shi U09-4366, XR4429, Heng 10-5039, and Nongda 3486 were susceptible to all the Bgt isolates examined and did not carry gene Pm52. The responses of these cultivars at the adult plant stage were consistent with those at the seedling stage. Results from this study are favorable to facilitate the effective application of Pm52 from Liangxing 99 in breeding programs and the production of wheat.

Key words: Pm52, Liangxing 99, Wheat powdery mildew, Resistance

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