Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (8): 1531-1539.doi: 10.3724/SP.J.1006.2021.04203

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of resistance to leaf scald in newly released sugarcane varieties at seedling stage by artificial inoculation

FU Hua-Ying1(), ZHANG Ting1(), PENG Wen-Jing2, DUAN Yao-Yao1, XU Zhe-Xin1, LIN Yi-Hua1, GAO San-Ji1,*()   

  1. 1National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
    2College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
  • Received:2020-09-04 Accepted:2020-12-01 Online:2021-08-12 Published:2020-12-29
  • Contact: GAO San-Ji E-mail:mddzyfhy@163.com;Zhang_970907@163.com;gaosanji@fafu.edu.cn
  • Supported by:
    Earmarked Fund for the China Agricultural Research System(CARS-170302);Open Project Fund of National Sugarcane Engineering Technology Research Center(2017.3.2)

RichHTML386

14

PDF

715

Abstract:

Sugarcane leaf scald caused by the pathogen of Xanthomonas albilineans is one of important bacterial diseases. The utilization of sugarcane resistant varieties is the most economical and effective measure for control of this disease. In this study, to identify the level of resistance to leaf scald for domestic sugarcane varieties, a total of 49 newly-released sugarcane varieties from different sugarcane breeding institutions in China were identified upon 3-5 fully expanding leaf stage by artificial inoculation with Xa-FJ1 strain. The leaf scald resistant variety LCP 85-384 and the leaf scald susceptible variety ROC20 were used as controls. Our results indicated that the disease indexes of these varieties were continually increased from the 7th day post-inoculation (dpi), while the disease indexes reached a steady plateau at 21-28 dpi. These re-isolated pathogenic bacteria in leaf tissues of the inoculated plants were detected by PCR assay with the XAF1/XAR1 primers specific to X. albilineans. PCR results confirmed that the pathogen Xa-FJ1 was successfully infected with all the tested varieties after inoculation. Based on the disease indexes, 51 sugarcane varieties were divided into four different groups, resistant, medium resistant, susceptible, and high susceptible grades. Of all the tested varieties, 37.3% (19/51) varieties were resistant or medium resistant to leaf scald, among which only three varieties (Yuegan 50, Funong 09-7111, and Zhongzhe 10) reached resistant levels. Meanwhile, 62.7% (32/51) varieties were susceptible or highly susceptible to leaf scald. Our results revealed the lack of high resistance germplasms in the newly released varieties in China, and it is urgent to create more germplasm resources resistant to leaf scald.

Key words: Saccharum spp. hybrid, newly-released varieties, leaf scald, Xanthomonas albilineans, disease resistance identification

Table 1

Disease severity of leaf scald in sugarcane"

分级Grade 症状描述Symptom
0级 Score 0 无症状Asymptomatic
1级 Score 1 1-2条铅笔状白色条纹One or two white pencil lines
2级 Score 2 超过2条铅笔状白色条纹More than two white pencil lines
3级 Score 3 叶片变白或者黄化Chlorotic or yellowing leaf
4级 Score 4 叶片坏死Leaf necrosis
5级 Score 5 植株死亡Plant death

Table 2

Criteria for evaluation of resistance to leaf scald based on disease index in sugarcane"

抗性评价Resistance evaluation 病情指数分级Disease index (%)
高抗High resistant 病情指数 ≤ 5.0 Disease index ≤ 5.0
抗病Resistant 5.0 < 病情指数 ≤ 15.0 5.0 < Disease index ≤ 15.0
中抗Medium resistant 15.0 < 病情指数 ≤ 30.0 15.0 < Disease index ≤ 30.0
感病Susceptible 30.0 < 病情指数 ≤ 50.0 30.0 < Disease index ≤ 50.0
高感High susceptible 病情指数 > 50.0 Disease index > 50.0

Fig. 1

Different disease severity grades of sugarcane leaf scald Score 0: grade 0; Score 1: grade 1; Score 2: grade 2; Score 3: grade 3; Score 4: grade 4; Score 5: grade 5."

Fig. 2

Pathogen isolation and PCR detection of inoculated leaf samples A: colonies isolated from ROC22 with white pencil lines; B: colonies isolated from Mintang 11-610 with white striped leaves; C: colonies isolated from Liucheng 09-15 with chlorotic leaf; D: colonies isolated from Yuegan 49 with yellowing leaf; E: colonies isolated from Funong 11-2907 with yellowing leaf; F: PCR detection for colonies isolated from the above-mentioned samples. 1: D15000+2000 DNA marker; 2: the blank control, sterile H2O; 3: the negative control, healthy leaf sample; 4: the positive control, Xa-FJ1 strain; 5-9: DNA samples from colonies isolated from the above-mentioned samples."

Fig. 3

Disease indexes of 51 sugarcane varieties at different day post-inoculation (dpi)"

Table 3

Grades of resistance to leaf scald by artificial inoculation in sugarcane varieties"

抗病等级
Grade of resistance		 品种(系)名称
Variety (line) name		 品种(系)数量
Number of variety		 病情指数
Disease index (%)		 平均值多重比较
Mean multiple comparison
抗
Resistant		 粤甘50, 福农09-7111, 中蔗10号
Yuegan 50 , Funong 09-7111, Zhongzhe 10		 3 10.6-14.7 12.2 A
中抗
Medium resistant		 中蔗13号, 海蔗28号, 桂糖40号, LCP85-384, 桂糖08-120, 柳城07-150, 云蔗11-3898, 粤甘53, 新台糖22号, 闽糖11-610, 粤甘51, 德蔗12-88, 福农11-2907, 粤甘49, 云蔗09-1601, 粤甘43
Zhongzhe 13, Haizhe 28, Guitang 40, LCP85-384, Guitang 08-120, Liucheng 07-150, Yunzhe 11-3898, Yuegan 53, ROC22, Mintang 11-610, Yuegan 51, Dezhe 12-88, Funong 11-2907, Yuegan 49, Yunzhe 09-1601, Yuegan 43		 16 15.4-28.7 23.9 B
感病
Susceptible		 云蔗11-1204, 云瑞11-450, 粤甘52, 中糖1201, 福农10-0574, 福农38, 中蔗1号, 云蔗11-1074, 福农09-6201, 云蔗08-1609, 福农09-4095, 云瑞12-263, 桂糖46号, 桂糖44号, 粤甘48, 福农07-3206, 福农10-14405, 粤甘46, 福农08-3214, 新台糖20号, 桂糖08-1589, 中蔗6号, 中糖1202, 桂糖11-1076, 桂糖13-386, 柳城09-15, 柳城09-19, 云蔗08-1095
Yunzhe 11-1204, Yunrui 11-450, Yuegan 52, Zhongtang 1201, Funong 10-0574, Funong 38, Zhongzhe 1, Yunzhe 11-1074, Funong 09-6201, Yunzhe 08-1609, Funong 09-4095, Yunrui 12-263, Guitang 46, Guitang 44, Yuegan 48, Funong 07-3206, Funong 10-14405, Yuegan 46, Funong 08-3214, ROC20, Guitang 08-1589, Zhongzhe 6, Zhongtang 1202, Guitang 11-1076, Guitang 13-386, Liucheng 09-15, Liucheng 09-19, Yunzhe 08-1095		 28 30.3-48.4 38.7 C
高感
High susceptible		 云蔗11-3208, 中糖1301, 云瑞10-187, 福农09-12206
Yunzhe 11-3208, Zhongtang 1301, Yunrui 10-187, Funong 09-12206		 4 51.6-58.2 54.6 D

Fig. 4

Cluster analysis of disease resistance to leaf scald in sugarcane cultivars"

[1] Ali A, Khan M, Sharif R, Mujtaba M, Gao S J. Sugarcane omics: an update on the current status of research and crop improvement. Plants, 2019,8:344.
doi: 10.3390/plants8090344
[2] 张跃彬. 我国蔗糖产业安全问题与发展对策. 中国糖料, 2019,41(1):66-68.
Zhang Y B. Safety problems and development countermeasures of cane sugar industry in China. Sugar Crops China, 2019,41(1):66-68 (in Chinese with English abstract).
[3] 刘晓雪, 田冰, 白晨. 我国糖料产业发展特点、问题与趋势. 中国糖料, 2019,41(2):47-51.
Liu X X, Tian B, Bai C. Characteristics, problems and development trends of sugar crop industry in China. Sugar Crops China, 2019,41(2):47-51 (in Chinese with English abstract).
[4] Javed T, Shabbir R, Ali A, Afzal I, Zaheer U, Gao S J. Transcription factors in plant stress responses: challenges and potential for sugarcane improvement. Plants, 2020,9:491.
doi: 10.3390/plants9040491
[5] Barnabas L A, Ramadass R S, Amalraj R S, Palaniyandi M, Rasappa V. Sugarcane proteomics: an update on current status, challenges, and future prospects. Proteomics, 2015,15:1658-1670.
doi: 10.1002/pmic.201400463 pmid: 25641866
[6] Rott P, Davis M J. Leaf scald. In: Rott P, Bailey R A, Comstock J C, Croft B J, Saumtally A S, eds. A Guide to Sugarcane Diseases. Montpellier, France: CIRAD/ISSCT, La Librairie du Cirad, 2000. pp 38-44.
[7] Royer M, Pieretti I, Cociancich S. Recent progress in understanding three major bacterial diseases of sugarcane: Gumming, leaf scald and ratoon stunting. In: Rott P, eds. Achieving Sustainable Cultivation of Sugarcane Volume 2: Breeding, Pests and Diseases. Cambridge, UK: Burleigh Dodds Science Publishing, 2018. pp 311-336.
[8] 孟建玉, 张慧丽, 林岭虹, 黄宏阳, 高三基. 甘蔗白条病及其致病菌Xanthomonas albilineans研究进展. 植物保护学报, 2019,46:257-265.
Meng J Y, Zhang H L, Lin L H, Huang H Y, Gao S J. Research advances in sugarcane leaf scald disease and its causal agent Xanthomonas albilineans. J Plant Prot, 2019,46:257-265 (in Chinese with English abstract).
[9] Lin L H, Ntambo M S, Rott P C, Wang Q N, Lin Y H, Fu H Y, Gao S J. Molecular detection and prevalence of Xanthomonas albilineans, the causal agent of sugarcane leaf scald, in China. Crop Prot, 2018,109:17-23.
doi: 10.1016/j.cropro.2018.02.027
[10] Ntambo M S, Meng J Y, Rott P C, Royer M, Lin L H, Zhang H L, Gao S J. Identification and characterization of Xanthomonas albilineans causing sugarcane leaf scald in China using multilocus sequence analysis. Plant Pathol, 2019,68:269-277.
doi: 10.1111/ppa.12951
[11] Zhang R Y, Wang X Y, Shan H L, Li J, Li W F, Cang X Y, Luo Z M, Yin J, Huang Y K. Identification and phylogenetic analysis of Xanthomonas albilineans (Ashby) Downson based on multiple gene sequences in Yunnan province, China. Sugar Technol, 2019,21:794-801.
doi: 10.1007/s12355-019-00713-0
[12] Klett P, Rott P. Inoculum sources for the spread of leaf scald disease of sugarcane caused by Xanthomonas albilineans in Guadeloupe. J Phytopathol, 1994,142:283-291.
doi: 10.1111/j.1439-0434.1994.tb04541.x
[13] Daugrois J H, Boisne-Noc R, Champoiseau P, Rott P. The revisited infection cycle of Xanthomonas albilineans, the causal agent of leaf scald of sugarcane. Funct Plant Sci Biotechnol, 2012,6:91-97.
[14] Hoy J W, Grisham M P. Sugarcane leaf scald distribution, symptomatology, and effect on yield in Louisiana. Plant Dis, 1994,78:1083.
doi: 10.1094/PD-78-1083
[15] Rott P, Soupa D, Brunet Y, Feldmann P, Letourmy P. Leaf scald (Xanthomonas albilineans) incidence and its effect on yield in seven sugarcane cultivars in Guadeloupe. Plant Pathol, 1995,44:1075-1084.
doi: 10.1111/ppa.1995.44.issue-6
[16] Rott P, Mohamed I S, Klett P, Soupa D, de Saint-Albin A, Feldmann P, Letourmy P. Resistance to leaf scald disease is associated with limited colonization of sugarcane and wild relatives by Xanthomonas albilineans. Phytopathology, 1997,87:1202-1213.
doi: 10.1094/PHYTO.1997.87.12.1202 pmid: 18945019
[17] Lopes S A, Damann K E, Grelen L B. Xanthomonas albilineans diversity and identification based on rep-PCR fingerprints. Current Microbiol, 2001,42:155-159.
doi: 10.1007/s002840010196
[18] Garces F F, Gutierrez A, Hoy J W. Detection and quantification of Xanthomonas albilineans by qPCR and potential characterization of sugarcane resistance to leaf scald. Plant Dis, 2014,98:121-126.
doi: 10.1094/PDIS-04-13-0431-RE pmid: 30708616
[19] Gutierrez A, Garces F F, Hoy J W. Evaluation of resistance to leaf scald by quantitative PCR of Xanthomonas albilineans in sugarcane. Plant Dis, 2016,100:1331-1338.
doi: 10.1094/PDIS-09-15-1111-RE pmid: 30686195
[20] 韦金菊, 魏春燕, 宋修鹏, 覃振强, 谭宏伟, 张荣华, 庞天, 王伦旺, 刘璐, 李杨瑞. 广西北海蔗区甘蔗白条病发生情况调查. 南方农业学报, 2018,49:264-270.
Wei J J, Wei C Y, Song X P, Qin Z Q, Tan H W, Zhang R H, Pang T, Wang L W, Liu L, Li Y R. Sugarcane leaf scald disease in sugarcane planting areas of Beihai, Guangxi. J Southern Agric, 2018,49:264-270 (in Chinese with English abstract).
[21] 李文凤, 单红丽, 张荣跃, 仓晓燕, 王晓燕, 尹炯, 罗志明, 黄应昆. 广西蔗区检测发现检疫性病害甘蔗白条病. 中国农学通报, 2018,34(13):144-149.
Li W F, Shan H L, Zhang R Y, Cang X Y, Wang X Y, Yin J, Luo Z M, Huang Y K. Sugarcane leaf scald disease found in Guangxi sugarcane region. Chin Agric Sci Bull, 2018,34(13):144-149 (in Chinese with English abstract).
[22] Meng J Y, Ntambo M S, Luo L M, Huang M T, Gao S J. Molecular identification of Xanthomonas albilineans infecting elephant grass (Pennisetum purpureum) in China. Crop Prot, 2019,124:104853.
doi: 10.1016/j.cropro.2019.104853
[23] Ntambo M S, Meng J Y, Rott P C, Robert J, Henry, Zhang H L, Gao S J. Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans. Int J Mol Sci, 2019,20:6138.
doi: 10.3390/ijms20246138
[24] Davis M J, Rott P, Baudin P, Dean J L. Evaluation of selective media and immunoassays for detection of Xanthomonas albilineans, the causal agent of sugarcane leaf scald disease. Plant Dis, 1994,78:78-82.
doi: 10.1094/PD-78-0078
[25] Wang Z K, Comstock J C, Hatziloukas E, Schaad N W. Comparison of PCR, BIO-PCR, DIA, ELISA and isolation on semiselective medium for detection of Xanthomonas albilineans, the causal agent of leaf scald of sugarcane. Plant Pathol, 1999,48:245-252.
doi: 10.1046/j.1365-3059.1999.00332.x
[26] Zhang J S, Zhang X T, Tang H B, Zhang Q, Hua X T, Ma X K, Zhu F, Jones T, Zhu X G, Bowers J, Wai C M, Zheng C F, Shil Y, Chen S, Xu X M, Yue J J, Nelsons D R, Huang L X, Li Z, Xu H M, Zhou D, Wang Y J, Hu W C, Lin J S, Deng Y J, Pandey N, Mancini M, Zerpa D, Nguyen J K, Wang L M, Yu L, Xin Y H, Ge L F, Arro J, Han J O, Chakrabarty S, Pushko M, Zhang W P, Ma Y H, Ma P P, Lyu M J, Chen F M, Zheng G Y, Xu J S, Yang Z H, Deng F, Chen X Q, Liao Z Y, Zhang X X, Lin Z C, Lin H, Yan H S, Kuang Z, Zhong W M, Liang P P, Wang G F, Yuan Y, Shi J X, Hou J X, Lin J X, Jin J J, Cao P J, Shen Q C, Jiang Q, Zhou P, Ma Y Y, Zhang X D, Xu R R, Liu J, Zhou Y M, Jia H F, Ma Q, Qi R, Zhang Z L, Fang J P, Fang H K, Song J J, Wang M J, Dong G R, Wang G, Chen Z, Ma T, Liu H, Dhungana S R, Huss S E, Yang X P, Sharma A, Trujillo J H, Hudson M, Riascos J J, Schuler M, Chen L Q, Braun D M, Li L, Yu Q Y, Wang J P, Wang K, Schatz M C, Heckerman D, Van Sluys M A, Souza G M, Moore P H, Sankoff D, VanBuren R, Paterson A H, Nagai C, Ming R. Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L. Nat Genet, 2018,50:1565-1573.
doi: 10.1038/s41588-018-0237-2
[27] Ahmad A, Wang J D, Pan Y B, Sharif R, Gao S J. Development and use of single sequence repeats (SSRs) markers for sugarcane breeding and genetic studies. Agronomy, 2018,8:260.
doi: 10.3390/agronomy8110260
[28] Lakshmanan P, Geijskes R J, Aitken K S, Grof C L P, Bonnett G D, Smith G R. Sugarcane biotechnology: the challenges and opportunities. In Vitro Cell Dev Biol Plant, 2005,41:345-363.
doi: 10.1079/IVP2005643
[29] Patro T S S K, Rao G V N. Reaction of sugarcane clones to leaf scald disease incited by Xanthomonas albilineans. J Mycol Plant Pathol, 2006,36:241-243.
[30] Wang H B, Xiao N Y, Wang Y J, Guo J L, Zhang J S. Establishment of a qualitative PCR assay for the detection of Xanthomonas albilineans (Ashby) Dowson in sugarcane. Crop Prot, 2020,130:105053.
doi: 10.1016/j.cropro.2019.105053
[31] Duarte D V, Fernandez E, Cunha M G, Pieretti I, Hincapie M, Roumagnac P, Comstock J C, Rott P. Comparison of loop-mediated isothermal amplification, polymerase chain reaction, and selective isolation assays for detection of Xanthomonas albilineans from sugarcane. Trop Plant Pathol, 2018,43:351-359.
doi: 10.1007/s40858-018-0216-2
No related articles found!
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd