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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1339-1345.doi: 10.3724/SP.J.1006.2012.01339

• RESEARCH NOTES • Previous Articles    

Inheritance of Resistance to Powdery Mildew in 12 Wheat Cultivars

SONG Feng-Jing1,XIAO Ming-Gang1,HUANG Jiang1,2,3,WANG Xiao-Ming1,ZHU Zhen-Dong1,WU Xiao-Fei1,LI Hong-Jie1,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China; 2 Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China; 3 Graduate School of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-12-30 Revised:2012-04-16 Online:2012-07-12 Published:2012-05-11
  • Contact: 李洪杰, E-mail: hongjie@caas.net.cn

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Abstract: Wheat powdery mildew, caused by the biotrophic parasitic fungus Blumeria graminis (DC.) f. sp. tritici E.O. Speer. is one of the most epidemic diseases in many wheat (Triticum aestivum L.) producing regions in China and other parts of the world. The information on inheritance of resistance to powdery mildew in commercial wheat cultivars is important for controlling the disease and developing new resistant cultivars. In the present study, 17 isolates of B. graminis were used to postulate and analyze powdery mildew resistance genes in 12 wheat cultivars at seedling stage. Molecular detection was performed to disclose the presence of Pm2 and Pm8 for powdery mildew resistance. All of the 12 wheat cultivars were resistant to at least 11 tested isolates. Isolates E09, E20, and Bg2 were used to test F2 populations for analyzing inheritance of powdery mildew resistance in these wheat cultivars. The results demonstrated that single dominant gene(s) was responsible for powdery mildew resistance in these cultivars. The reactions to different isolates of B. graminis and analysis of amplification with Pm2-linked marker Xcfd81showed that Pm2 or its allele was most likely present in Liangxing 66. Using ω-secalin gene- and Glu-B1 gene-specific markers on chromosome arms 1RS and 1BS, respectively, the presence of T1BL·1RS translocation chromosome carrying Pm8 was detected in Shannong 20 and Zhengmai 9962. Since Pm8 was not effective against most isolates tested in this study, other unknown genes for powdery mildew resistance could be present in Shannong 20 and Zhengmai 9962 in addition to Pm8. The reaction patterns of Yanzhan 4110 and Tianmin 668 were identical. The remaining cultivars developed different patterns of reaction to the B. graminis isolates tested.

Key words: Wheat, Powdery mildew, Resistance gene, Inheritance

[1]Zhuang Q-S(庄巧生). Wheat Improvement and Pedigree Analysis in China (中国小麦品种改良及系谱分析). Beijing: China Agriculture Press, 2003 (in Chinese)

[2]Zhan H-X(詹海仙), Chang Z-J(畅志坚), Yang Z-J(杨足君), Zhang H-J(张晖军), Li X(李欣). Sources and evaluation of powdery mildew resistance genes in wheat. Chin Agric Sci Bull (中国农学通报), 2010, 26(10): 42–46 (in Chinese with English abstract)

[3]Wu X-H(吴先华), Luo P-G(罗培高), Yan B-J(晏本菊), Ren Z-L(任正隆). Researches on Powdery mildew resistance genes and its breeding in wheat. Chin Agric Sci Bull (中国农学通报), 2006, 22(5): 346–351 (in Chinese with English abstract)

[4]Zhang H-Q(张海泉). Research advances in molecular breeding of powdery mildew resistance of wheat. Chin J Eco-Agric (中国生态农业学报), 2008, 16(4): 1060–1066 (in Chinese with English abstract)

[5]Li G P, Chen P D, Zhang S Z, Wang X E, He Z H, Zhang Y, Zhao H, Huang H Y, Zhou X C. Effect of the 6VS.6AL translocation on agronomic traits and dough properties of wheat. Euphytica, 2007, 155: 305–313

[6]Wang Z L, Li L H, He Z H, Duan X Y, Zhou Y L, Chen X M, Lillemo M, Singh R P, Wang H., Xia X C. Seedling and adult plant resistance to powdery mildew in Chinese bread wheat cultivars and lines. Plant Dis, 2005, 89: 457–463

[7]Li H-J(李洪杰), Wang X-M(王晓鸣), Song F-J(宋凤景), Wu C-P(伍翠平), Wu X-F(武小菲), Zhang N(张宁), Zhou Y(周阳), Zhang X-Y(张学勇). Response to powdery mildew and detection of resistance genes in wheat cultivars from China. Acta Agron Sin (作物学报), 2011, 37(6): 943–954 (in Chinese with English abstract)

[8]Wu Q-A(吴全安). Methods Used in the Evaluation of Pest Resistant Potentialities in Food Crop Germplasm Resources (粮食作物种质资源抗病虫鉴定方法). Beijing: China Agriculture Press, 1991 (in Chinese)

[9]Qiu Y C, Sun X L, Zhou R H, Kong X Y, Zhang S S, Jia J Z. Identification of microsatellite markers linked to powdery mildew resistance gene Pm2 in wheat. Cereal Res Commun, 2006, 34: 1267–1273

[10]Chai J F, Zhou R H, Jia J Z, Liu X. Development and application of a new codominant PCR marker for detecting 1BL.1RS wheat-rye chromosome translocations. Plant Breed, 2006, 125: 302–304

[11]Cao X-R(曹学仁), Zhou Y-L(周益林), Duan X-Y(段霞瑜), Song Y-L(宋玉立), He W-L(何文兰), Ding K-J(丁克坚), Wang B-T(王保通), Xia X-C(夏先春). Postulation of wheat powdery mildew resistance genes in commercial wheat cultivars and advanced lines from Gansu Province. J Triticeae Crops (麦类作物学报), 2011, 30(5): 948–953 (in Chinese with English abstract)

[12]Li H J, Conner R L, Liu Z Y, Li Y W, Chen Y, Zhou Y L, Duan X Y, Shen T M, Chen Q, Graf R J, Jia X. Characterization of wheat-triticale lines resistant to powdery mildew, stem rust, stripe rust, wheat curl mite, and limitation on spread of WSMV. Plant Dis, 2007, 91: 368–374

[13]Hu T-Z(胡铁柱), Li H-J(李洪杰), Liu Z-J(刘子记), Xie C-J(谢超杰), Zhou Y-L(周益林), Duan X-Y(段霞瑜), Jia X (贾旭), You M-S(尤明山), Yang Z-M(杨作明), Sun Q-X(孙其信), Liu Z-Y(刘志勇). Identification and molecular mapping of the powdery mildew resistance gene in wheat cultivar Yumai 66. Acta Agron Sin (作物学报), 2008, 34(4): 545–550 (in Chinese with English abstract)

[14]Niu J S Wang B Q, Wang Y H, Cao A Z, Qi Z J, Shen T M. Chromosome location and microsatellite markers linked to a powdery mildew resistance gene in wheat line ‘Lankao 90(6)’. Plant Breed, 2008, 127: 346–349
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