Fig. 1

Schematic diagrams of 5' deletion fragments of TaSC promoter with different lengths for constructing GUS reporter vectors"

Fig. 2

Cloning of TaSC promoter A: electrophoretic pattern of genomic DNA of RH8706-49; B: TAIL-PCR amplification of TaSC promoter; C: amplification of TaSC promoter from RH8706-49; M: DL-2000 DNA ladder in 2000, 1000, 750, 500, 200, and 100 bp (from the top to the bottom); 1: profile of the tertiary TAIL-PCR amplification with SP3 and AD4 ( target band shown by the arrow); 2: profile of the secondary TAIL-PCR amplification with SP2 and AD4; 3: the amplification profile with SP3 primer only; 4: Amplification of TaSC promoter ProTaSC (target band shown by the arrow)."

Fig. 3

DNA Sequences of TaSC promoter in salt-tolerant wheat mutant RH8706-49 The amplification fragment of TAIL-PCR is shown by blue italic letters. TATA box and CAAT box are underlined with dotted and solid lines respectively. Shadow shows other functional cis-acting elements whose name remarks under the sequence. Among them, ABRE element (CACGTG) and MBS element (CAACTG) are related to stress response."

Fig. 4

GUS assay results of transgenic Arabidopsis harboring different lengths of ProTaSC promotor fragmentsA: transferred with the 1419 bp promotor; B: transferred with the 1096 bp promotor fragment; C: transferred with the 681 bp promotor fragment; D: transferred with the 343 bp promotor fragment; E: transferred with the 152 bp promotor fragment."

Fig. 5

GUS assay results of different tissues of Arabidopsis harboring full-length promoter ProTaSCA: root; B: stem leaf; C: rosette leaf; D: floral, young pot and stem; E: blooming flower; F: mature pod."

Fig. 6

Quantification of GUS activity in transgenic Arabidopsis under different stresses** and *** indicate significant between the stress treatment and the control (0 h) at the 0.01 and 0.001 probability level, respectively (t-test)."

Supplementary Fig. 6

Alignment of ProTaSC+TaSC sequence from salt-tolerant wheat RH8706-49 and genomic DNA sequence from Chinese spring (Triticum aestivum L.)ProTaSC+TaSC: the cDNA sequence (557 bp) and its promoter sequence (1419 bp) of TaSC gene in RH8706-49. Red bars show the start (ATG) and stop codon (TAA) of TaSC. Traes_5DL_50BA3A: the 4266 bp contiguous genomic DNA sequence of Chinese spring, containing 2290 bp of introns."