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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (04): 614-619.doi: 10.3724/SP.J.1006.2018.00614

• RESEARCH NOTES • Previous Articles     Next Articles

Exploration of Foreign Maize Inbred Lines with Broad Spectrum Resistance to Northern Corn Leaf Blight and Preliminary Identification of Resistance Genes

Ming-Gang XIAO1,2(), Feng-Jing SONG3, Bing SUN2, Xin ZUO4, Guang-Shan ZHAO5, Ai-Hua XIN2, Zhu-Gang LI2,*()   

  1. 1 Postdoctoral Programme, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    2 Heilongjiang Province Cold Region Crop Physiology and Ecology Laboratory / Key Laboratory of Crop Molecular Design and Germplasm Innovation in Heilongjiang Province Tillage and Cultivation Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    3 Qingdao Academy of Agricultural Science, Qingdao 266109, Shandong, China
    4 Ruaral Energy Sources Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    5 Agricultural Technology Extension Station of Jiamusi City, Jiamusi 154002, Heilongjiang, China
  • Received:2017-08-30 Accepted:2018-01-08 Online:2018-01-30 Published:2018-01-30
  • Contact: Zhu-Gang LI E-mail:xiaoyang8076@163.com;lizhugang@163.com
  • Supported by:
    This study was supported by Heilongjiang Postdoctoral Fund (LB-Z14186).


From 2014 to 2016, forty-three maize inbred lines introduced from the United States, France, Russia and Germany, were identified and evaluated for resistance to northern corn leaf blight (NCLB). The results showed that seven, one and six maize inbreds were highly resistant, resistant or moderately resistant to NCLB, accounting for 32.6%. Exserohilum turcicum was used to test the F2 populations for analyzing the inheritance of NCLB resistance in the seven highly resistance maize inbred lines. Genetic analysis suggested that A04, F02, F05, and R01 of these inbred lines probably carried a single dominant gene conferring their resistance to NCLB. The reaction patterns to isolates 0, 1, 2, N, and 123N of E. turcicum showed that new genes resistance to NCLB were most likely present in A04, F02, F05, and R01. The results provided useful information on the introduction of maize germplasms and genetic improvement for resistance to NCLB.

Key words: maize inbred lines, northern corn leaf blight, resistance evaluation, inheritance

Fig. 1

Distribution of northern corn leaf blight resistance score for foreign maize inbred lines in 2014HR: highly resistant; R: resistant; MR: moderately resistant; S: susceptible; HS: highly susceptible."

Fig. 2

Field phenotype of foreign maize inbred lines highly resistant and highly sensitive to northern corn leaf blightA04, G03: inbred lines; HR: highly resistant; HS: highly susceptible."

Table 1

Identification of resistance to northern corn leaf blight of 14 foreign inbred lines across years"

Inbred lines
2014 2015 2016
Lesion area
Lesion area
Lesion area
A02 美国America 15 MR 10 R 17 MR
A04 美国America 1 HR 1 HR 1 HR
A05 美国America 12 MR 12 MR 12 MR
A08 美国America 23 MR 20 MR 25 MR
A11 美国America 5 HR 5 HR 5 HR
A18 美国America 11 MR 11 MR 10 R
A20 美国America 25 MR 25 MR 20 MR
A24 美国America 0 HR 0 HR 0 HR
A28 美国America 13 MR 15 MR 10 R
F02 法国France 1 HR 3 HR 3 HR
F03 法国France 9 R 7 R 9 R
F05 法国France 2 HR 1 HR 1 HR
R01 俄罗斯Russia 4 HR 4 HR 4 HR
G02 德国Germany 0 HR 1 HR 0 HR

Table 2

Reactions of seven F2 populations to Exserohilum turcicum of northern corn leaf blight"

获白/A04 Huobai/A04 448 132 1.5540 0.2125
获白/A11 Huobai/A11 416 168 4.4201 0.0355
获白/A24 Huobai/A24 400 160 3.8095 0.0510
获白/F02 Huobai/F02 438 130 1.3521 0.2449
获白/F05 Huobai/F05 438 142 0.0828 0.7736
获白/R01 Huobai/R01 454 158 0.2179 0.6407
获白/G02 Huobai/G02 432 172 3.8940 0.0485

Table 3

Infection types in the four foreign inbred lines to isolates 0, 1, 2, N, and 123N of northern corn leaf blight"

Inbred line
小种鉴定结果Identification of species
0 1 2 N 123N
A619 S S S S S
A619Ht1 R S R R S
A619Ht2 R R S R S
A619Ht3 R R R R S
A619HtN R R R S S
A04 R R R R R
F02 R R R R R
F05 R R R R R
R01 R R R R R
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