青藏春冬麦区93份小麦地方种质条锈病抗性评价及抗病基因分子鉴定

doi:10.3724/SP.J.1006.2021.01073

Abstract

Abstract:

Wheat stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most serious diseases in wheat. To address the threat of predominant Pst races to wheat production and screen resistance resources to breed new wheat cultivars in China, the resistance of 93 wheat landraces derived from Qinghai-Tibet spring and winter wheat zones to stripe rust were evaluated at seedling stage and adult plant stage, and the Yr genes that they might carry were detected. The resistance of 93 wheat landraces were evaluated at seedling stage in a greenhouse with two Pst races CYR32 and CYR34, and at adult plant stage under four field conditions with the mixture of Pst races (CYR32, CYR33, CYR34, Su11-4, Su11-5, and G22-14) in Chongzhou and Mianyang, Sichuan during 2015-2016, 2017-2018, and 2018-2019 cropping seasons, respectively. The panel of wheat landraces was detected with flanking markers closely linked to stripe rust resistance genes Yr5, Yr10, Yr18, Yr24 (=Yr26), Yr48, Yr65, and Yr67. Resistance evaluation indicated that four landraces (4.30%) were resistant to CYR32, three (3.26%) resistant to CYR34, and one was resistant to both CYR32 and CYR34 at seedling stage. Ten landraces displayed resistance to mixed races at adult plant stage under four field conditions. Molecular detection indicated that 11, 40, and 1 landrace might carry Yr18, Yr48, and Yr65, respectively. Seven landraces may carry both Yr18 and Yr48. In addition, no Yr genes were detected in the three resistant wheat landraces, indicating that these wheat landraces might carry other known or unknown stripe rust resistance genes. The wheat landraces derived from Qinghai-Tibet spring and winter wheat zones showed low levels of resistance to the current predominant Pst races in China, and they carried less resistance genes. These wheat landraces with good resistance and carrying known or unknown resistance genes should be valued to explore novel stripe rust resistance genes and accelerate their utilization in wheat breeding program.

Key words: wheat landraces, stripe rust, resistance evaluation, Yr genes

ZHAO Xu-Yang, YAO Fang-Jie, LONG Li, WANG Yu-Qi, KANG Hou-Yang, JIANG Yun-Feng, LI Wei, DENG Mei, LI Hao, CHEN Guo-Yue. Evaluation of resistance to stripe rust and molecular detection of resistance genes of 93 wheat landraces from the Qinghai-Tibet spring and winter wheat zones[J].Acta Agronomica Sinica, 2021, 47(10): 2053-2063.

Figures/Tables 3

Table 1

Evaluation of stripe rust resistance and molecular detection of Yr genes of 93 wheat landraces from Qinghai-Tibet spring and winter wheat zones"

种质 Landrace	产地 Origin	成株期 Adult plant stage								苗期 Seedling stage			抗性类型 Resistance type				分子标记检测 Presence or absence of Yr gene based on molecular marker detection
		成株期 Adult plant stage							16CZ			16MY			18CZ									19CZ		CYR32	CYR34
		DS(%)	IT	DS(%)	IT	DS(%)	IT	IT		苗期 Seedling stage	IT	IT												Yr18			Yr48		Yr65	Yr67
		长芒白粒麦Changmangbailimai	西藏察雅Chaya, Tibet	40	3	60	3	72	3	3		3			3			S				+			+		—	—
波密杂麦-5 Bomizamai-5	西藏波密Bomi, Tibet	20	2	40	3	100	4	3		3	3			S				+			+		—	—
林芝杂小麦Linzhizaxiaomai	西藏林芝Linzhi, Tibet	80	4	80	3	80	3	3		3	3		S				+			+		—	—
无芒小麦Wumangxiaomai	西藏昂仁Angren, Tibet	*	*	100	4	100	4	4		3	4		S				—			+		+	—
达孜杂小麦-3Dazizaxiaomai-3	西藏达孜Dazi, Tibet	40	3	40	3	60	3	3		4	4		S				—			+		—	—
贡吉卓Gongjizhuo	西藏察隅Chayu, Tibet	100	4	100	4	100	4	4		3	4		S				—			+		—	—
翻身卓Fanshenzhuo	西藏琼结Qiongjie, Tibet	100	4	100	4	84	3	3		3	4		S				—			+		—	—
苏卓卓玛Suzhuozhuoma	西藏八宿Basu, Tibet	20	2	20	3	72	3	1		3	4		S				—			+		—	—
加查扎仁卓玛Jiachazharenzhuoma	西藏加查Jiacha, Tibet	20	2	5	1	8	2	1		2	3		ASR				+			+		—	—
曲下小麦Quxiaxiaomai	西藏定日Dingri, Tibet	80	4	100	3	88	4	3		3	3		S				+			+		—	—
定日长芒Dingrichangmang	西藏定日Dingri, Tibet	80	4	80	4	26	3	3		3	3		S				+			+		—	—
波密短曲Bomiduanqu	西藏波密Bomi, Tibet	60	3	20	3	7	1	3		4	3		S				+			+		—	—
大红麦Dahongmai	青海平安Pingan, Qinghai	5	0;	0	0;	0	0;	1		3	3		APR				+			+		—	—
朗县贡卓Langxiangongzhuo	西藏朗县Langxian, Tibet	5	0;	5	1	10	2	3		3	3		S				+			+		—	—
丕卓Pizhuo	西藏加查Jiacha, Tibet	*	4	60	3	8	1	3		4	3		S				+			+		—	—
广欠无芒Guangqianwumang	西藏林芝Linzhi, Tibet	*	0	0	0	3	1	1		1	3		ASR				+			+		—	—
加查鼠麦Jiachashumai	西藏加查Jiacha, Tibet	*	4	40	3	100	4	3		3	3		S				+			+		—	—
准唐玉卓Huaitangyuzhuo	西藏昂仁Angren, Tibet	100	4	100	4	100	4	4		3	4		S				—			+		—	—
红芒麦子Hongmangmaizi	四川义敦Yidun, Sichuan	20	2	5	1	4	1	1		3	3		APR				+			—		—	—
吉雄扎嘎Jixiongzhaga	西藏贡嘎Gongga, Tibet	5	1	80	4	52	3	3		3	3		S				—			+		—	—
藏麦-169 Zangmai-169	西藏拉萨Lasa, Tibet	100	4	100	4	88	4	4		4	3		S				—			+		—	—
加长麦Jiachangmai	西藏昌都Changdu, Tibet	100	4	100	4	92	4	4		3	3		S				—			+		—	—
踏荣卓Tarongzhuo	西藏尼木Nimu, Tibet	100	4	100	4	15	3	4		3	4		S				+			—		—	—
岗吉基卓Gangjijizhuo	西藏亚东Yadong, Tibet	80	3	60	3	35	3	3		3	3		S				—			—		—	—
小红麦(八宝)Xiaohongmai(Babao)	青海祁连Qilian, Qinghai	60	3	60	3	72	4	1		3	4		S				+			—		—	—
吉林春麦Jilinchunmai	西藏扎囊Zhanang, Tibet	40	2	0	0;	6	1	3		4	4		S				+			—		—	—
下康布Xiakangbu	西藏亚东Yadong, Tibet	20	2	5	1	60	4	2		4	3		S				—			+		—	—
江当秃头Jiangdangtutou	西藏乃东Naidong, Tibet	60	3	40	3	60	4	3		4	4		S				—			+		—	—
长芒毛颖麦Changmangmaoyingmai	西藏察雅Chaya, Tibet	60	3	40	3	80	4	3		2	4		S				—			+		—	—
毛红麦Maohongmai	青海贵德Guide, Qinghai	100	4	100	4	100	4	3		3	3		S				—			—		—	+
尕老汉Galaohan	青海乐都Ledu, Qinghai	5	1	5	1	48	2	1		4	4		APR				—			+		—	—
兰麦Lanmai	青海都兰Dulan, Qinghai	100	4	100	4	100	4	4		4	3		S				—			—		—	+
扎仁玛布Zharenmabu	西藏昌都Changdu, Tibet	80	3	80	3	88	4	4		3	4		S				—			+		—	—
高山早熟小麦Gaoshanzaoshuxiaomai	四川新龙Xinlong, Sichuan	20	2	5	1	13	2	1		3	4		APR				—			+		—	—
小红麦Xiaohongmai	青海都兰Dulan, Qinghai	60	3	10	3	40	3	3		3	4		S				—			+		—	—
大六月黄Daliuyuehuang	青海贵德Guide, Qinghai	80	4	100	4	2	1	1		3	4		S				+			—		—	—
白小麦Baixiaomai	西藏林芝Linzhi, Tibet	80	3	60	3	28	3	3		3	4		S				—			+		—	—
团结基卓Tuanjiejizhuo	西藏察雅Chaya, Tibet	100	4	100	4	72	4	4		4	4		S				—			+		—	—
年扎冬小麦Nianzhadongxiaomai	西藏错那Cuona, Tibet	*	3	20	3	2	2	3		3	3		S				—			—		—	—
吉古丕卓Jigupizhuo	西藏墨竹Mozhu, Tibet	*	3	0	3	*	*	3		3	4		S				—			—		—	—
王卡麦Wangkamai	西藏察雅Chaya, Tibet	*	4	60	3	60	4	3		4	3		S				—			+		—	—
仁布春Renbuchun	西藏仁布Renbu, Tibet	100	4	40	3	28	3	3		3	3		S				—			+		—	—
加查红颖Jiachahongying	西藏加查Jiacha, Tibet	*	4	80	3	76	4	4		3	1		S				—			+		—	—
宗沙麦Zongshamai	西藏察雅Chaya, Tibet	*	4	80	3	88	4	4		4	4		S			—			+		—		—
鲁朗白麦Lulangbaimai	西藏林芝Linzhi, Tibet	40	3	40	3	44	3	3		3	4		S			—			—		+		—
红和尚头Hongheshangtou	西藏乃东Naidong, Tibet	*	4	60	3	800	4	3		3	3		S			—			+		—		—
扎国勾曲麦Zhaguogouqumai	西藏错那Cuona, Tibet	*	4	20	3	60	4	3		3	3		S			—			+		—		—
毛颖麦Maoyingmai	西藏错那Cuona, Tibet	*	4	5	3	30	4	3		3	3		S			—			+		—		—
羊日大穗Yangridasui	西藏拉萨Lasa, Tibet	*	4	0	0;	56	4	3		3	3		S			—			+		—		—
易贡卓Yigongzhuo	西藏波密Bomi, Tibet	*	3	10	3	44	3	3		3	4		S			—			—		+		—
桑久比卓Sangjiubizhuo	西藏察隅Chayu, Tibet	*	3	40	3	60	3	3		4	3		S			—			—		+		—
塔吉卓Tajizhuo	西藏达孜Dazi, Tibet	*	4	100	4	100	4	3		3	4		S			—			+		—		—
曲红麦Quhongmai	西藏拉萨Lasa, Tibet	*	4	40	3	92	4	3		3	4		S			—			+		—		—
错那白麦Cuonabaimai	西藏错那Cuona, Tibet	*	4	10	3	80	4	3		4	4		S			—			—		+		—
白玉小麦Baiyuxiaomai	四川白玉Baiyu, Sichuan	20	2	0	0;	0	0;	2		4	4		APR			—			—		+		—
泽当毛颖Zedangmaoying	西藏乃东Naidong, Tibet	10	2	*	*	37	4	3		3	4		S			—			—		—		—
扎西岗卓Zhaxigangzhuo	西藏定日Dingri, Tibet	100	4	100	4	100	4	4		4	4		S			—			—		—		—
兴荣春麦Xingrongchunmai	西藏隆子Longzi, Tibet	20	3	10	2	10	3	3		3	3		S			—			—		—		—
中芒卓玛Zhongmangzhuoma	西藏芒康Mangkang, Tibet	*	*	60	3	6	2	3		4	4		S			—			—		—		—
然日无芒麦Ranriwumangmai	四川石渠Shiqu, Sichuan	100	4	80	3	100	4	4		3	3		S			—			—		—		—
定结春Dingjiechun	西藏定结Dingjie, Tibet	5	1	5	1	0	0;	1		3	1		ASR			—			—		—		—
德阳杂麦Deyangzamai	西藏米林Milin, Tibet	60	3	60	3	68	4	3		4	4		S			—			—		—		—
白颖无芒小麦Baiyingwumangxiaomai	西藏江孜Jiangzi, Tibet	80	3	80	3	100	4	4		2	0		S			—			—		—		—
生格小麦Shenggexiaomai	西藏昌都Changdu, Tibet	100	4	100	4	*	4	4		4	3		S			—			—		—		—
大白麦Dabaimai	青海化隆Hualong, Qinghai	80	3	60	3	44	3	1		4	4		S			—			—		—		—
红小麦Hongxiaomai	西藏八宿Basu, Tibet	100	4	100	4	88	4	4		4	4		S					—	—		—		—
聂拉木无芒Nielamuwumang	西藏聂拉木Nielamu, Tibet	100	4	100	4	100	4	4		3	4		S					—	—		—		—
沙马比卓Shamabizhuo	西藏察隅Chayu, Tibet	40	3	40	3	48	3	3		3	4		S					—	—		—		—
丁青毛颖麦Dingqingmaoyingmai	西藏昌都Changdu, Tibet	100	4	100	4	76	4	4		3	4		S					—	—		—		—
无芒白麦Wumangbaimai	西藏墨竹Mozhu, Tibet	100	4	100	4	80	4	4		4	3		S					—	—		—		—
泽当杂小麦Zedangzaxiaomai	西藏乃东Naidong, Tibet	*	4	80	4	26	4	3		4	4		S					—	—		—		—
扎仁卓玛Zharenzhuoma	西藏朗县Langxian, Tibet	*	4	80	3	26	3	3		4	4		S					—	—		—		—
东门秃头麦Dongmentutoumai	西藏乃东Naidong, Tibet	*	4	60	3	40	3	3		3	4		S					—	—		—		—
吉丁无芒春麦Jidingwumangchunmai	西藏谢通门Xietongmen, Tibet	*	4	100	4	100	4	4		4	4		S					—	—		—		—
普芒红麦Pumanghongmai	西藏普芒Pumang, Tibet	60	2	5	1	8	1	0;		4	4		APR					—	—		—		—
曲下基卓Quxiajizhuo	西藏拉孜Lazi, Tibet	*	4	80	4	88	4	3		4	4		S					—	—		—		—
沙岗春Shagangchun	西藏康马Kangma, Tibet	100	4	100	4	100	4	3		4	3		S					—	—		—		—
学隆卓Xuelongzhuo	西藏察雅Chaya, Tibet	100	4	5	0	61	3	3		4	3		S					—	—		—		—
白马店卓Baimadianzhuo	西藏林芝Linzhi, Tibet	*	2	5	1	4	1	0;		3	3		APR					—	—		—		—
加查冬春麦Jiachadongchunmai	西藏加查Jiacha, Tibet	100	4	80	3	100	4	3		3	3		S					—	—		—		—
扎娜Zhana	西藏曲松Qusong, Tibet	100	4	20	3	100	4	3		3	3		S					—	—		—		—
杰果扎仁布素Jieguozharenbusu	西藏米林Milin, Tibet	*	3	0	1	17	3	3		3	3		S					—	—		—		—
拉月大穗Layuedasui	西藏林芝Linzhi, Tibet	*	4	40	3	48	3	3		4	3		S					—	—		—		—
长芒培卓Changmangpeizhuo	西藏堆龙Duilong, Tibet	*	4	40	3	18	4	3		3	4		S					—	—		—		—
仁达长光麦Rendachangguangmai	西藏察雅Chaya, Tibet	100	4	100	4	96	4	3		3	3		S					—	—		—		—
东莱长红麦Donglaichanghongmai	西藏加查Jiacha, Tibet	*	4	80	3	96	4	3		3	3		S					—	—		—		—
秃毛麦Tumaomai	西藏波密Bomi, Tibet	*	3	10	3	60	4	3		3	3		S					—	—		—		—
吉日卓Jirizhuo	西藏林芝Linzhi, Tibet	*	3	10	3	25	3	3		3	4		S					—	—		—		—
达当卓Dadangzhuo	西藏察雅Chaya, Tibet	*	4	60	3	88	4	3		3	4		S					—	—		—		—
加查红麦Jiachahongmai	西藏加查Jiacha, Tibet	*	4	100	4	72	4	3		3	4		S					—	—		—		—
无芒毛颖麦Wumangmaoyinmai	西藏加查Jiacha, Tibet	*	4	60	3	76	4	3		4	4		S					—	—		—		—
东门红秃子Dongmenhongtuzi	西藏乃东Naidong, Tibet	*	*	*	*	9	2	3		4	4		S					—	—		—		—
陇南长芒Longnanchangmang	西藏加查Jiacha, Tibet	*	4	100	4	100	4	4		4	4		S					—	—		—		—

Table 1

Table 2

Fig. 1

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Yr 基因 Yr gene	标记类型 Marker type	标记名称 Marker name	引物序列 Primer sequence (5′-3′)	遗传距离 Genetic distance (cM)	参考文献 Reference
Yr5	KASP	Yr5F	GCGCCCCTTTTCGAAAAAATA	—	Marchal et al. ^[29]
		Yr5H	CTAGCATCAAACAAGCTAAATA
		Yr5R	ATGTCGAAATATTGCATAACATGG
Yr10	Indel	Xsdauw79-F	TTGCTCTAAGCTGTGGCCT		Yuan et al. ^[30]
		Xsdauw79-R	GAGTTCAACCCCGAACACT
		Xsdauw79-NF	AGAGCCTAAGCGCCTAAGG
		Xsdauw79-NR	TTAAAATCTCCCAAGTACGCA
Yr18	STS	csLV34	GTTGGTTAAGACTGGTGATGG	0.4	Lagudah et al. ^[31]
	STS	csLV34	TGCTTGCTATTGCTGAATAGT
	Gene Marker	Cssfr5	TTGATGAAACCAGTTTTTTTTCTA	—	Lagudah et al. ^[31]
			GGGAGCATTATTTTTTTCCATCATG
			ACTTTCCTGAAAATAATACAAGCA
			TATGCCATTTAACATAATCATGAA
Yr24	STS	Xwe173	GGGACAAGGGGAGTTGAAGC	1.4	Wang et al. ^[32]
	STS	Xwe173	GAGAGTTCCAAGCAGAACAC
	STS	Xwe33	TAAACCAAGTCCCCCAAA	—	Wang et al. ^[32]
	STS	Xwe33	GGAGTCCATCTTCACCGA
Yr48	SSR	Xwmc727	CATAATCAGGACAGCCGCAC	4.4	Lowe et al. ^[33]
Yr48	SSR	Xwmc727	TAGTGGCCTGATGTATCTAGTTGG
Yr65	SSR	Xgwm18	GGTTGCTGAAGAACCTTATTTAGG	1.2	Cheng et al. ^[34]
Yr65	SSR	Xgwm18	TGGCGCCATGATTGCATTATCTTC
Yr67	SSR	Xbarc182	CCATGGCCAACAGCTCAAGGTCTC	0.4	Xu et al. ^[35]
	SSR	Xbarc182	CGCAAAACCGCATCAGGGAAGCACCAAT
	SSR	Xcfa2040	TCAAATGATTTCAGGTAACCACTA	2.8	Xu et al. ^[35]
	SSR	Xcfa2040	TTCCTGATCCCACCAAACAT

Evaluation of resistance to stripe rust and molecular detection of resistance genes of 93 wheat landraces from the Qinghai-Tibet spring and winter wheat zones

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