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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (11): 1643-1649.doi: 10.3724/SP.J.1006.2017.01643


Mapping Stripe Rust Resistance Gene YrZM103 in Wheat Cultivar Zhengmai 103 by BSR-Seq

ZHANG Huai-Zhi1,XIE Jing-Zhong2,CHEN Yong-Xing2,LIU Xu3,WANG Yong1,WU Qiu-Hong2,Lu Ping2,ZHANG De-Yun1,LI Miao-Miao1,GUO Guang-Hao1,YAN Su-Hong3,YANG Zhao-Sheng3,ZHAO Hong4,WANG Xi-Cheng4,JIA Lianhe5,CAO Ting-Jie4,*,LIU Zhi-Yong2,*   

  1. 1 College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China; 2 State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3 Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; 4Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 5Tangxian Vocational and Technical Educational Center, Tangxian, Hebei 072350, China
  • Received:2017-01-13 Revised:2017-07-23 Online:2017-11-12 Published:2017-08-10
  • Contact: 刘志勇, E-mail: zyliu@genetics.ac.cn; 曹廷杰, E-mail: caotingjie893@163.com E-mail:huaizhizhang0066@163.com
  • Supported by:

    This study was supported by the National Key Research and Development Program of China (2017YFD0101802).


Zhengmai 103 is a new wheat cultivar highly resistant to stripe rust. In order to detect the stripe rust resistance gene in Zhengmai 103, a segregating population was developed by making cross between Zhengmai 103 and a highly stripe rust susceptible wheat cultivar Nongda 399. The 214 F2 derived F2:3 progenies were inoculated with mixed prevailing Pst races CYR32, CYR33 and CYR34 (V26) in field condition for disease resistance evaluation at adult plant stage. Genetic analysis revealed that the stripe rust resistance of Zhengmai 103 is controlled by a single gene, temporarily designated YrZM103. By applying RNA-seq with bulked segregant analysis (BSA), six polymorphic markers were developed to map YrZM103 on chromosome 7BL flanked by markers ZM215 and ZM221 with genetic distances of 6.9 and 11.8 cM, respectively. Comparative genetic mapping indicated YrZM103 was located on different genetic interval from that of known stripe rust resistance genes on 7BL.

Key words: Zhengmai 103, Stripe rust, Molecular marker, BSR-Seq, Wheat

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